Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Identification and mapping of a stripe rust resistance gene in spring wheat germplasm HRMSN-81 from CIMMYT

Shi-Sheng Chen A D , Guo-Yue Chen A D , Cheng Yang A , Yu-Ming Wei A , Wen-Xiong Wu B , Yuan-Jiang He B , Ya-Xi Liu B , Wei Li C , Zhi-En Pu C , Xiu-Jin Lan A and You-Liang Zheng A B E
+ Author Affiliations
- Author Affiliations

A Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, P. R. China.

B Key Laboratory of Crop Germplasm Resources Utilisation in Southwest China, Ministry of Agriculture, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, P. R. China.

C College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan 611130, P. R. China.

D Shi-Sheng Chen and Guo-Yue Chen contributed equally to this paper.

E Corresponding author. Email: ylzheng@sicau.edu.cn

Crop and Pasture Science 64(1) 1-8 https://doi.org/10.1071/CP12393
Submitted: 22 November 2012  Accepted: 18 March 2013   Published: 16 April 2013

Abstract

Pathogens are a reason for low yield in common wheat (Triticum aestivum L.). Stripe rust (or yellow rust), caused by the fungus Puccinia striiformis f. sp. tritici (Pst), is one of the most important foliar diseases of wheat. One of the most cost-effective and environmentally sound ways to control stripe rust is to use plant varieties that are resistant to this pathogen. It is an important task for wheat breeders and pathologists to identify new genes and pyramid them in order to achieve high-level, durable resistance to stripe rust. One spring wheat germplasm, HRMSN-81, with resistance to the most dominant races in China, is identified from the CIMMYT breeding lines. To elucidate the genetic basis of its resistance, HRMSN-81 was crossed with susceptible wheat genotype Taichung 29. Seedlings of the parents were tested with Chinese Pst isolates CYR31, CYR32, and CYR33 under controlled greenhouse conditions, and adult plants of the parents and F1, F2, and F2:3 progeny were inoculated with the epidemic stripe rust mixed races, including CYR31, CYR32, and CYR33, in fields under natural infection.

Genetic analysis showed that HRMSN-81 has a single dominant gene conferring all-stage resistance, temporarily designated as YrHRMSN-81. Resistance gene analogue polymorphism (RGAP), simple sequence repeat (SSR), target region amplified polymorphism (TRAP), and sequence-related amplified polymorphism (SRAP) techniques in combination with bulked segregant analysis (BSA) were used to identify molecular markers linked to the resistance gene. A linkage map consisting of six RGAP, two SSR, one TRAP, and two SRAP markers was constructed for YrHRMSN-81 using 148 F2 plants. The gene was mapped to chromosome arm 2DS by testing the complete set of nulli-tetrasomic lines and selected ditelosomic lines with two RGAP markers and was further confirmed by two chromosome-specific SSR markers. The results of gene characteristics and chromosome locations indicated that YrHRMSN-81 was probably a new stripe rust resistance gene. The two flanking markers Xwgp-180bp (93% polymorphism rate) and Xwmc453 (91% polymorphism rate) detected 100% polymorphism of the 56 tested wheat genotypes when they were used in combination. The identification of the gene YrHRMSN-81 and the determination of the flanking markers should be useful for rapidly transferring it in wheat breeding programs.

Additional keywords: resistant gene, resistance gene analog polymorphism (RGAP), simple sequence repeat (SSR), wheat stripe rust.


References

Bariana HS, Hayden MJ, Ahmed NU, Bell JA, Sharp PJ, McIntosh RA (2001) Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat. Australian Journal of Agricultural Research 52, 1247–1255.
Mapping of durable adult plant and seedling resistances to stripe rust and stem rust diseases in wheat.CrossRef | 1:CAS:528:DC%2BD38XltlOmsg%3D%3D&md5=3744de0d68f0fd112875634cffe38b12CAS |

Bariana HS, Parry N, Barclay IR, Loughman R, McLean RJ, Shankar M, Wilson RE, Willey NJ, Francki M (2006) Identification and characterization of stripe rust resistance gene Yr34 in common wheat. Theoretical and Applied Genetics 112, 1143–1148.
Identification and characterization of stripe rust resistance gene Yr34 in common wheat.CrossRef | 1:CAS:528:DC%2BD28XjtVSmt78%3D&md5=77d37cdc83bc9e1ac99a3e13bce70ff3CAS | 16435125PubMed |

Chen XM (2005) Epidemiology and control of stripe rust on wheat. Canadian Journal of Plant Pathology 27, 314–337.
Epidemiology and control of stripe rust on wheat.CrossRef |

Chen XM (2007) Challenges and solutions for stripe rust control in the United States. Australian Journal of Agricultural Research 58, 648–655.
Challenges and solutions for stripe rust control in the United States.CrossRef |

Chen XM, Line RF (1992a) Identification of stripe rust resistance genes in wheat genotypes used to differentiate North American races of Puccinia striiformis. Phytopathology 82, 1428–1434.
Identification of stripe rust resistance genes in wheat genotypes used to differentiate North American races of Puccinia striiformis.CrossRef |

Chen XM, Line RF (1992b) Inheritance of stripe rust resistance in wheat cultivars used to differentiate races of Puccinia striiformis in North America. Phytopathology 82, 633–637.
Inheritance of stripe rust resistance in wheat cultivars used to differentiate races of Puccinia striiformis in North America.CrossRef |

Chen XM, Zhao J (2007) Identification of molecular markers for Yr8 and a gene for high-temperature, adult-plant resistance against stripe rust in the AVS/6*Yr8 wheat line. American Phytopathological Society Abstracts 97, S21

Chen XM, Moore M, Milus EA, Long DL, Line RF, Marshall D, Jackson L (2002) Wheat stripe rust epidemics and races of Puccinia striiformis f. sp. tritici in the United States in 2000. Plant Disease 86, 39–46.
Wheat stripe rust epidemics and races of Puccinia striiformis f. sp. tritici in the United States in 2000.CrossRef |

Cheng P (2008) Molecular mapping of a gene for resistance to stripe rust in spring wheat cultivar IDO377 s and identification of a new race of Puccinia striiformis f. sp. tritici virulent on IDO377s. MS Thesis, Washington State University, Washington DC, USA.

Cheng P, Chen XM (2010) Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s. Theoretical and Applied Genetics 121, 195–204.
Molecular mapping of a gene for stripe rust resistance in spring wheat cultivar IDO377s.CrossRef | 1:CAS:528:DC%2BC3cXmtFagu7g%3D&md5=5e4933e78706fc6b91d660825f26572bCAS | 20198466PubMed |

Fu DL, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen XM, Sela H, Fahima T, Dubcovsky J (2009) A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science 323, 1357–1360.
A kinase-START gene confers temperature-dependent resistance to wheat stripe rust.CrossRef | 1:CAS:528:DC%2BD1MXisFemtb0%3D&md5=7b8fd4cb914045c0e092f848ef2ba157CAS |

Herrera-Foessel SA, Lagudah ES, Huerta-Espino J, Hayden M, Bariana HS, Singh D, Singh RP (2011) New slow rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theoretical and Applied Genetics 122, 239–249.
New slow rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked.CrossRef | 20848270PubMed |

Hu J, Vick BA (2003) TRAP (target region amplification polymorphism), a novel marker technique for plant genotyping. Plant Molecular Biology Reporter 21, 289–294.
TRAP (target region amplification polymorphism), a novel marker technique for plant genotyping.CrossRef | 1:CAS:528:DC%2BD2cXhtVylu7s%3D&md5=2c2f59f22927388e3f15523c3a34d944CAS |

Huang X, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: A review. Euphytica 137, 203–223.
Molecular mapping of powdery mildew resistance genes in wheat: A review.CrossRef | 1:CAS:528:DC%2BD2cXnsFOrs7k%3D&md5=3daf6cb9636dd147064648aa4e6cc192CAS |

Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323, 1360–1363.
A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat.CrossRef | 1:CAS:528:DC%2BD1MXisFemtbg%3D&md5=3f8bcd1bc7031c44e1c07f68215fc220CAS | 19229000PubMed |

Lagudah ES (2011) Molecular genetics of race non-specific rust resistance in wheat. Euphytica 179, 81–91.
Molecular genetics of race non-specific rust resistance in wheat.CrossRef |

Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.
MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.CrossRef | 1:CAS:528:DyaL1cXhsVCksrk%3D&md5=6820e32770be21e6bb77a221394222c3CAS | 3692487PubMed |

Li ZQ, Zeng SM (2000) ‘Wheat rusts in China.’ (China Agricultural Press: China)

Li Z, Li B, Tong Y (2008) The contribution of distant hybridization with decaploid Agropyron elongatum to wheat improvement in China. Journal of Genetics and Genomics 35, 451–456.
The contribution of distant hybridization with decaploid Agropyron elongatum to wheat improvement in China.CrossRef | 18721781PubMed |

Li AX, Liu QC, Wang QM, Zhang LM, Zhai H, Liu SZ (2010) Construction of molecular linkage maps using SRAP markers in sweetpotato. Acta Agronomica Sinica 36, 1286–1295.
Construction of molecular linkage maps using SRAP markers in sweetpotato.CrossRef | 1:CAS:528:DC%2BC3cXhsVWitb%2FI&md5=6b7e5fb31d4a1662a8bdbbaf98806817CAS |

Li Q, Chen XM, Wang MN, Jing JX (2011) Yr45, a new wheat gene for stripe rust resistance mapped on the long arm of chromosome 3D. Theoretical and Applied Genetics 122, 189–197.
Yr45, a new wheat gene for stripe rust resistance mapped on the long arm of chromosome 3D.CrossRef | 1:STN:280:DC%2BC3M%2Fns1CjsA%3D%3D&md5=0706fe945e8f9f7c5e9d7a925c558134CAS | 20838759PubMed |

Line RF (2002) Stripe rust of wheat and barley in North America: a retrospective historical review. Annual Review of Phytopathology 40, 75–118.
Stripe rust of wheat and barley in North America: a retrospective historical review.CrossRef | 1:CAS:528:DC%2BD38Xos1CltLk%3D&md5=3b5b99a8979671297a0880e16d2b6271CAS | 12147755PubMed |

Liu XK (1988) Study on the yellow rust resistance to common wheat (T. aestivum). Plant Protect 15, 33–39.

Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Heraditas 3, 317–321.

Lowe L, Jankuloski L, Chao S, Chen XM, See D, Dubcovsky J (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat. Theoretical and Applied Genetics 123, 143–157.
Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat.CrossRef |

Marais GF, McCallum B, Snyman JE, Pretorius ZA, Marais AS (2005) Leaf rust and stripe rust resistance genes Lr54 and Yr37 transferred to wheat from Aegilops kotschyi. Plant Breeding 124, 538–541.
Leaf rust and stripe rust resistance genes Lr54 and Yr37 transferred to wheat from Aegilops kotschyi.CrossRef | 1:CAS:528:DC%2BD28Xjs1arsw%3D%3D&md5=029c0e2fad6967f0a8ed09e93c7a3ec6CAS |

McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Somers DJ, Appels R, Devos KM (2008) Catalogue of gene symbols for wheat. In ‘Proceedings of 11th International Wheat Genetics Symposium’. 24–29 August 2008, Brisbane, Qld. (Eds R Appels et al.) (Sydney University Press: Sydney) Available at: http://wheat.pw.usda.gov/GG2/Triticum/wgc/2008/GeneSymbol.pdf

McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2009) Catalogue of gene symbols for wheat: 2009 supplement. Available at: www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2009.pdf

McIntosh RA, Dubcovsk J, Rogers WJ, Morris C, Appels R, Xia XC (2010) Catalogue of gene symbols for wheat: 2010 supplement. Annual Wheat Newsletter 56, 273–282.

Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregation populations. Proceedings of the National Academy of Sciences of the United States of America 88, 9828–9832.
Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregation populations.CrossRef | 1:CAS:528:DyaK38Xhs1emtw%3D%3D&md5=bd5d26b88783262230cdf23f85a23636CAS | 1682921PubMed |

Navabi A, Tewari JP, Singh RP, McCallum B, Laroche A, Briggs KG (2005) Inheritance and QTL analysis of durable resistance to stripe and leaf rusts in an Australian cultivar, Triticum aestivum ‘Cook’. Genome 48, 97–107.
Inheritance and QTL analysis of durable resistance to stripe and leaf rusts in an Australian cultivar, Triticum aestivum ‘Cook’.CrossRef | 1:CAS:528:DC%2BD2MXjs1Wmu7s%3D&md5=25074944913b3ce2a6245c1a72011e99CAS | 15729401PubMed |

Ren RS, Wang MN, Chen XM, Zhang ZJ (2012) Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527. Theoretical and Applied Genetics 125, 847–857.
Characterization and molecular mapping of Yr52 for high-temperature adult-plant resistance to stripe rust in spring wheat germplasm PI 183527.CrossRef | 1:CAS:528:DC%2BC38Xht1ajt7%2FP&md5=e0c3493f462b2711ebf3d462f5624132CAS | 22562146PubMed |

Riley R, Chapman V, Johnson R (1968) The incorporation of alien disease resistance in wheat by genetic interference with the regulation of meiotic chromosome synapsis. Genetical Research, Cambridge 12, 199–219.
The incorporation of alien disease resistance in wheat by genetic interference with the regulation of meiotic chromosome synapsis.CrossRef |

Saghai MA, Biyashev RM, Yang GP, Zhang Q, Allard RW (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics. Proceedings of the National Academy of Sciences of the United States of America 91, 5466–5470.
Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations, and population dynamics.CrossRef |

Shi ZX, Chen XM, Line RF, Leung H, Wellings CR (2001) Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome 44, 509–516.

Sui XX, Wang MN, Chen XM (2009) Molecular mapping of a stripe rust resistance gene in spring wheat genotype ‘Zak’. Phytopathology 99, 1209–1215.
Molecular mapping of a stripe rust resistance gene in spring wheat genotype ‘Zak’.CrossRef | 1:CAS:528:DC%2BD1MXht1OqtbzN&md5=d777ff2904fb7e4f4dad826c9e334435CAS | 19740035PubMed |

Wan AM, Zhao ZH, Chen XM, He ZH, Jin SL, Jia QZ, Yao G, Yang JX, Wang BT, Li GB, Bi YQ, Yuan ZY (2004) Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002. Plant Disease 88, 896–904.
Wheat stripe rust epidemic and virulence of Puccinia striiformis f. sp. tritici in China in 2002.CrossRef |

Wan AM, Chen XM, He ZH (2007) Wheat stripe rust in China. Australian Journal of Agricultural Research 58, 605–619.
Wheat stripe rust in China.CrossRef |

Wellings CR, Wright DG, Keiper F, Loughman R (2003) First detection of wheat stripe rust in Western Australia: evidence for a foreign incursion. Australasian Plant Pathology 32, 321–322.
First detection of wheat stripe rust in Western Australia: evidence for a foreign incursion.CrossRef |

Worland AJ, Law CN (1986) Genetic analysis of chromosome 2D of wheat I: The location of genes affecting height, day-length insensitivity, hybrid dwarfism and yellow-rust resistance. Zeitschrift fur Pflanzenzuchtung 96, 331–345.

Worland AJ, Petrovic S, Law CN (1988) Genetic analysis of chromosome 2D of wheat II: The importance of this chromosome to Yugoslavian varieties. Plant Breeding 100, 247–259.
Genetic analysis of chromosome 2D of wheat II: The importance of this chromosome to Yugoslavian varieties.CrossRef |



Rent Article (via Deepdyve) Export Citation Cited By (1)