Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Physiological and molecular characterisation for high temperature stress in Lens culinaris

Jitendra Kumar A D , Partha Sarathi Basu B , Sunanda Gupta A , Sonali Dubey A , Debjyoti Sen Gupta A and Narendra Pratap Singh C
+ Author Affiliations
- Author Affiliations

A Division of Crop Improvement, ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur - 208024, India.

B Division of Basic Sciences, ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur - 208024, India.

C Division of Biotechnology, ICAR-Indian Institute of Pulses Research, Kalyanpur, Kanpur - 208024, India.

D Corresponding author. Email: jitendra73@gmail.com

Functional Plant Biology - https://doi.org/10.1071/FP17211
Submitted: 26 July 2017  Accepted: 16 October 2017   Published online: 21 December 2017

Abstract

In the present study, 11 lentil (Lens culinaris Medik) genotypes including heat tolerant and heat sensitive genotypes identified after a screening of 334 accessions of lentil for traits imparting heat tolerance, were characterised based on physiological traits and molecular markers. Results showed a higher reduction in pollen viability among sensitive genotypes (up to 52.3%) compared with tolerant genotypes (up to 32.4%) at 43°C. Higher photosynthetic electron transport rate was observed among heat tolerant genotypes and two heat tolerant lentil genotypes, IG 4258 (0.43) and IG 3330 (0.38) were having highest Fv/Fm values. However, membrane stability was significantly higher in only one heat tolerant genotype, ILL 10712, indicating that different mechanisms are involved to control heat tolerance in lentil. The molecular characterisation of lentil genotypes with 70 polymorphic SSR and genic markers resulted into distinct clusters in accordance with their heat stress tolerance. A functional marker ISM11257 (intron spanning marker) amplifying an allele of 205 bp in size was present only among heat tolerant genotypes, and could be further used in a breeding program to identify heat tolerant lentil genotypes. The findings of this study will contribute to the development of heat tolerant lentil cultivars.

Additional keywords: ETR, functional markers, heat tolerance, lentil, membrane stability, pollen viability.


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