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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 57(9)

Confirmation of QTL mapping and marker validation for partial seedling resistance to crown rot in wheat line ‘2-49’

B. C. Y. Collard A C, R. Jolley A, W. D. Bovill A, R. A. Grams A, G. B. Wildermuth B, M. W. Sutherland A D

A Centre for Systems Biology (formerly, Centre for Rural and Environmental Biotechnology), Faculty of Sciences, University of Southern Queensland, Toowoomba, Qld 4350, Australia.
B Leslie Research Centre, Queensland Department of Primary Industries and Fisheries, Toowoomba, Qld 4350, Australia.
C Present address: Hermitage Research Station, Queensland Department of Primary Industries and Fisheries, Warwick, Qld 4370, Australia.
D Corresponding author. Email: marksuth@usq.edu.au
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We have tested the efficacy of putative microsatellite single sequence repeat (SSR) markers, previously identified in a 2-49 (Gluyas Early/Gala) × Janz doubled haploid wheat (Triticum aestivum) population, as being linked to partial seedling resistance to crown rot disease caused by Fusarium pseudograminearum. The quantitative trait loci (QTLs) delineated by these markers have been tested for linkage to resistance in an independent Gluyas Early × Janz doubled haploid population. The presence of a major QTL on chromosome 1DL (QCr.usq-1D1) and a minor QTL on chromosome 2BS (QCr.usq-2B1) was confirmed. However, a putative minor QTL on chromosome 2A was not confirmed. The QTL on 1D was inherited from Gluyas Early, a direct parent of 2-49, whereas the 2B QTL was inherited from Janz. Three other putative QTLs identified in 2-49 × Janz (on 1AL, 4BL, and 7BS) were inherited by 2-49 from Gala and were not able to be confirmed in this study. The screening of SSR markers on a small sample of elite wheat genotypes indicated that not all of the most tightly linked SSR markers flanking the major QTLs on 1D and 1A were polymorphic in all backgrounds, indicating the need for additional flanking markers when backcrossing into some elite pedigrees. Comparison of SSR haplotypes with those of other genotypes exhibiting partial crown rot resistance suggests that additional, novel sources of crown rot resistance are available.

Keywords: targetted mapping, QTL resolution, 1-LOD support interval, allelic homoplasy.

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