CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Functional Plant Biology   
Functional Plant Biology
Journal Banner
  Plant Function & Evolutionary Biology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Reviews
Evolutionary Reviews
Sample Issue
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review an Article
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow PrometheusWiki
blank image
PrometheusWiki
Protocols in ecological and environmental plant physiology

 

Article << Previous     |     Next >>   Contents Vol 29(11)

Plant growth regulators and phloem exudates modulate root nodulation of soybean

Asghari Bano and James E. Harper

Functional Plant Biology 29(11) 1299 - 1307
Published: 25 November 2002

Abstract

A time-course study was conducted on the effect of synthetic ABA and phloem exudate on root nodulation, nitrogenase activity, and dry matter content of pouch-grown soybean [Glycine max (L.) Merr.]. A hypernodulating mutant (NOD1-3) derived from the cultivar Williams was evaluated. The normally-nodulating cultivar Williams 82 served as the control. All phases of nodulation (nodule initiation, development and function) were inhibited by 1–50 μM synthetic ABA treatment of roots of pouch-grown soybean. The ABA effect was more pronounced for the treatment made 24 h prior to inoculation. The magnitude of inhibition of nodulation by ABA and by phloem exudate from inoculated Williams 82 was decreased when application was delayed to 48 h post-inoculation. Synthetic indoleacetic acid and benzyladenine (1 and 5 μM) also inhibited the number of nodules per plant, and the magnitude of inhibition was greater in the normally-nodulating Williams 82 cultivar than in the NOD1-3 hypernodulating mutant. Changes in endogenous free ABA in cotyledons, leaves, and xylem sap of seedlings were monitored at 24-h intervals after inoculation in both lines, and appeared to respond to inoculation. Addition of crude phloem exudate from Williams 82 or hypernodulating NOD1-3 was inhibitory to root nodulation of NOD1-3 — this effect was concentration dependent. Partially-purified (acidic aqueous fraction) phloem exudate from Williams 82 suppressed nodulation in NOD1-3 by 54% (averaged over a 7-d time-course), while partially-purified phloem exudate (acidic aqueous fraction) from NOD1-3 was much less inhibitory (23%). ABA levels in the phloem fractions applied were nearly identical (15.6 vs 16.0 ng mL–1), which indicates that ABA was not directly affecting nodulation control. Partially-purified extract (acidic aqueous fraction) from leaves of nodulated NOD1-3 was much less inhibitory to nodulation of NOD1-3 seedlings than was the comparable fraction from Williams 82. Although free ABA applied exogenously was capable of altering nodulation, it does not appear that endogenous ABA levels directly account for the autoregulatory control of nodulation in soybean. This implies that there may be factor(s) moving in the phloem other than ABA that possibly impact expression of root nodulation. Alternatively, it may be that plant development is responsive to interactions among plant growth regulators mediated by subtle changes in individual hormone concentrations. The possibility remains that normally-nodulating and hypernodulating lines have differential sensitivity to ABA, and that this explains altered nodulation control at similar levels of endogenous ABA.

Keywords: ABA, abscisic acid, autoregulation, Glycine max, hypernodulation, nodulation events.



Full text doi:10.1071/FP02031

© CSIRO 2002

blank image
Subscriber Login
Username:
Password:  

 
PDF (241 KB) $25
 Export Citation
 Print
  
    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2015