Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Proteomic prospects for tolerance of sunflower (Helianthus annuus) to drought stress during the flowering stage

Mehdi Ghaffari A C , Mahmoud Toorchi B , Mostafa Valizadeh B and Mohammadreza Shakiba B
+ Author Affiliations
- Author Affiliations

A Seed and Plant Improvement Institute, Agricultural Research Education and Extension Organisation (AREEO), Karaj, Iran.

B Department of Crop Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666-16471, Iran.

C Corresponding author. Email: ghaffari@areeo.ac.ir

Crop and Pasture Science 68(5) 457-465 https://doi.org/10.1071/CP17105
Submitted: 28 February 2017  Accepted: 15 May 2017   Published: 19 June 2017

Abstract

In order to understanding proteomic basis of drought tolerance in sunflower (Helianthus annuus L.), two contrasting inbred lines were subjected to drought stress during the flowering stage for two years. Proteins were extracted from leaves of well-watered and drought-treated plants by using the TCA–acetone precipitation method and analysed by two-dimensional polyacrylamide gel electrophoresis followed by nanoscale liquid chromatography coupled to tandem mass spectrometry for identification of affected proteins. When comparing proteomic patterns, 18 proteins were changed by drought stress in sensitive lines and 24 proteins in tolerant lines. Concurrent down-expressions of oxygen-evolving enhancer and ferredoxin-NADP reductase were considered as primary drought sensors that mediate downstream pathways to cope with drought conditions. Differential and line-specific proteomic changes were attributed as the source for contrasting response to drought stress. According to the results, scavenging of reactive oxygen species, conservation of energy and water, and cell-structure integrity constituted the major aspects of drought tolerance in sunflower.

Additional keywords: drought resistance, nano-LC–MS/MS, photosynthesis, proteomics, ROS, 2-DE.


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