Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Zinc and iron application in conjunction with nitrogen for agronomic biofortification of field crops – a review

Amandeep Kaur A and Guriqbal Singh https://orcid.org/0000-0002-2274-2342 B *
+ Author Affiliations
- Author Affiliations

A Department of Agronomy, Punjab Agricultural University, Ludhiana 141004, India.

B Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana 141004, India.

* Correspondence to: singhguriqbal@pau.edu

Handling Editor: Shahid Hussain

Crop & Pasture Science - https://doi.org/10.1071/CP21487
Submitted: 30 June 2021  Accepted: 17 May 2022   Published online: 24 June 2022

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context: Maintaining food and nutritional security for the ever increasing population of the world is a great chllenge. Zinc and iron are important micronutrients for both human health and plant growth. Insufficient intake of these micronutrients leads to their deficiency in human body which causes morbidity and mortality in different age groups of poor populations in developing countries.

Aims: Therefore, agronomic biofortification is considered the most promising approach to alleviate zinc and iron malnutrition in developing countries.

Methods: The studies reviewed in this article clearly show that the combined application of zinc and nitrogen, iron and nitrogen, and zinc, iron and nitrogen to the soil or to the foliage during the reproductive phase leads to enhanced nutrient (zinc and iron) content in edible parts of crop plants. This happens as the remobilisation and translocation of zinc, iron and urea are governed by the same genetic pathways inside the plant.

Key results: The soil/foliar application of micronutrients (zinc and iron) along with nitrogen (mainly through urea) improves not only the micronutrient (zinc and iron) content in edible parts of the crop plants but also the crop productivity, and thus, is a win–win situation for growers as well as consumers.

Conclusions: Foliar application of urea at 1–2% along with zinc or iron or both during the reproductive phase has been found more effective for biofortification point of view.

Implications: This article reviews the effects of zinc and iron application in combination with nitrogen on agronomic biofortification in important field crops.

Keywords: cereals, foliar application, grain biofortification, grain yield, iron, micronutrients, pulses, zinc.


References

Abdelgader EO (2007) Effect of different nitrogen sources on growth and yield of maize (Zea mays L.). Unpublished MSc Thesis, Omdurman Islamic University, Faculty of Agriculture, Sudan.

Aciksoz SB, Yazici A, Ozturk L, Cakmak I (2011) Biofortification of wheat with iron through soil and foliar application of nitrogen and iron fertilizers. Plant and Soil 349, 215–225.
Biofortification of wheat with iron through soil and foliar application of nitrogen and iron fertilizers.Crossref | GoogleScholarGoogle Scholar |

Aggarwal N, Upadhyay P, Tigadi SB (2020) Biofortification to improve nutrition: a review. International Journal of Current Microbiology and Applied Sciences 9, 763–779.
Biofortification to improve nutrition: a review.Crossref | GoogleScholarGoogle Scholar |

Akhter S, Rasool R, Nabi A, Yousuf V, Dar NA, Sofi KA, Munshi R, Nadeem M (2020) Agronomic biofortification in rice with Zn. International Journal of Current Microbiology and Applied Sciences 9, 2995–3008.
Agronomic biofortification in rice with Zn.Crossref | GoogleScholarGoogle Scholar |

Akram MA, Depar N, Memon MY (2017) Synergistic use of nitrogen and zinc to bio-fortify zinc in wheat grains. Eurasian Journal of Soil Science 6, 319–326.
Synergistic use of nitrogen and zinc to bio-fortify zinc in wheat grains.Crossref | GoogleScholarGoogle Scholar |

Alloway BJ (2008) ‘Zn in soils and crop nutrition.’ 2nd edn. (International Zinc Association (IZA) and International Fertilizer Association (IFA): Brussels, Belgium and Paris, France)

Arabhanvi F, Hulihalli UK (2018) Agronomic fortification with zinc and iron to enhancing micronutrient concentration in sweet corn grain to ameliorate the deficiency symptoms in human beings. International Journal of Current Microbiology and Applied Sciences 7, 333–340.
Agronomic fortification with zinc and iron to enhancing micronutrient concentration in sweet corn grain to ameliorate the deficiency symptoms in human beings.Crossref | GoogleScholarGoogle Scholar |

Arif M, Dashora LN, Choudhary J, Kadam SS, Mohsin M (2019) Effect of varieties and nutrient management on quality and zinc biofortification of wheat (Triticum aestivum). Indian Journal of Agricultural Sciences 89, 1472–1476.

Augustine R, Kalyanasundaram D (2021) Effect of agronomic biofortification on growth, yield, uptake and quality characters of maize (Zea mays L.) through integrated management practices under North-eastern region of Tamil Nadu, India. Journal of Applied and Natural Science 13, 278–286.
Effect of agronomic biofortification on growth, yield, uptake and quality characters of maize (Zea mays L.) through integrated management practices under North-eastern region of Tamil Nadu, India.Crossref | GoogleScholarGoogle Scholar |

Bajiya R, Yadav RK (2017) Bio-fortification for enhancing nutritional quality of pulses under climate change. Annals of Agricultural and Biological Research 22, 174–176.

Barut H (2019) Effects of foliar urea, potassium and zinc sulphate treatments before and after flowering on grain yield, technological quality and nutrient concentrations of wheat. Applied Ecology and Environmental Research 17, 4325–4342.
Effects of foliar urea, potassium and zinc sulphate treatments before and after flowering on grain yield, technological quality and nutrient concentrations of wheat.Crossref | GoogleScholarGoogle Scholar |

Botoman L, Nalivata PC, Chimungu JG, Munthali MW, Bailey EH, Ander EL, Lark RM, Mossa A-W, Young SD, Broadley MR (2020) Increasing zinc concentration in maize grown under contrasting soil types in Malawi through agronomic biofortification: trial protocol for a field experiment to detect small effect sizes. Plant Direct 4, e00277
Increasing zinc concentration in maize grown under contrasting soil types in Malawi through agronomic biofortification: trial protocol for a field experiment to detect small effect sizes.Crossref | GoogleScholarGoogle Scholar | 33103047PubMed |

Bouis HE (2002) Plant breeding: a new tool for fighting micronutrient malnutrition. Journal of Nutrition 132, 491S–494S.
Plant breeding: a new tool for fighting micronutrient malnutrition.Crossref | GoogleScholarGoogle Scholar | 11880577PubMed |

Cakmak I (2010) Biofortification of cereals with zinc and iron through fertilization strategy. In ‘19th world congress of soil science, soil solutions for a changing world’. 1–6 August 2010, Brisbane, Qld, pp. 4–6. (CD-ROM)

Cakmak I, Kutman UB (2018) Agronomic biofortification of cereals with zinc: a review. European Journal of Soil Science 69, 172–180.
Agronomic biofortification of cereals with zinc: a review.Crossref | GoogleScholarGoogle Scholar |

Cakmak I, Torun A, Millet E, Feldman M, Fahima T, Korol A, Nevo E, Barun HJ, Özkan H (2004) Triticum dicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat. Soil Science and Plant Nutrition 50, 1047–1054.
Triticum dicoccoides: an important genetic resource for increasing zinc and iron concentration in modern cultivated wheat.Crossref | GoogleScholarGoogle Scholar |

Cakmak I, Pfeiffer WH, McClafferty B (2010a) REVIEW: Biofortification of durum wheat with zinc and iron. Cereal Chemistry 87, 10–20.
REVIEW: Biofortification of durum wheat with zinc and iron.Crossref | GoogleScholarGoogle Scholar |

Cakmak I, Kalayci M, Kaya Y, Torun AA, Aydin N, Wang Y, Arisoy Z, Erdem H, Yazici A, Gokmen O, Ozturk L, Horst WJ (2010b) Biofortification and localization of zinc in wheat grain. Journal of Agricultural and Food Chemistry 58, 9092–9102.
Biofortification and localization of zinc in wheat grain.Crossref | GoogleScholarGoogle Scholar | 23654236PubMed |

Cambraia TLL, Fontes RLF, Vergütz L, Vieira RF, Neves JCL, Corrêa Netto PS, Dias RFN (2019) Agronomic biofortification of common bean grain with zinc. Pesquisa Agropecuária Brasileira 54, e01003
Agronomic biofortification of common bean grain with zinc.Crossref | GoogleScholarGoogle Scholar |

Chowdhury AP, Biswas A, Mandal P (2018) Response of nitrogen and zinc fertilization on nutrient uptake and seed quality of late sown wheat. Journal of Sylhet University of Agriculture 5, 181–188.

Das S, Chaki AK, Hossain A (2019) Breeding and agronomic approaches for the biofortification of zinc in wheat (Triticum aestivum L.) to combat zinc deficiency in millions of a population: a Bangladesh perspective. Acta Agrobotanica 72, 1770
Breeding and agronomic approaches for the biofortification of zinc in wheat (Triticum aestivum L.) to combat zinc deficiency in millions of a population: a Bangladesh perspective.Crossref | GoogleScholarGoogle Scholar |

Das S, Jahiruddin M, Islam MR, Mahmud AA, Hossain A, Laing AM (2020) Zinc biofortification in the grains of two wheat (Triticum aestivum L.) varieties through fertilization. Acta Agrobotanica 73, 7312
Zinc biofortification in the grains of two wheat (Triticum aestivum L.) varieties through fertilization.Crossref | GoogleScholarGoogle Scholar |

Dash D, Patro H, Tiwari RC, Shahid M (2010) Effect of organic and inorganic sources of nitrogen on Fe, Mn, Cu and Zn uptake and content of rice grain at harvest and straw at different stages of rice (Oryza sativa) crop growth. Advances in Applied Science Research 1, 36–49.

de Valença AW, Bake A, Brouwer ID, Giller KE (2017) Agronomic biofortification of crops to fight hidden hunger in sub-Saharan Africa. Global Food Security 12, 8–14.
Agronomic biofortification of crops to fight hidden hunger in sub-Saharan Africa.Crossref | GoogleScholarGoogle Scholar |

Deshlahare H, Banjara GP (2019) Impact of biofortification of zinc and iron on growth parameters and yields of chickpea (Cicer arietinum L.) through agronomic intervention in Chhattisgarh plains. Journal of Pharmacognosy and Photochemistry 8, 383–386.

Dey S, Prasad S, Chichaghare AR (2020) Effect of placement and foliar application of urea, KCl & Zn (supplementary nourishment) on growth and yield of cowpea (Vigna unguiculata L.) under guava (Psidium guajava L.) based agri-horti system. Current Journal of Applied Science and Technology 39, 61–67.
Effect of placement and foliar application of urea, KCl & Zn (supplementary nourishment) on growth and yield of cowpea (Vigna unguiculata L.) under guava (Psidium guajava L.) based agri-horti system.Crossref | GoogleScholarGoogle Scholar |

Dhaliwal SS, Sharma V, Shukla AK, Verma V, Kaur M, Shivay YS, Nisar S, Gaber A, Brestic M, Barek V, Skalicky M, Ondrisik P, Hossain A (2022) Biofortification—A frontier novel approach to enrich micronutrients in field crops to encounter the nutritional security. Molecules 27, 1340
Biofortification—A frontier novel approach to enrich micronutrients in field crops to encounter the nutritional security.Crossref | GoogleScholarGoogle Scholar | 35209127PubMed |

Distelfeld A, Cakmak I, Peleg Z, Ozturk L, Yazici AM, Budak H, Saranga Y, Fahima T (2007) Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations. Physiologia Plantarum 129, 635–643.
Multiple QTL-effects of wheat Gpc-B1 locus on grain protein and micronutrient concentrations.Crossref | GoogleScholarGoogle Scholar |

El Habbasha SF, Mohamed MH, Abd El-Lateef EM, Mekki BB, Ibrahim ME (2013) Effect of combined zinc and nitrogen on yield, chemical constituents and nitrogen use efficiency of some chickpea cultivars under sandy conditions. World Journal of Agricultural Sciences 9, 354–360.
Effect of combined zinc and nitrogen on yield, chemical constituents and nitrogen use efficiency of some chickpea cultivars under sandy conditions.Crossref | GoogleScholarGoogle Scholar |

Erenoglu EB, Kutman UB, Ceylan Y, Yildiz B, Cakmak I (2011) Improved nitrogen nutrition enhances root uptake, root-to-shoot translocation and remobilization of zinc (65Zn) in wheat. New Phytologist 189, 438–448.
Improved nitrogen nutrition enhances root uptake, root-to-shoot translocation and remobilization of zinc (65Zn) in wheat.Crossref | GoogleScholarGoogle Scholar | 21029104PubMed |

Esmaeili M, Heidarzade A, Gholipour M (2016) Response of maize to foliar application of zinc and Azotobacter inoculation under different levels of urea fertilizer. Journal of Agricultural Sciences 61, 151–162.

Fan M-S, Zhao F-J, Fairweather-Tait SJ, Poulton PR, Dunham SJ, McGrath SP (2008) Evidence of decreasing mineral density in wheat grain over the last 160 years. Journal of Trace Elements in Medicine and Biology 22, 315–324.
Evidence of decreasing mineral density in wheat grain over the last 160 years.Crossref | GoogleScholarGoogle Scholar | 19013359PubMed |

García-Bañuelos ML, Sida-Arreola JP, Sánchez E (2014) Biofortification: promising approach to increasing the content of Fe and Zn in staple food crops. Journal of Elementary Science Education 19, 865–888.
Biofortification: promising approach to increasing the content of Fe and Zn in staple food crops.Crossref | GoogleScholarGoogle Scholar |

Garg M, Sharma N, Sharma S, Kapoor P, Kumar A, Chunduri V, Arora P (2018) Biofortified crops generated by breeding, agronomy, and transgenic approaches are improving lives of millions of people around the world. Frontiers in Nutrition 5, 1–12.
Biofortified crops generated by breeding, agronomy, and transgenic approaches are improving lives of millions of people around the world.Crossref | GoogleScholarGoogle Scholar |

Garvin DF, Welch RM, Finley JW (2006) Historical shifts in the seed mineral micronutrient concentration of US hard red winter wheat germplasm. Journal of the Science of Food and Agriculture 86, 2213–2220.
Historical shifts in the seed mineral micronutrient concentration of US hard red winter wheat germplasm.Crossref | GoogleScholarGoogle Scholar |

Gonzalez D, Almendros P, Obrador A, Alvarez JM (2019) Zinc application in conjunction with urea as a fertilization strategy for improving both nitrogen use efficiency and the zinc biofortification of barley. Journal of the Science of Food and Agriculture 99, 4445–4451.
Zinc application in conjunction with urea as a fertilization strategy for improving both nitrogen use efficiency and the zinc biofortification of barley.Crossref | GoogleScholarGoogle Scholar | 30866046PubMed |

Goudia BD, Hash CT (2015) Breeding for high grain iron and zinc levels in cereals. International Journal of Innovation and Applied Studies 12, 342–354.

Grujcic D, Yazici AM, Tutus Y, Cakmak I, Singh BR (2021) Biofortification of silage maize with zinc, iron and selenium as affected by nitrogen fertilization. Plants 10, 391
Biofortification of silage maize with zinc, iron and selenium as affected by nitrogen fertilization.Crossref | GoogleScholarGoogle Scholar | 33670608PubMed |

Gulave CM, Kshirsagar AV (2020) Biofortified varieties: way to build nutritional immunity to fight against Covid-19 pandemic – a review. Journal of Pharmacognosy and Photochemistry 9, 219–221.
Biofortified varieties: way to build nutritional immunity to fight against Covid-19 pandemic – a review.Crossref | GoogleScholarGoogle Scholar |

Haider MU, Farooq M, Nawaz A, Hussain M (2018) Foliage applied zinc ensures better growth, yield and grain biofortification of mungbean. International Journal of Agriculture and Biology 20, 2817–2822.

Hanjagi PS, Singh B (2018) Nitrogen nutrition of crops: a critical determinant of plant iron and zinc uptake and their biofortification in grains of wheat. Journal of Pharmacognosy and Phytochemistry 7, 94–104.

Hanumanthappa D Hanumanthappa D (2018) Enrichment of iron and zinc content in pigeonpea genotypes through agronomic biofortification to mitigate malnutrition. International Journal of Current Microbiology and Applied Sciences 7, 4334–4342.

Hao H-L, Wei Y-Z, Yang X-e, Feng Y, Wu C-Y (2007) Effects of different nitrogen fertilizer levels on Fe, Mn, Cu and Zn concentrations in shoot and grain quality in rice (Oryza sativa). Rice Science 14, 289–294.
Effects of different nitrogen fertilizer levels on Fe, Mn, Cu and Zn concentrations in shoot and grain quality in rice (Oryza sativa).Crossref | GoogleScholarGoogle Scholar |

Hossain A, Mottaleb KA, Farhad M, Barma NCD (2019) Mitigating the twin problems of malnutrition and wheat blast by one wheat variety, ‘BARI Gom 33’, in Bangladesh. Acta Agrobotanica 72, 1775
Mitigating the twin problems of malnutrition and wheat blast by one wheat variety, ‘BARI Gom 33’, in Bangladesh.Crossref | GoogleScholarGoogle Scholar |

Hussain S, Sahar K, Naeem A, Zafar-ul-Hye M, Aon M (2018) Combined zinc and nitrogen applications at panicle initiation for zinc biofortification in rice. Periodicum Biologorum 120, 105–110.
Combined zinc and nitrogen applications at panicle initiation for zinc biofortification in rice.Crossref | GoogleScholarGoogle Scholar |

Hussain M, Shahid MZ, Mehboob N, Minhas WA, Akram M (2021) Zinc application improves growth, yield and grain zinc concentration of mung bean (Vigna radiata L.). Semina: Ciências Agrárias 42, 487–500.

Iqbal J, Khan R, Wahid A, Sardar K, Khan N, Ali M, Hussain M, Ali W, Ali M, Ahmad R (2016) Effect of nitrogen and zinc on maize (Zea mays L.) yield components and plant concentration. Advances in Environmental Biology 10, 203–208.

Jalal A, Shah S, Teixeira Filho MCM, Khan A, Shah T, Hussain Z, Younis M, Ilyas M (2020a) Yield and phenological indices of wheat as affected by exogenous fertilization of zinc and iron. Revista Brasileira de Ciências Agrárias 15, e7730
Yield and phenological indices of wheat as affected by exogenous fertilization of zinc and iron.Crossref | GoogleScholarGoogle Scholar |

Jalal A, Shah S, Teixeira Filho MCM, Khan A, Shah T, Ilyas M, Rosa PAL (2020b) Agro-biofortification of zinc and iron in wheat grains. Gesunde Pflanzen 72, 227–236.
Agro-biofortification of zinc and iron in wheat grains.Crossref | GoogleScholarGoogle Scholar |

Jalal A, Galindo FS, Boleta EHM, Oliveira CEdS, Reis ARd, Nogueira TAR, Moretti Neto MJ, Mortinho ES, Fernandes GC, Teixeira Filho MCM (2021) Common bean yield and zinc use efficiency in association with diazotrophic bacteria co-inoculations. Agronomy 11, 959
Common bean yield and zinc use efficiency in association with diazotrophic bacteria co-inoculations.Crossref | GoogleScholarGoogle Scholar |

Jha AB, Warkentin TD (2020) Biofortification of pulse crops: status and future perspectives. Plants 9, 73
Biofortification of pulse crops: status and future perspectives.Crossref | GoogleScholarGoogle Scholar |

Jose A, Jacob D, Bindhu JS, Meera AV (2021) Biofortification of rice grain with zinc through inorganic fertilization. Journal of Tropical Agriculture 59, 286–291.

Kanwal A, Khan MB, Hussain M, Naeem M, Rizwan MS, Zafar-ul-Hye M (2020) Basal application of zinc to improve mung bean yield and zinc-grains biofortification. Phyton-International Journal of Experimental Botany 89, 87–96.
Basal application of zinc to improve mung bean yield and zinc-grains biofortification.Crossref | GoogleScholarGoogle Scholar |

Khampuang K, Lordkaew S, Dell B, Prom-u-thai C (2021) Foliar zinc application improved grain zinc accumulation and bioavailable zinc in unpolished and polished rice. Plant Production Science 24, 94–102.
Foliar zinc application improved grain zinc accumulation and bioavailable zinc in unpolished and polished rice.Crossref | GoogleScholarGoogle Scholar |

Khan AM, Hussain S, Rengel Z, Shah MAA (2018) Zinc bioavailability and nitrogen concentration in grains of wheat crop sprayed with zinc sulfate, ammonium sulfate, ammonium chloride, and urea. Journal of Plant Nutrition 41, 1926–1936.
Zinc bioavailability and nitrogen concentration in grains of wheat crop sprayed with zinc sulfate, ammonium sulfate, ammonium chloride, and urea.Crossref | GoogleScholarGoogle Scholar |

Kihara J, Bolo P, Kinyua M, Rurinda J, Piikki K (2020) Micronutrient deficiencies in African soils and the human nutritional nexus: opportunities with staple crops. Environmental Geochemistry and Health 42, 3015–3033.
Micronutrient deficiencies in African soils and the human nutritional nexus: opportunities with staple crops.Crossref | GoogleScholarGoogle Scholar | 31902042PubMed |

Kiran A, Wakeel A, Mahmood K, Mubaraka R Kiran A, Wakeel A, Mahmood K, Mubaraka R (2022) Biofortification of staple crops to alleviate human malnutrition: contributions and potential in developing countries. Agronomy 12, 452
Biofortification of staple crops to alleviate human malnutrition: contributions and potential in developing countries.Crossref | GoogleScholarGoogle Scholar |

Kisetu E, Lily S, Temba WJ, Rwebangila AP (2014) Effect of nitrogen and phosphorus on performance of maize planted at the varying soil depths. International Journal of Agricultural Policy and Research 2, 90–97.

Klikocka H, Marks M (2018) Sulphur and nitrogen fertilization as a potential means of agronomic biofortification to improve the content and uptake of microelements in spring wheat grain DM. Journal of Chemistry 2018, 9326820
Sulphur and nitrogen fertilization as a potential means of agronomic biofortification to improve the content and uptake of microelements in spring wheat grain DM.Crossref | GoogleScholarGoogle Scholar |

Kumar D, Uppal RS, Dhaliwal SS, Ram H (2016) Enrichment of rice grains with nitrogen, zinc and iron ferti-fortification. Indian Journal of Fertilisers 12, 52–57.

Kutman UB, Yildiz B, Ozturk L, Cakmak I (2010) Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen. Cereal Chemistry 87, 1–9.
Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen.Crossref | GoogleScholarGoogle Scholar |

Kutman UB, Yildiz B, Cakmak I (2011a) Improved nitrogen status enhances zinc and iron concentrations both in the whole grain and the endosperm fraction of wheat. Journal of Cereal Science 53, 118–125.
Improved nitrogen status enhances zinc and iron concentrations both in the whole grain and the endosperm fraction of wheat.Crossref | GoogleScholarGoogle Scholar |

Kutman UB, Yildiz B, Cakmak I (2011b) Effect of nitrogen on uptake, remobilization and partitioning of zinc and iron throughout the development of durum wheat. Plant and Soil 342, 149–164.
Effect of nitrogen on uptake, remobilization and partitioning of zinc and iron throughout the development of durum wheat.Crossref | GoogleScholarGoogle Scholar |

Kutman UB, Kutman BY, Ceylan Y, Ova EA, Cakmak I (2012) Contributions of root uptake and remobilization to grain zinc accumulation in wheat depending on post-anthesis zinc availability and nitrogen nutrition. Plant and Soil 361, 177–187.
Contributions of root uptake and remobilization to grain zinc accumulation in wheat depending on post-anthesis zinc availability and nitrogen nutrition.Crossref | GoogleScholarGoogle Scholar |

Lateef EMAE, Tawfik MM, Hozyin M, Bakry BA, Elewa TA, Farrag AA, Bahr AA (2012) Soil and foliar fertilization of mungbean (Vigna radiata (L.) Wilczek) under Egyptian conditions. Elixir Agriculture 47, 8622–8628.

Li M, Wang S, Tian X, Li S, Chen Y, Jia Z, Liu K, Zhao A (2016) Zinc and iron concentrations in grain milling fractions through combined foliar applications of Zn and macronutrients. Field Crops Research 187, 135–141.
Zinc and iron concentrations in grain milling fractions through combined foliar applications of Zn and macronutrients.Crossref | GoogleScholarGoogle Scholar |

Li H, Lian C, Zhang Z, Shi X, Zhang Y (2017) Agro-biofortification of iron and zinc in edible portion of crops for the global south. Advances in Plants & Agriculture Research 6, 52–54.
Agro-biofortification of iron and zinc in edible portion of crops for the global south.Crossref | GoogleScholarGoogle Scholar |

Li M, Wang S, Tian X, Huang Y (2018) Improving nutritional quality of wheat grain through foliar zinc combined with macronutrients. Agronomy Journal 110, 38–46.
Improving nutritional quality of wheat grain through foliar zinc combined with macronutrients.Crossref | GoogleScholarGoogle Scholar |

Lone AH, Najar GR, Ganie MA (2017) Biofortification in rice grains vis-à-vis zinc and nitrogen fertilization. International Journal of Advances in Science Engineering and Technology 5, 18–21.

Manzeke MG, Mtambanengwe F, Watts MJ, Broadley MR, Lark RM, Mapfumo P (2020) Nitrogen effect on zinc biofortification of maize and cowpea in Zimbabwean smallholder farms. Agronomy Journal 112, 2256–2274.
Nitrogen effect on zinc biofortification of maize and cowpea in Zimbabwean smallholder farms.Crossref | GoogleScholarGoogle Scholar |

Manzeke-Kangara MG, Mtambanengwe F, Watts MJ, Broadley MR, Lark RM, Mapfumo P (2021) Can nitrogen fertilizer management improve grain iron concentration of agro-biofortified crops in Zimbabwe? Agronomy 11, 124
Can nitrogen fertilizer management improve grain iron concentration of agro-biofortified crops in Zimbabwe?Crossref | GoogleScholarGoogle Scholar |

Marques E, Darby HM, Kraft J (2021) Benefits and limitations of non-transgenic micronutrient biofortification approaches. Agronomy 11, 464
Benefits and limitations of non-transgenic micronutrient biofortification approaches.Crossref | GoogleScholarGoogle Scholar |

Medina-Lozano I, Díaz A (2022) Applications of genomic tools in plant breeding: crop biofortification. International Journal of Molecular Sciences 23, 3086
Applications of genomic tools in plant breeding: crop biofortification.Crossref | GoogleScholarGoogle Scholar | 35328507PubMed |

Mondal MMA, Rahman MA, Akter MB, Fakir MSA (2011) Effect of foliar application of nitrogen and micronutrients on growth and yield in mungbean. Legume Research 34, 166–171.

Montoya M, Vallejo A, Recio J, Guardia G, Alvarez JM (2020) Zinc–nitrogen interaction effect on wheat biofortification and nutrient use efficiency. Journal of Plant Nutrition and Soil Science 183, 169–179.
Zinc–nitrogen interaction effect on wheat biofortification and nutrient use efficiency.Crossref | GoogleScholarGoogle Scholar |

Mugenzi I, Yongli D, Ngnadong WA, Dan H, Niyigaba E, Twizerimana A, Jiangbo H (2018) Effect of combined zinc and iron application rates on summer maize yield, photosynthetic capacity and grain quality. International Journal of Agronomy and Agricultural Research 12, 36–46.

Mushtaq Z, Nazir A (2021) Biofortification: way forward toward micronutrient deficiency. International Journal of Environmental Quality 42, 36–41.
Biofortification: way forward toward micronutrient deficiency.Crossref | GoogleScholarGoogle Scholar |

Nandan B, Sharma BC, Chand G, Bazgalia K, Kumar R, Banotra M (2018) Agronomic fortification of Zn and Fe in chickpea an emerging tool for nutritional security – a global perspective. Acta Scientific Nutritional Health 2, 12–19.

Nemati AR, Sharifi RS (2012) Effects of rates and nitrogen application timing on yield, agronomic characteristics and nitrogen use efficiency in corn. International Journal of Agricultural and Crop Sciences 4, 534–539.

Nikhil K, Salakinkop SR (2018) Agronomic biofortification of maize with zinc and iron micronutrients. Modern Concepts & Developments in Agronomy 1, 87–90.
Agronomic biofortification of maize with zinc and iron micronutrients.Crossref | GoogleScholarGoogle Scholar |

Niyigaba E, Twizerimana A, Mugenzi I, Ngnadong WA, Ye YP, Wu BM, Hai JB (2019) Winter wheat grain quality, zinc and iron concentration affected by a combined foliar spray of zinc and iron fertilizers. Agronomy 9, 250
Winter wheat grain quality, zinc and iron concentration affected by a combined foliar spray of zinc and iron fertilizers.Crossref | GoogleScholarGoogle Scholar |

Pal V, Singh G, Dhaliwal SS (2019a) Agronomic biofortification of chickpea with zinc and iron through application of zinc and urea. Communications in Soil Science and Plant Analysis 50, 1864–1877.
Agronomic biofortification of chickpea with zinc and iron through application of zinc and urea.Crossref | GoogleScholarGoogle Scholar |

Pal V, Singh G, Dhaliwal SS (2019b) Yield enhancement and biofortification of chickpea (Cicer arietinum L.) grain with iron and zinc through foliar application of ferrous sulfate and urea. Journal of Plant Nutrition 42, 1789–1802.
Yield enhancement and biofortification of chickpea (Cicer arietinum L.) grain with iron and zinc through foliar application of ferrous sulfate and urea.Crossref | GoogleScholarGoogle Scholar |

Pal V, Singh G, Dhaliwal SS (2020) Symbiotic parameters, growth, productivity and profitability of chickpea as influenced by zinc sulphate and urea application. Journal of Soil Science and Plant Nutrition 20, 738–750.
Symbiotic parameters, growth, productivity and profitability of chickpea as influenced by zinc sulphate and urea application.Crossref | GoogleScholarGoogle Scholar |

Pal V, Singh G, Dhaliwal SS (2021) A new approach in agronomic biofortification for improving zinc and iron content in chickpea (Cicer arietinum L.) grain with simultaneous foliar application of zinc sulphate, ferrous sulphate and urea. Journal of Soil Science and Plant Nutrition 21, 883–896.
A new approach in agronomic biofortification for improving zinc and iron content in chickpea (Cicer arietinum L.) grain with simultaneous foliar application of zinc sulphate, ferrous sulphate and urea.Crossref | GoogleScholarGoogle Scholar |

Palmgren MG, Clemens S, Williams LE, Krämer U, Borg S, Schjørring JK, Sanders D (2008) Zinc biofortification of cereals: problems and solutions. Trends in Plant Science 13, 464–473.
Zinc biofortification of cereals: problems and solutions.Crossref | GoogleScholarGoogle Scholar | 18701340PubMed |

Pooja C, Sarawad IM (2019) Influence of iron and zinc on yield, quality of chickpea and status of iron and zinc in post harvest soil. Agricultural Science Digest 39, 31–35.
Influence of iron and zinc on yield, quality of chickpea and status of iron and zinc in post harvest soil.Crossref | GoogleScholarGoogle Scholar |

Potarzycki J, Grzebisz W (2009) Effect of zinc foliar application on grain yield of maize and its yielding components. Plant, Soil and Environment 55, 519–527.
Effect of zinc foliar application on grain yield of maize and its yielding components.Crossref | GoogleScholarGoogle Scholar |

Ram US, Srivastava VK, Hemantaranjan A, Sen A, Singh RK, Bohra JS, Shukla U (2013) Effect of Zn, Fe and FYM application on growth, yield and nutrient content of rice. Oryza 50, 351–357.

Rehman HU, Basra SMA, Farooq A (2011) Field appraisal of seed priming to improve the growth, yield and quality of direct seeded rice. Turkish Journal of Agricultural Research 35, 357–365.
Field appraisal of seed priming to improve the growth, yield and quality of direct seeded rice.Crossref | GoogleScholarGoogle Scholar |

Roman N, Zahida R, Kanth RH, Manzoor G, Shafeeq R, Ashaq H, Waseem R, Raies AB, Anwar M, Tahir S (2019) Agronomic biofortification of major cereals with zinc and iron – a review. Agricultural Reviews 40, 21–28.

Almad R, Rathod PS, Rachappa V, Dodamani BM, Ananda N (2020) Growth, yield and economics of pigeonpea as influenced by biofortification of zinc and iron. International Journal of Current Microbiology and Applied Sciences 9, 3088–3097.
Growth, yield and economics of pigeonpea as influenced by biofortification of zinc and iron.Crossref | GoogleScholarGoogle Scholar |

Saleem I, Javid S, Bibi F, Ehsan S, Niaz A, Ahmad ZA (2016) Biofortification of maize grains with zinc and iron by using fertilizing approach. Journal of Agriculture and Ecology Research International 7, 1–6.
Biofortification of maize grains with zinc and iron by using fertilizing approach.Crossref | GoogleScholarGoogle Scholar |

Saltzman A, Birol E, Oparinde A, Andersson MS, Asare-Marfo D, Diressie MT, Gonzalez C, Lividini K, Moursi M, Zeller M (2017) Availability, production and consumption of crops biofortified by plant breeding: current evidence and future potential. Annals of the New York Academy of Sciences 1390, 104–114.
Availability, production and consumption of crops biofortified by plant breeding: current evidence and future potential.Crossref | GoogleScholarGoogle Scholar | 28253441PubMed |

Shahane AA, Shivay YS (2022) Agronomic biofortification of crops: current research status and future needs. Indian Journal of Fertilisers 18, 164–179.

Sher A, Naveed K, Ahmad G, Khan A, Khan SM, Shah S (2020) Grain zinc and iron enrichment through foliar application augments wheat yield under varying nitrogen regimes. Pakistan Journal of Botany 52, 85–94.
Grain zinc and iron enrichment through foliar application augments wheat yield under varying nitrogen regimes.Crossref | GoogleScholarGoogle Scholar |

Sher A, Sarwar B, Sattar A, Ijaz M, Ul-Allah S, Hayat MT, Manaf A, Qayyum A, Zaheer A, Iqbal J, Askary AE, Gharib AF, Ismail KA, Elesawy BH (2022) Exogenous application of zinc sulphate at heading stage of wheat improves the yield and grain zinc biofortification. Agronomy 12, 734
Exogenous application of zinc sulphate at heading stage of wheat improves the yield and grain zinc biofortification.Crossref | GoogleScholarGoogle Scholar |

Shi R, Zhang Y, Chen X, Sun Q, Zhang F, Römheld V, Zou C (2010) Influence of long-term nitrogen fertilization on micronutrient density in grain of winter wheat (Triticum aestivum L.). Journal of Cereal Science 51, 165–170.
Influence of long-term nitrogen fertilization on micronutrient density in grain of winter wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar |

Shukla AK, Behera SK (2020) Biofortification for overcoming zinc and iron malnutrition in Indian population: current research status and way forward. Indian Journal of Fertilisers 16, 1262–1276.

Shivay YS, Prasad R (2012) Zinc-coated urea improves productivity and quality of basmati rice (Oryza sativa L.) under zinc stress conditions. Journal of Plant Nutrition 35, 928–951.
Zinc-coated urea improves productivity and quality of basmati rice (Oryza sativa L.) under zinc stress conditions.Crossref | GoogleScholarGoogle Scholar |

Shivay YS, Kumar D, Prasad R (2008) Effect of zinc-enriched urea on productivity, zinc uptake and efficiency of an aromatic rice–wheat cropping system. Nutrient Cycling in Agroecosystems 81, 229–243.
Effect of zinc-enriched urea on productivity, zinc uptake and efficiency of an aromatic rice–wheat cropping system.Crossref | GoogleScholarGoogle Scholar |

Singh D, Prasanna R (2020) Potential of microbes in the biofortification of Zn and Fe in dietary food grains. A review. Agronomy for Sustainable Development 40, 15
Potential of microbes in the biofortification of Zn and Fe in dietary food grains. A review.Crossref | GoogleScholarGoogle Scholar |

Singh U, Kumar N, Praharaj CS, Singh SS, Kumar L (2015) Ferti-fortification: an easy approach for nutritional enrichment of chickpea. The Ecoscan 9, 731–736.

Singh BR, Timsina YN, Lind OC, Cagno S, Janssens K (2018) Zinc and iron concentration as affected by nitrogen fertilization and their localization in wheat grain. Frontiers in Plant Science 9, 307
Zinc and iron concentration as affected by nitrogen fertilization and their localization in wheat grain.Crossref | GoogleScholarGoogle Scholar | 29593765PubMed |

Soni J, Kushwaha HS (2020) Effect of foliar spray of zinc and iron on productivity of mungbean [Vigna radiata (L.) Wilzeck]. Journal of Pharmacognosy and Photochemistry 9, 108–111.

Stangoulis JCR, Knez M (2022) Biofortification of major crop plants with iron and zinc – achievements and future directions. Plant and Soil
Biofortification of major crop plants with iron and zinc – achievements and future directions.Crossref | GoogleScholarGoogle Scholar |

Tuiwong P, Lordkaew S, Veeradittakit J, Jamjod S, Prom-u-thai C (2022) Seed priming and foliar application with nitrogen and zinc improve seedling growth, yield, and zinc accumulation in rice. Agriculture 12, 144
Seed priming and foliar application with nitrogen and zinc improve seedling growth, yield, and zinc accumulation in rice.Crossref | GoogleScholarGoogle Scholar |

Uauy C, Distelfeld A, Fahima T, Blechl A, Dubcovsky J (2006) A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science 314, 1298–1301.
A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat.Crossref | GoogleScholarGoogle Scholar | 17124321PubMed |

Wang S, Li M, Tian X, Li J, Li H, Ni Y, Zhao J, Chen Y, Guo C, Zhao A (2015) Foliar zinc, nitrogen, and phosphorus application effects on micronutrient concentrations in winter wheat. Agronomy Journal 107, 61–70.
Foliar zinc, nitrogen, and phosphorus application effects on micronutrient concentrations in winter wheat.Crossref | GoogleScholarGoogle Scholar |

Wang S, Sun N, Yang S, Tian X, Liu Q (2021) The effectiveness of foliar applications of different zinc source and urea to increase grain zinc of wheat grown under reduced soil nitrogen supply. Journal of Plant Nutrition 44, 644–659.
The effectiveness of foliar applications of different zinc source and urea to increase grain zinc of wheat grown under reduced soil nitrogen supply.Crossref | GoogleScholarGoogle Scholar |

Waters BM, Uauy C, Dubcovsky J, Grusak MA (2009) Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain. Journal of Experimental Botany 60, 4263–4274.
Wheat (Triticum aestivum) NAM proteins regulate the translocation of iron, zinc, and nitrogen compounds from vegetative tissues to grain.Crossref | GoogleScholarGoogle Scholar | 19858116PubMed |

Xue Y-F, Yue S-C, Zhang Y-Q, Cui Z-L, Chen X-P, Yang F-C, Cakmak I, McGrath S-P, Zhang F-S, Zou C-Q (2012) Grain and shoot zinc accumulation in winter wheat affected by nitrogen management. Plant and Soil 361, 153–163.
Grain and shoot zinc accumulation in winter wheat affected by nitrogen management.Crossref | GoogleScholarGoogle Scholar |

Yadav RC, Sharma SK, Varma A, Rajawat MVS, Khan MS, Sharma PK, Malviya D, Singh UB, Rai JP, Saxena AK (2022) Modulation in biofertilization and biofortification of wheat crop by inoculation of zinc-solubilizing rhizobacteria. Frontiers in Plant Science 13, 777771
Modulation in biofertilization and biofortification of wheat crop by inoculation of zinc-solubilizing rhizobacteria.Crossref | GoogleScholarGoogle Scholar | 35283872PubMed |

Yagmur M, Arpali D, Gulser F (2017) Effects of zinc and urea as foliar application on nutritional properties and grain yield in barley (Hordeum vulgare L. Conv. Distichon) under semi arid condition. Fresenius Environmental Bulletin 26, 6085–6092.

Zhang J, Wu LH, Wang MY (2008) Iron and zinc biofortification in polished rice and accumulation in rice plant (Oryza sativa L.) as affected by nitrogen fertilization. Acta Agriculture Scandinavica, Section B – Soil & Plant Science 58, 267–272.
Iron and zinc biofortification in polished rice and accumulation in rice plant (Oryza sativa L.) as affected by nitrogen fertilization.Crossref | GoogleScholarGoogle Scholar |

Zhang Y, Shi R, Rezaul KM, Zhang F, Zou C (2010) Iron and zinc concentrations in grain and flour of winter wheat as affected by foliar application. Journal of Agricultural and Food Chemistry 58, 12268–12274.
Iron and zinc concentrations in grain and flour of winter wheat as affected by foliar application.Crossref | GoogleScholarGoogle Scholar | 21073194PubMed |

Zhang P, Ma G, Wang C, Zhu Y, Guo T (2019) Mineral elements bioavailability in milling fractions of wheat grain response to zinc and nitrogen application. Agronomy Journal 111, 2504–2511.
Mineral elements bioavailability in milling fractions of wheat grain response to zinc and nitrogen application.Crossref | GoogleScholarGoogle Scholar |

Zulfiqar U, Hussain S, Maqsood M, Ishfaq M, Ali N (2021) Zinc nutrition to enhance rice productivity, zinc use efficiency, and grain biofortification under different production systems. Crop Science 61, 739–749.
Zinc nutrition to enhance rice productivity, zinc use efficiency, and grain biofortification under different production systems.Crossref | GoogleScholarGoogle Scholar |