Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

A calcineurin B-like protein participates in low oxygen signalling in rice

Viet The Ho A B , Anh Nguyet Tran A , Francesco Cardarelli C , Pierdomenico Perata A and Chiara Pucciariello A D
+ Author Affiliations
- Author Affiliations

A PlantLab, Institute of Life Sciences, Scuola Superiore Sant’Anna, Piazza Martiri della Libertà 33, 56127 Pisa, Italy.

B Present address: Faculty of Biotechnology and Environmental Technology, University of Food Industry, Ho Chi Minh City, Vietnam.

C NEST, Istituto Nanoscienze – CNR and Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy.

D Corresponding author. Email: c.pucciariello@sssup.it

Functional Plant Biology 44(9) 917-928 https://doi.org/10.1071/FP16376
Submitted: 29 October 2016  Accepted: 17 May 2017   Published: 23 June 2017

Abstract

Following the identification of the calcineurin B-like interacting protein kinase 15 (CIPK15), which is a regulator of starch degradation, the low O2 signal elicited during rice germination under submergence has been linked to the sugar sensing cascade and calcium (Ca2+) signalling. CIPK proteins are downstream effectors of calcineurin B-like proteins (CBLs), which act as Ca2+ sensors, whose role under low O2 has yet to be established. In the present study we describe CBL4 as a putative CIPK15 partner, transcriptionally activated under low O2 in rice coleoptiles. The transactivation of the rice embryo CBL4 transcript and CBL4 promoter was influenced by the Ca2+ blocker ruthenium red (RR). The bimolecular fluorescence complementation (BiFC) assay associated to fluorescence recovery after photobleaching (FRAP) analysis confirmed that CBL4 interacts with CIPK15. The CBL4-CIPK15 complex is localised in the cytoplasm and the plasma-membrane. Experiments in protoplasts showed a dampening of α-amylase 3 (RAMY3D) expression after CBL4 silencing by artificial miRNA. Our results suggest that under low O2, the Ca2+ sensor CBL4 interacts with CIPK15 to regulate RAMY3D expression in a Ca2+-dependent manner.

Additional keywords: α-amylase, calcium, CBL4, CIPK15, low oxygen, Oryza sativa.


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