CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Functional Plant Biology   
Functional Plant Biology
Journal Banner
  Plant Function & Evolutionary Biology
blank image Search
blank image blank image
blank image
  Advanced Search

Journal Home
About the Journal
Editorial Board
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Evolutionary Reviews
Sample Issue
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow PrometheusWiki
blank image
Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 16(6)

Rate Limitation of Non-Steady-State Photosynthesis by Ribulose-1,5-Bisphosphate Carboxylase in Spinach

IE Woodrow and KA Mott

Australian Journal of Plant Physiology 16(6) 487 - 500
Published: 1989


A mathematical framework was developed to analyse rate limitation of non-steady-state photosynthesis following an increase in photon flux density (PFD). This analysis was employed to resolve an exponential phase of the photosynthetic response of Spinacia oleracea L. to a step increase from darkness to moderate PFD. This phase had a relaxation time of approximately 5 min, similar to the relaxation time for the activation of ribulose-1,5-bisphosphate carboxylase (Rubisco) as determined by freeze-clamp experiments following the same change in PFD. Furthermore, as the time in darkness prior to illumination was increased, the exponential phase contributed more to the overall trajectory of photosynthesis following the increase in light. The relaxation time for the increase in the contribution of this phase was 24 min. Freeze-clamp studies showed a relaxation time of 28 min for Rubisco deactivation in the dark. These results, together with measurements of RuP2 levels, suggest that the exponential phase resolved from gas exchange experiments was limited by activation of Rubisco and that Rubisco deactivation in the dark was the reason that this phase contributed more to the overall photosynthetic trajectory as time in darkness increased.

A quantitative expression for the amount of extra photosynthesis that could have been obtained had Rubisco activated instantly following an increase in PFD was derived and discussed in relation to optimal functioning of the system under different environmental conditions.

Full text doi:10.1071/PP9890487

© CSIRO 1989

blank image
Subscriber Login

PDF (656 KB) $25
 Export Citation
Legal & Privacy | Contact Us | Help


© CSIRO 1996-2015