Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Increasing Amb a 1 content in common ragweed (Ambrosia artemisiifolia) pollen as a function of rising atmospheric CO2 concentration

Ben D. Singer A C , Lewis H. Ziska B D , David A. Frenz C , Dennis E. Gebhard C and James G. Straka A
+ Author Affiliations
- Author Affiliations

A Department of Biology, Macalester College, 1600 Grand Avenue, St Paul, MN 55105, USA.

B Crop Systems and Global Change Laboratory, United States Department of Agriculture, Agricultural Research Service, 10  300 Baltimore Avenue, Beltsville, MD 20705, USA.

C Multidata LLC, (a subsidiary of Surveillance Data Incorporated), 4838 Park Glen Road, St Louis Park, MN 55416, USA.

D Corresponding author. Email: lziska@asrr.arsusda.gov

Functional Plant Biology 32(7) 667-670 https://doi.org/10.1071/FP05039
Submitted: 21 February 2005  Accepted: 27 April 2005   Published: 7 July 2005

Abstract

Although the impact of increasing atmospheric carbon dioxide concentration ([CO2]) on production of common ragweed (Ambrosia artemisiifolia L.) pollen has been examined in both indoor and outdoor experiments, the relationship between allergen expression and [CO2] is not known. An enzyme-linked immunosorbent assay (ELISA) was used to quantify Amb a 1, ragweed’s major allergen, in protein extracted from pollen of A. artemisiifolia grown at different [CO2] values in a previous experiment. The concentrations used approximated atmospheric pre-industrial conditions (i.e. at the end of the 19th century), current conditions, and the CO2 concentration projected for the middle of the 21st century (280, 370 and 600 μmol mol–1 CO2, respectively). Although total pollen protein remained unchanged, significant increases in Amb a 1 allergen were observed between pre-industrial and projected future [CO2] and between current and projected future [CO2] (1.8 and 1.6 times, respectively). These data suggest that recent and projected increases in [CO2] could directly increase the allergenicity of ragweed pollen and consequently the prevalence and / or severity of seasonal allergic disease. However, genetic and abiotic factors governing allergen expression will need to be better established to fully understand these data and their implications for public health.

Keywords: allergenicity, climate change, public health.


Acknowledgments

We thank Drs Pamela Diggle and Dana Blumenthal for their editorial comments.


References


Ahlholm JU, Helander ML, Savolainen J (1998) Genetic and environment factors affecting the allergenicity of birch (Betula pubescens ssp. Czerepanovii [Orl.] Hamet-Ahti) pollen. Clinical and Experimental Allergy 28, 1384–1388.
CrossRef | PubMed |

Beggs PJ (1998) Pollen and pollen antigen as triggers of asthma — what to measure? Atmospheric Environment 32, 1777–1783.
CrossRef |

Beggs PJ (2004) Impacts of climate change on aeroallergens: past and future. Clinical and Experimental Allergy 34, 1507–1513.
CrossRef | PubMed |

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72, 248–254.
PubMed |


Chapman JA (1986) Aeroallergens of southeastern Missouri. Grana 25, 235–246.

Coca AF (1922) Studies in specific hypersensitiveness. V. The preparation of fluid extracts and solutions for use in the diagnosis and treatment of the allergies with notes on the collection of pollens. Journal of Immunology 7, 163–178.

Engvall E (1980) Enzyme immunoassay ELISA and EMIT. Methods in Enzymology 70, 419–439.
PubMed |


Frenz DA (2001) Interpreting atmospheric pollen counts for use in clinical allergy: allergic symptomology. Annals of Allergy and Clinical Immunology 86, 150–158.

Gergen PJ, Turkeltaub PC, Kovar MC (1987) The prevalence of allergic skin test reactivity to eight common aeroallergens in the US population: results form the second national health and nutrition examination survey. Journal of Allergy and Clinical Immunology 80, 669–679.
CrossRef | PubMed |

Goldfarb AR, Kaplan M (1967) Qualitative and quantitative variations in dwarf ragweed pollens. Journal of Allergy 40, 237–244.
PubMed |


Hjelmroos M, Schumacher MJ, Van Hage-Hamsten M (1995) Heterogeneity of pollen proteins within individual Betula pendula trees. International Archives of Allergy and Immunology 108, 368–376.
PubMed |


Kerim T, Imin N, Weinman JJ, Rolfe BG (2003) Proteomic analysis reveals developmentally expressed rice homologues of grass group II pollen allergens. Functional Plant Biology 30, 843–852.
CrossRef |

Knox RB, Heslop-Harrison J (1971) Pollen-wall proteins: localization of antigenic and allergenic proteins in the pollen-grain walls of Ambrosia spp. (ragweeds). Cytobios 4, 49–54.

Lee YS, Dickinson DB, Schlager D, Velu JG (1979) Antigen E content of pollen from individual plants of short ragweed (Ambrosia artemisiifolia). Journal of Allergy and Clinical Immunology 63, 336–339.
PubMed |


Lewis WH, Imber WE (1975) Allergy epidemiology in the St Louis, Missouri, area: IV. weeds. Annals of Allergy 35, 180–187.
PubMed |


Maasch HJ, Hauck PR, Oliver JD, Geissler W, Wahl R, Winter HG (1987) Allergenic activity of short ragweed pollen (Ambrosia elatior) from different years and / or suppliers: criteria for the selection of an in-house allergen reference preparation. Annals of Allergy 58, 429–434.
PubMed |


Sly RM (1999) Changing prevalence of allergic rhinitis and asthma. Annals of Allergy, Asthma and Immunology 82, 233–252.

Straka JG, Hill HD, Krikava JM, Kools AM, Bloomer JR (1991) Immunochemical studies of ferrochelatase protein: characterization of the normal and mutant protein in bovine and human protoporphyria. American Journal of Human Genetics 48, 72–78.
PubMed |


Tsang VCW, Wilson BC, Peralta JM (1983) Quantitative single-tube kinetic dependent enzyme-linked immunosorbent assay (k-ELISA). Methods in Enzymology 92, 391–403.
PubMed |


Wan S, Yuan T, Bowdish S, Wallace L, Russell SD, Luo Y (2002) Response of an allergenic species, Ambrosia psilostachya (Asteraceae), to experimental warming and clipping: implications for public health. American Journal of Botany 89, 1843–1846.

Wayne P, Foster S, Connolly J, Bazzaz F, Epstein P (2002) Production of allergenic pollen by ragweed (Ambrosia artemisiifolia L.) is increased in CO2-enriched atmospheres. Annals of Allergy, Asthma and Immunology 88, 279–282.

Wodehouse, RP (1971). ‘Hayfever plants (2nd edn).’ (Hafner Publishing: New York)

Ziska LH, Caulfield FA (2000) Rising CO2 and pollen production of common ragweed (Ambrosia artemisiifolia), a known allergy-inducing species: implications for public health. Australian Journal of Plant Physiology 27, 893–898.

Ziska LH, Gebhard DE, Frenz DA, Faulkner SS, Singer BD (2003) Cities as harbingers of climate change: common ragweed, urbanization, and public health. Journal of Allergy and Clinical Immunology 111, 290–295.
CrossRef | PubMed |








Rent Article (via Deepdyve) Export Citation Cited By (73)

View Altmetrics