CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Functional Plant Biology   
Functional Plant Biology
Journal Banner
  Plant Function & Evolutionary Biology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Reviews
Evolutionary Reviews
Sample Issue
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review an Article
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow PrometheusWiki
blank image
PrometheusWiki
Protocols in ecological and environmental plant physiology

 

Article << Previous     |     Next >>   Contents Vol 26(3)

Auxin induction is a trigger for root gall formation caused by root-knot nematodes in white clover and is associated with the activation of the flavonoid pathway

Pokkwan Hutangura, Ulrike Mathesius, Mike G. K. Jones and Barry G. Rolfe

Australian Journal of Plant Physiology 26(3) 221 - 231
Published: 1999

Abstract

We studied the expression of the auxin responsive promoter (GH3) fused to the gusA reporter gene in white clover (Trifolium repens cv. Haifa) during the initiation of root galls by root-knot nematodes (Meloidogyne javanica) to investigate whether nematode infection affects auxin distribution in developing galls. In search for a plant signal that would mediate changes in auxin location we studied the induction of the flavonoid pathway because flavonoids can act as auxin transport regulators. Three chalcone synthase (CHS1, CHS2 and CHS3) promoter:gusA fusions were examined in transgenic plants and flavonoids were detected using fluorescence microscopy. Within 24 h post inoculation CHS:gusA expression occurred around the invading nematode. At 48 h post inoculation CHS:gusA expression and flavonoids were detected throughout the infection site, followed by high GH3:gusA expression in the gall 48–72 h post inoculation. Initially (48–72 h post inoculation) high GH3:gusA expression in giant cell precursors was followed by low expression in the enlarging giant cells (96–120 h post inoculation), suggesting that auxin is needed as a trigger for giant cell initiation but not for later enlargement. We suggest that nematodes control auxin distribution in the root and that flavonoids could be responsible for controlling auxin accumulation.

Keywords: auxin transport inhibition, defence response, giant cells, IAA oxidase, in situ fluorescence, lateral roots, nodules, root development.



Full text doi:10.1071/PP98157

© CSIRO 1999

blank image
Subscriber Login
Username:
Password:  

 
PDF (894 KB) $25
 Export Citation
 Print
  
    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014