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Plant sciences, sustainable farming systems and food quality

Estimating genetic diversity in Greek durum wheat landraces with RAPD markers

Anna Mantzavinou A , Penelope J. Bebeli A B and Pantouses J. Kaltsikes A

A Department of Plant Breeding and Biometry, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.

B Corresponding author. Email:

Australian Journal of Agricultural Research 56(12) 1355-1364
Submitted: 20 October 2004  Accepted: 17 August 2005   Published: 15 December 2005


Using the random amplified polymorphic DNA (RAPD) method, the genetic diversity of 19 Greek landraces and 9 cultivars of durum wheat [Triticum turgidum L. var. durum (Desf.)] was studied. Two commercial bread wheat (Triticum aestivum L.) cultivars and one genotype of Triticum monococcum L. were also included in the study. Eighty-seven arbitrary primers (10-mer) were evaluated in a preliminary experiment and 15 of them were selected for the main experiments based on the quality and reliability of their amplification and the polymorphism they revealed. A total of 150 DNA bands were obtained, 125 (83.3%) of which were polymorphic. On average, 10 DNA bands were amplified per primer, 8.3 of which were polymorphic. The genetic similarity between all pairs of genotypes was evaluated using the Jaccard’s or Nei and Li’s coefficients; the values of the former ranged from 0.153 to 0.973 while those of the latter were slightly higher (0.265–0.986). Cluster analysis was conducted by the UPGMA and the Njoin methods. Both methods broadly placed 26 durum genotypes into 1 branch while the other branch consisted of 2 subgroups: 1 included the 2 bread wheat cultivars; the other 1 consisted of 2 durum landraces, ‘Kontopouli’ and ‘Mavrotheri-Chios’, which showed an intruiging behaviour sharing bands with the bread wheat cultivars. The T. monococcum cultivar stood apart from all other genotypes.

Additional keywords: biodiversity, dendrogram, DNA markers, genetic relationships, germplasm, plant genetic resources.


Autrique E Nachit MM Monneveux P Tanksley SD Sorrells ME 1996 Genetic diversity in durum wheat based on RFLPs, morphophysiological traits and coefficient of parentage. Crop Science 36 735 742

Barrett BA Kidwell KK Fox PN 1998 Comparison of AFLP and pedigree-based genetic diversity assessment methods using wheat cultivars from the Pacific Northwest. Crop Science 38 1271 1278

Biesantz A Limberg P Kyzeridis N 1990 Evaluation of Greek and Turkish durum wheat landraces. ‘Wheat genetic resources. Meeting diverse needs’. Srivastava JP Damania AB 45 56 John Wiley and Sons Chichester, UK

Boggini G Palumbo M Galcagno F 1990 Characterization and utilization of Sicilian landraces of durum wheat in breeding programmes. ‘Wheat genetic resources. Meeting diverse needs’. Srivastava JP Damania AB 223 234 John Wiley and Sons Chichester, UK

Brown AHD Marshall DR 1995 A basic sampling strategy: theory and practice. ‘Collecting plant genetic diversity technical guidelines’. Guarino L. Ramanatha Rao V Reid R 75 91 CAB International Wallingford, UK

Cao W Scoles G Huci P Chibbar RN 2000 Phylogenetic relationships of five morphological groups of hexaploid wheat (Triticum aestivum L. em Thell.) based on RAPD analysis. Genome 43 724 727

Castagna R Gnocchi S Perenzin M Heum M 1997 Genetic variability of the wild diploid wheat Triticum uratru revealed by RFLP and RAPD markers. Theoretical and Applied Genetics 94 424 430 doi:10.1007/s001220050432

Damania AB Porceddu E Jackson MT 1983 A rapid method for evaluation of variation in germplasm collections of cereals using polyacrylamide gel electrophoresis. Euphytica 32 877 883 doi:10.1007/BF00042169

Dencic S Kastori R Kobiljski B Duggan B 2000 Evaluation of grain yield and its components in wheat cultivars and landraces under near optimal and drought conditions. Euphytica 113 43 52

Dograr N Akin-Yalin S Akkaya MS 2000 Discriminating durum wheat cultivars using highly polymorphic simple sequence repeat DNA markers. Plant Breeding 119 360 362

Esquinas-Alcazar JT 1993 Plant genetic resources. ‘Plant breeding. Principles and prospects’. Hayward MD Bosemark NO Romagosa I 33 51 Cambridge University Press Cambridge, UK

Fischbeck G 1989 Evaluation of cereals in Europe. ‘The use of plant genetic resources’. Brown AHD Frankel OH Marshall DR Williams JT 197 211 Cambridge University Press Cambridge, UK

Jaradat AA 1991 Phenotypic divergence for morphological and yield-related traits among landrace genotypes of durum wheat from Jordan. Euphytica 52 155 164

Johns MA Scrotch PW Nienhuis I Hinrichsen P Bascur G Munoz-Schick C 1997 Gene pool classification of common bean landraces from Chile based on RAPD and morphological data. Crop Science 37 605 613

Joshi CP Nguyen HT 1993 Application of the random amplified polymorphic DNA technique for the detection of polymorphism among wild and cultivated tetraploid wheats. Genome 36 602 609

Kudryavtsev AM Martynov SP Broggio M Pukhalskiy VA 2003 Relevance of RAPD analysis for revealing phylogenetic relationships between cultivars of durum wheat Triticum durum Desf. Russian Journal of Genetics 39 1043 1051

Kyzeridis N Biesantz A Limberg P 1995 Comparative trials with durum-wheat landraces and cultivars in different ecological environments in the Mediterranean region. Journal of Agronomy and Crop Science 174 133 144 (In German with an abstract in English)

Lamboy WF 1994 Computing genetic similarity coefficients from RAPD data: the effects of PCR artifacts. PCR Methods and Applications 4 31 37

Michelmore RW Paran I Kesseli RV 1991 Identification of markers linked to desease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregation populations. Proceedings of the National Academy of Sciences of the USA 88 9828 9832

Mukhtar MS Rahman M Zafar Y 2002 Assessment diversity among wheat (Triticum aestivum L.) cultivars from a range of localities across Pakistan using random amplified polymorphic DNA (RAPD) analysis. Euphytica 128 417 425

Nagaoka T Ogihara Y 1997 Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theoretical and Applied Genetics 94 597 602 doi:10.1007/s001220050456

Nachit MM Ketata H Yau SK 1988 Breeding durum wheat for stress environments of the Mediterranean region. ‘Proceedings of the 3rd International Symposium on Durum Wheat. The future of cereals for human feeding and development of biotechnological research’. Wittner G 297 304 Chamber of Commerce Foggia, Italy

Nei M Li WH 1979 Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the USA 76 5269 5273

Pecetti L Doust MA Calcagno L Raciti CN Boggini G 2001 Variation of morphological and agronomical traits, and protein composition in durum wheat germplasm from eastern Europe. Genetic Resources and Crop Evolution 48 609 620

Pujar S Tamhankar SA Rao VS Gupta VS Naik S Ranjekar PK 1999 Arbitrarily primed PCR based diversity assessment reflects hierarchical groupings of Indian tetraploid wheat genotypes. Theoretical and Applied Genetics 99 868 876 doi:10.1007/s001220051307

Rohlf FJ 1998 ‘NTSYS – Numerical taxonomy and multivariate analysis system.’ Exeter Publications New York

Saghai-Maroof MA Soliman KM Jorgensen RA Allard RW 1984 Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proceedings of the National Academy of Sciences of the USA 81 8014 8018

Saitu N Nei M 1987 The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4 406 425

Schaffer HE Sederoff RR 1981 DNA fragments. Analytical Biochemistry 115 113 122

Sneath PHA Sokal RR 1973 ‘Numerical taxonomy.’ WH Freeman and Company San Francisco, CA

Soleimani VD Baum BR Johnson DA 2002 AFLP and pedigree-based genetic diversity estimates in modern cultivars of durum wheat [Triticum turgidum L. subsp. durum (Desf.) Husn.]. Theoretical and Applied Genetics 104 350 357

Sun Q Ni Z Liu Z Cao J Huang T 1998 Genetic relationships and diversity among Tibetan wheat, common wheat and European spelt wheat revealed by RAPD markers. Euphytica 99 205 211 doi:10.1023/A:1018316129246

Sun G Bond M Nass H Martin R Dong Z 2003 RAPD polymorhisms in spring wheat cultivars and lines with different level of Fusarium resistance. Theoretical and Applied Genetics 106 1059 1067

Vierling RA Nguyen HT 1992 Use of RAPD markers to determine the genetic diversity of diploid wheat genotypes. Theoretical and Applied Genetics 84 835 838

Williams JGK Kubelik AR Livak KJ Rafalski JA Tingley SV 1990 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18 6531 6535

Yang X Quiros C 1993 Identification and classification of celery cultivars with RAPD markers. Theoretical and Applied Genetics 86 205 212

Zeven AC Zeven-Hissink NC 1976 ‘Genealogies of 14 000 wheat varieties.’ The Netherlands Cereals Center-NGC, Wageningen and The International Maize and Wheat Improvement Center-CIMMYT Mexico

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