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The effect of simulated microgravity on the Brassica napus seedling proteome
The magnitude and the direction of the gravitational field represent an important environmental factor affecting plant development. In this context, the absence or frequent alterations of the gravity field (i.e. microgravity conditions), might compromise extra-terrestrial agriculture, and, hence, space inhabitation by humans. To overcome the deleterious effects of microgravity, a complete understanding of the underlying changes on the macromolecular level is necessary. However, although microgravity-related changes in gene expression are well-characterized on the transcriptome level, proteomic data are limited. Moreover, the information about the microgravity-induced changes in the seedling proteome during seed germination and the first steps of seedling development is completely missing. One of the valuable tools to assess gravity-related issues is 3-D clinorotation, i.e. rotation in two axes. Therefore, here we address the effects of microgravity, simulated by a two-axial clinostat, on the proteome of 24- and 48-h old seedlings of oilseed rape (Brassica napus). The LC-MS-based proteomic analysis and database search revealed 95 up- and 38 down-regulated proteins in the tryptic digests obtained from the seedlings, subjected to simulated microgravity, with 42 and 52 annotations detected as unique for 24- and 48-h treatment times, respectively. The polypeptides, involved in protein metabolism, transport, and signaling, were annotated as the functional groups most strongly affected by 3-D clinorotation.
FP16378 Accepted 05 October 2017
© CSIRO 2017