CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Functional Plant Biology   
Functional Plant Biology
Journal Banner
  Plant Function & Evolutionary Biology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Reviews
Evolutionary Reviews
Sample Issue
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review Article
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow PrometheusWiki
blank image
PrometheusWiki
Protocols in ecological and environmental plant physiology

 

Article << Previous     |         Contents Vol 33(1)

Isolation of a NaCl-tolerant mutant of Chrysanthemum morifolium by gamma radiation: in vitro mutagenesis and selection by salt stress

Zahed Hossain A, Abul Kalam Azad Mandal A, Subodh Kumar Datta A C, Amal K. Biswas B

A Botanic Garden and Floriculture, National Botanical Research Institute, Lucknow 226 001, Uttar Pradesh, India.
B Cytogenetics and Plant Breeding Laboratory, Botany Department, University of Kalyani, Kalyani 741 235, West Bengal, India.
C Corresponding author. Email: subodhdatta@usa.net
 
PDF (362 KB) $25
 Export Citation
 Print
  


Abstract

A stable NaCl-tolerant mutant (R1) of Chrysanthemum morifolium Ramat has been developed by in vitro mutagenesis with gamma radiation (5 gray; Gy). Salt tolerance was evaluated by the capacity of the plant to maintain both flower quality and yield under NaCl stress. Enhanced salt tolerance of the R1 mutant was attributed to increased activities of reactive oxygen species (ROS)-scavenging enzymes, namely superoxide dismutase (SOD), monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), and to reduced membrane damage, higher relative water content (RWC), chlorophyll and carotenoids contents. RAPD analysis revealed two polymorphic bands (956 and 1093 bp) for the R1 mutant that might be considered as specific RAPD markers associated with salt tolerance. Better performance of the R1 progeny under identical salinity stress conditions, even in the second year, confirmed the genetic stability of the induced salt tolerance character. The R1 mutant developed by gamma ray treatment can be considered a salt-tolerant mutant showing all the positive characteristics of tolerance to NaCl stress.

Keywords: antioxidant enzymes, Chrysanthemum, gamma ray, in vitro mutagenesis, NaCl-tolerant, reactive oxygen species.


   
Subscriber Login
Username:
Password:  

    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014