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Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Differential nitrogen supply causes large variability in photosynthetic traits in wheat germplasm

Szilvia Veres A B C , Al Imran Malik B and Zed Rengel B
+ Author Affiliations
- Author Affiliations

A Department of Agricultural Botany, Crop Physiology and Biotechnology, Institute of Crop Sciences, University of Debrecen, Böszörményi 138, Debrecen, Hungary.

B School of Agriculture and Environment, University of Western Australia, Stirling Highway, Crawley, WA 6009, Australia.

C Corresponding author. Email: szveres@agr.unideb.hu

Crop and Pasture Science 68(8) 703-712 https://doi.org/10.1071/CP17126
Submitted: 24 March 2017  Accepted: 12 August 2017   Published: 21 September 2017

Abstract

Increased food production and enhanced sustainability depend on improving nitrogen-use efficiency (NUE) of crops. Breeding for enhanced NUE can take advantage of doubled-haploid populations derived from parents differing in the trait. This study evaluated variation in photosynthetic parameters at various growth stages in 43 wheat genotypes (parents of the existing doubled-haploid mapping populations) under optimal and low (one-quarter of the optimal) N supply. For relative chlorophyll content, the genotype × N treatment interaction was significant at tillering, booting, pre-anthesis and anthesis. Genotypes with small differences in relative chlorophyll content between the two N supplies were CD87 at tillering and pre-anthesis, and Batavia at anthesis. Potential photochemical activity (Fv/Fm) was measured at tillering and anthesis. The genotype × N treatment interaction was significant in both growth stages. Based on net photosynthesis, stomatal conductance and intrinsic water usez efficiency, there was variable potential of the genotypes to cope with low N supply; significant differences were found among genotypes at ambient CO2 and between N treatments at elevated CO2 concentration (2000 µmol mol–1) for all three parameters. Based on all studied parameters, a dissimilarity matrix was constructed, separating the 43 genotypes into four groups. Group 2 comprised 15 of the genotypes (Batavia, Beaver, Calingiri, CD87, Frame, Krichauff, Neepawa, Soissons, Spear, Stiletto, WAWHT2036, WAWHT2074, Westonia, Wilgoyne, Yitpi), characterised by small differences in relative chlorophyll content and Fv/Fm caused by different N supply at tillering and anthesis. These genotypes therefore appear to have relative tolerance to low N supply and a potential to be used in discerning the molecular basis of tolerance to low N supply.

Additional keywords: chlorophyll fluorescence, intrinsic water-use efficiency, relative chlorophyll content, stomatal conductance.


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