Expression analysis of Pisum sativum putative defence genes during Orobanche crenata infection
José Vicente Die A C , Belén Román A , Salvador Nadal A , Miguel Á. Dita B and Clara I. González-Verdejo AA IFAPA ‘Alameda del Obispo’, Mejora y Biotecnología. Apdo. 4084, 14080 Córdoba, Spain.
B Embrapa Mandioca Tropical, Rua Embrapa s/n, 44380-000 Cruz das Almas, Brazil.
C Corresponding author. Email: josev.die@juntadeandalucia.es
Crop and Pasture Science 60(5) 490-498 https://doi.org/10.1071/CP08274
Submitted: 18 August 2008 Accepted: 23 February 2009 Published: 14 May 2009
Abstract
The root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental issue for the genetic improvement of legumes. In this work, the temporal expression patterns of 8 defence-genes known to be involved in different metabolic pathways activated during several plant–pathogen interactions were investigated in Pisum sativum. Molecular analyses were carried out using quantitative real-time polymerase chain reaction during the initial stages of the parasitisation process in susceptible (Messire) and incompletely resistant (Ps624) pea genotypes. Transcriptional changes in response to O. crenata revealed induction of genes putatively encoding pathogenesis-related proteins, peroxidase activity, and dehydration stress-responsive signalling. This, combined with high constitutive gene expression mediating the phenylpropanoid pathway were observed as part of the defence mechanisms triggered in Ps624 to restrict the growth of the parasite.
Additional keywords: parasitic plants, plant defence, real-time PCR.
Acknowledgments
The authors acknowledge the financial support provided by the Spanish project INIA2007-0009.
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