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RESEARCH ARTICLE
Contents Vol 34(2)

Contrasting responses by respiration to elevated CO2 in intact tissue and isolated mitochondria

Dan Bruhn A B C , Joseph T. Wiskich D and Owen K. Atkin A E
+ Author Affiliations
- Author Affiliations

A Department of Biology, The University of York, PO Box 373, York YO10 5YW, UK.

B Risø National Laboratory, Plant Research Department, Building 309, PO Box 49, DK-4000, Denmark.

C Present address: Plant and Soil Science Laboratory, Department of Agricultural Sciences, Faculty of Life Science, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.

D School of Biological Sciences, Flinders University of South Australia, SA 5042, Australia.

E Corresponding author. Email: OKA1@york.ac.uk

Functional Plant Biology 34(2) 112-117 https://doi.org/10.1071/FP06247
Submitted: 3 October 2006  Accepted: 6 December 2006   Published: 12 February 2007

Abstract

The question of whether elevated concentrations of CO2 directly inhibit mitochondrial respiration in plants has received considerable attention. Although there is a growing consensus that elevated [CO2] rarely inhibits respiration of intact tissues, past studies have reported that elevated [CO2] does impact on O2 uptake in isolated mitochondria; what remains unclear, however, is the site(s) where elevated [CO2] impacts on mitochondrial electron transport (ETC). Here we investigated direct effects of [CO2] on respiratory activity of ETC enzymes, intact mitochondria and whole tissues using potato tubers (Solanum tuberosum L. cv. Desiree). Plots of O2 uptake against the redox poise of the ubiquinone (UQ) pool in isolated mitochondria were used to determine whether elevated [CO2] inhibits UQ-reducing and UQ-oxidising pathways differentially. Our results show that mitochondrial respiration was more inhibited via [CO2]/[HCO3–] effects on cytochrome c oxidase (COX) than on succinate dehydrogenase, with [HCO3–] rather than [CO2] inhibiting COX. However, the inhibitory effects at the mitochondrial level did not translate into inhibitory effects at the tissue level. Alternative oxidase (AOX) activity is normally absent in young potato tubers, as was the case in the present study. Thus, the lack of CO2-mediated inhibition at the tissue level was not the result of increases in AOX activity masking the effects of CO2 elsewhere in the respiratory system. We discuss whether the direct impact of elevated [CO2] on respiration is dependent on the rate of metabolic activity and flux control coefficients in individual tissues.


Acknowledgements

DB was supported by the Nordic Academy of Advanced Study, the Danish Research Agency and the Danish Research Training Council. The financial support of a UK Natural Environment Research Council Grant GR8/04360 (to OKA) and an Australian Research Council Grant (to JTW) is acknowledged.


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