Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology

How does temperature affect C and N allocation to the seeds during the seed-filling period in pea? Effect on seed nitrogen concentration

Annabelle Larmure A C , Christophe Salon B and Nathalie G. Munier-Jolain B

A ENESAD, Département Agronomie et Environnement, 26 bd Dr Petitjean, BP 87999, 21079 Dijon cedex, France.

B INRA, Unité de Génétique et d’Ecophysiologie des Légumineuses, 17 rue Sully, BP 86510, 21065 Dijon cedex, France.

C Corresponding author. Email:

Functional Plant Biology 32(11) 1009-1017
Submitted: 20 June 2005  Accepted: 4 August 2005   Published: 28 October 2005


The effect of moderate temperature on seed N concentration during the seed-filling period was evaluated in pea (Pisum sativum L.) kept in growth cabinets and the relation between plant assimilate availability and the variation of seed N concentration with temperature was investigated. Seed N concentration of pea was significantly lowered when temperature during the seed-filling period decreased from a day / night temperature of 25 / 20°C to 15 / 10°C. Our results demonstrate that during the seed-filling period mechanisms linked with assimilate availability can modify seed N accumulation rate and / or seed-filling duration between 25 / 20°C and 15 / 10°C. At the lower temperature (15 / 10°C), an increased C availability resulting from an enhanced carbon fixation per degree-day allowed new competing vegetative sinks to grow as pea is an indeterminate plant. Consequently N availability to filling seeds was reduced. Because the rate of seed N accumulation per degree-day mainly depends on N availability to filling seeds, the rate of seed N accumulation was lower at the low temperature of our study (15 / 10°C) than at 25 / 20°C while seed growth rate per degree-day remains unaffected, consequently seed N concentration was reduced. Concomitantly, the increased C availability at the lower temperature prolonged the duration of the seed-filling period.

Keywords: Pisum sativum L., plant C and N assimilate availability, seed filling, seed N concentration, temperature.


Bhullar SS Jenner CF 1985 Differential responses to high temperatures of starch and nitrogen accumulation in the grain of four cultivars of wheat. Australian Journal of Plant Physiology 12 363 375

Blum A Sinmena B Mayer J Golan G Shpiler L 1994 Stem reserve mobilisation supports wheat-grain filling under heat stress. Australian Journal of Plant Physiology 21 771 781

Egli DB 2004 Seed-filling duration and yield of grain crops. Advances in Agronomy 83 243 279

Egli DB Wardlaw IF 1980 Temperature response of seed growth characteristics of soybeans. Agronomy Journal 72 560 564

Gifford RM 1995 Whole plant respiration and photosynthesis of wheat under increased CO2 concentration and temperature: long-term versus short-term distinctions for modelling. Global Change Biology 1 385 396

Gosse G Varlet-Grancher C Bonhomme R Chartier M Allirand JM Lemaire G 1986 Production maximale de matiere seche et rayonnement solaire intercepte par un couvert vegetal. Agronomie 6 47 56

Grandgirard D Munier-Jolain NG Salon C Ney B 2001 Nitrogen nutrition level and temperature effects on vegetative N remobilisation rate and distribution of canopy N during seed filling period in soybean (Glycine max L. Merr.). In ‘Proceedings of the 4th European conference on grain legumes’. Cracow Poland

Guilioni L Wéry J Lecoeur J 2003 High temperature and water deficit may reduce seed number in field pea purely by decreasing plant growth rate. Functional Plant Biology 30 1151 1164 DOI

Harding SC Sheehy JE 1980 Influence of shoot and root temperature on leaf growth, photosynthesis and nitrogen fixation of lucerne. Annals of Botany 45 229 233

Hayati R Egli DB Crafts-Brandner SJ 1995 Carbon and nitrogen supply during seed filling and leaf senescence in soybean. Crop Science 35 1063 1069

Hayati R Egli DB Crafts-Brandner SJ 1996 Independence of nitrogen supply and seed growth in soybean: studies using an in vitro culture system. Journal of Experimental Botany 47 33 40

Hungria M Vargas MAT 2000 Environmental factors affecting N2 fixation in grain legumes in the tropics, with emphasis on Brazil. Field Crops Research 65 151 164 DOI

Hunt LA van der Poorten G Pararajasingham S 1991 Postanthesis temperature effects on duration and rate of grain filling in some winter and spring wheats. Canadian Journal of Plant Science 71 609 617

Jenner CF Uglade TD Aspinall D 1991 The physiology of starch and protein deposition in the endosperm of wheat. Australian Journal of Plant Physiology 18 211 226

Jensen ES 1987 Seasonal patterns of growth and nitrogen fixation in field-grown pea. Plant and Soil 101 29 37

Jeuffroy MH Warembourg FR 1991 Carbon transfer and partitioning between vegetative and reproductive organs in Pisum sativum L. Plant Physiology 97 440 448

Jeuffroy MH Devienne F 1995 A simulation model for assimilate partitioning between pods in pea (Pisum sativum L.) during the period of seed set; validation in field conditions. Field Crops Research 41 79 89 DOI

Karjalainen R Kortet S 1987 Environmental and genetic variation in protein content of peas under northern growing conditions and breeding implications. Journal of Agricultural Science in Finland 59 1 9

Lepo JE Ferrenbach SM 1987 Measurement of nitrogen fixation by direct means. In ‘Symbiotic nitrogen fixation technology’. Elkan GH 221 255 Marcel Dekker New York

Larmure A Munier-Jolain NG 2004 A crop model component simulating N partitioning during seed filling in pea. Field Crops Research 85 135 148 DOI

Lhuillier-Soundélé A Munier-Jolain NG Ney B 1999 a Dependence of seed nitrogen concentration on plant nitrogen availability during the seed filling in pea. European Journal of Agronomy 11 157 166 DOI

Lhuillier-Soundélé A Munier-Jolain NG Ney B 1999 b Influence of nitrogen availability on seed nitrogen accumulation in pea. Crop Science 39 1741 1748

Loveys BR Sheurwater I Pons TL Fitter AH Atkin OK 2002 Growth temperature influences the underlying components of relative growth rate: an investigation using inherently fast- and slow-growing plant species. Plant, Cell & Environment 25 975 987 DOI

Minchin PEH Thorpe MR 1996 What determines carbon partitioning between competing sinks? Journal of Experimental Botany 47 1293 1296

Monteith JL 1972 Solar radiation and productivity in tropical systems. Journal of Applied Ecology 9 747 766

Munier-Jolain NG Ney B 1998 Seed growth rate in legumes. II. Seed growth rate depends on cotyledon cell number. Journal of Experimental Botany 49 1971 1976 DOI

Munier-Jolain NG Munier-Jolain NM Roche R Ney B Duthion C 1998 Seed growth rate in legumes. I. Effect of photoassimilate availability on seed growth rate. Journal of Experimental Botany 49 1963 1969 DOI

Ney B Turc O 1993 Heat-unit-based description of the reproductive development of pea. Crop Science 33 510 514

Ney B Duthion C Fontaine E 1993 Timing of reproductive abortions in relation to cell division, water content, and growth of pea seeds. Crop Science 33 267 270

Pate JS 1985 Physiology of pea — a comparison with other legumes in terms of economy of carbon and nitrogen in whole-plant and organ functioning. In ‘The pea crop’. Hebblewaite PD Heath MC Dawkins TCK 279 296 Butterworths London

Piper EL Boote KJ 1999 Temperature and cultivar effects on soybean seed oil and protein concentration. Journal of the American Oil Chemists’ Society 76 1233 1241

Poggio SL Satorre EH Dethiou S Gonzalo GM 2005 Pod and seed numbers as a function of photothermal quotient during the seed set period of field pea (Pisum sativum) crops. European Journal of Agronomy 22 55 69 DOI

Robertson RN Highkin HR Smydzuk J Went FW 1961 The effect of environmental conditions on the development of pea seeds. Australian Journal of Biological Sciences 14 1 15

SAS Institute 1987 ‘SAS / STAT guide for personal computer. 6th edn.’ SAS Institute Cary, NC

Schiltz S Munier-Jolain NG Jeudy C Burstin J Salon C 2005 Dynamic of exogenous nitrogen partitioning and nitrogen remobilisation from vegetative organs in pea revealed by 15N in vivo labeling throughout seed filling. Plant Physiology 137 1463 1473 DOI

Serraj R Sinclair TR Purcell LC 1999 Symbiotic N2 fixation response to drought. Journal of Experimental Botany 50 143 155 DOI

Shah NH Paulsen GM 2003 Interaction of drought and high temperature on photosynthesis and grain filling in wheat. Plant and Soil 257 219 226 DOI

Singletary GW Banisadr R Keeling PL 1994 Heat stress during grain filling in maize: effects on carbohydrate storage and metabolism. Australian Journal of Plant Physiology 21 829 841

Spiertz JHJ 1977 The influence of temperature and light intensity on grain growth in relation to the carbohydrate and nitrogen economy of the wheat plant. Netherlands Journal of Agricultural Science 25 182 197

Sprent JI Stephens JH Rupela OP 1988 Environmental effects on nitrogen fixation. In ‘World crops: cool season food legumes’. Summerfield RJ 801 810 Kluwer Academic Publishers Dordrecht

Tashiro T Wardlaw IF 1991 The effect of high temperature on the accumulation of dry matter, carbon and nitrogen in the kernel of rice. Australian Journal of Plant Physiology 18 259 265

Thomas LMG Boote KJ Allen LHJ Gallo-Meagher M Davis JM 2003 Elevated temperature and carbon dioxide effects on soybean seed composition and transcript abundance. Crop Science 43 1548 1557

Wardlaw IF Wrigley CW 1994 Heat tolerance in temperate cereals: an overview. Australian Journal of Plant Physiology 21 695 703

Zahedi M Sharma R Jenner FC 2003 Effects of high temperature on grain growth and on metabolites and enzymes in the starch-synthesis pathway in the grains of two wheat cultivars differing in their responses to temperature. Functional Plant Biology 30 291 300 DOI

Zahedi M McDonald G Jenner FC 2004 Nitrogen supply to grain modifies the effects of temperature on starch and protein accumulation during grain filling in wheat. Australian Journal of Agricultural Research 55 551 564 DOI

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