Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Induction of high-affinity NO3 uptake in grapevine roots is an active process correlated to the expression of specific members of the NRT2 and plasma membrane H+-ATPase gene families

Youry Pii A B , Massimiliano Alessandrini A , Katia Guardini A , Anita Zamboni A C and Zeno Varanini A
+ Author Affiliations
- Author Affiliations

A Biotechnology Department, University of Verona, Strada Le Grazie 15, 37134 Verona, Italy.

B Present address: Faculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100 Bolzano, Italy.

C Corresponding author. Email: anita.zamboni@univr.it

Functional Plant Biology 41(4) 353-365 https://doi.org/10.1071/FP13227
Submitted: 31 July 2013  Accepted: 17 October 2013   Published: 26 November 2013

Abstract

The phenomenon of NO3 induction in plant roots has been characterised both in herbaceous and woody plants. Grapevine (Vitis vinifera L.) plants, hydroponically grown, showed an increase in NO3 uptake rate in response to anion treatment for different periods in the nutrient solution after 1 week of NO3 deprivation. The expression profile of the two high-affinity NO3 transporters VvNRT2.4A and VvNRT2.4B, and the gene encoding the accessory protein VvNAR2.2 exhibits a similar trend to that of the anion uptake. The induction, also involving the increase in activity and protein levels of plasma membrane H+-ATPase, is correlated with the expression profile of two (VvHA2 and VvHA4) out of eight putative plasma membrane H+-ATPase genes identified in grapevine genome.

Additional keywords: grapevine physiology, nitrate transporters, nitrogen 15 isotope, proton pump, Vitis vinifera L.


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