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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 42(2)

Heterotrimeric G-proteins involved in the MeJA regulated ion flux and stomatal closure in Arabidopsis thaliana

Suli Yan A B *, Shuitian Luo A B *, Shanshan Dong A B, Ting Zhang A B, Jingru Sun A B, Ningning Wang A B, Hongjun Yao A and Yingbai Shen A B C

A College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100 083, China.
B National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100 083, China.
C Corresponding author. Email: ybshen@bjfu.edu.cn

Functional Plant Biology 42(2) 126-135 http://dx.doi.org/10.1071/FP14162
Submitted: 14 June 2014  Accepted: 15 September 2014   Published: 15 October 2014

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Heterotrimeric G-proteins play an important role in plant signalling pathways. The plant hormone methyl jasmonate (MeJA) can induce stomatal closure in many plant species. The signal cascade in MeJA-induced stomatal closure has been studied previously. However, the function of G proteins in this process has not yet been evaluated. In this study, the stomatal movement induced by MeJA in the wild-type Arabidopsis thaliana (L. Heynh.) (WS), Gα subunit loss-of-function mutant gpa11 and gpa12 guard cells were measured. Further, the transmembrane ion flux (H+, Ca2+ and K+) and reactive oxygen species (ROS) experiments were performed in guard cells from WS, GDP-β-S pre-treated WS, gpa11 and gpa12 using non-invasive micro-test technique (NMT) and confocal technique. It was observed that the MeJA-induced stomatal closure was abolished in guard cells of gpa1 mutants. GDP-β-S pre-treatment and gpa1 mutants impaired the MeJA-activated H+ efflux, Ca2+ influx and K+ efflux. The accumulation of ROS in gpa11 and gpa12 guard cells was also lower than that in WS guard cells under MeJA treatment. These results suggested that Gα subunits are involved in regulating the signal events in JA signal pathway and stomatal closure.

Additional keywords: guard cell, heterotrimeric G-proteins, ion flux, MeJA.


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