Effects of Eupatorium yellow vein virus infection on photosynthetic rate, chlorophyll content and chloroplast structure in leaves of Eupatorium makinoi during leaf development
Sachiko Funayama-Noguchi A B and Ichiro Terashima AA Department of Biology, Graduate School of Science, Osaka University, Machikaneyama-cho 1-1, Toyonaka, Osaka 560-0043, Japan.
B Corresponding author. Email funayama@bio.sci.osaka-u.ac.jp
Functional Plant Biology 33(2) 165-175 https://doi.org/10.1071/FP05172
Submitted: 11 July 2005 Accepted: 5 October 2005 Published: 3 February 2006
Abstract
Infection of Eupatorium yellow vein geminivirus (EpYVV, formerly called tobacco leaf curl virus, TLCV) causes variegation in Eupatorium makinoi Kawahara et Yahara leaves. We examined changes in photosynthesis during leaf development to clarify what is the primary event when photosynthesis is suppressed in virus-infected E. makinoi leaves. The gas-exchange rate, leaf absorptance, chlorophyll (Chl) and nitrogen contents, leaf anatomy and chloroplast ultrastructure were compared between virus-infected and uninfected E. makinoi leaves at various developmental stages. These photosynthetic properties did not differ between infected and uninfected leaves when they were young. However, when expanded, infected leaves showed lower maximum quantum yield of photosynthetic CO2 uptake in the incident photosynthetically active photon fluence rate (PPFR), which was attributed to their lower Chl contents. The Chla / b ratio was higher and the grana had fewer thylakoids in the infected leaves, which are features common to Chl b-deficient mutants that have defects in Chl synthesis. Our results suggested that, in E. makinoi leaves, EpYVV infection primarily impairs Chl biosynthesis. Possible mechanisms of the suppression of photosynthesis in E. makinoi leaves by virus infection are discussed.
Keywords: geminivirus, photosynthesis, virus infection.
Acknowledgements
We thank Drs S-I Miyazawa, S Yano, and T Saito for their helpful advice on microscopy techniques and anatomical measurements. We also thank Dr K Noguchi, Dr T Hachiya and the members of the Ecophysiological Group, Department of Biology, Graduate School of Science, Osaka University for the kind advice and help. This study was supported by the Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
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