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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 61(8)

Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH

Friederike S. Eberhard A, Peng Zhang B, Anke Lehmensiek A, Ray A. Hare C, Steven Simpfendorfer C, Mark W. Sutherland A D

A Centre for Systems Biology, University of Southern Queensland, Toowoomba, Qld 4350, Australia.
B University of Sydney, Plant Breeding Institute, Cobbitty, NSW 2570, Australia.
C New South Wales Industry and Investment, Tamworth Agricultural Institute, Tamworth, NSW 2340, Australia.
D Corresponding author. Email: mark.sutherland@usq.edu.au
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This study has employed multicolour fluorescence in situ hybridisation (MCFISH) and Diversity Arrays Technology (DArT) markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat [T. turgidum L. spp. durum (Desf.) var. Bellaroi]. In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis carried some D genome material, principally as entire chromosomes, while 40 plants included at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.

Keywords: DArT, durum wheat, hexaploid × durum crosses, MCFISH.

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