Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Ionic relations and osmotic adjustment in durum and bread wheat under saline conditions

Tracey Ann Cuin A , Yu Tian B , Stewart A. Betts A C , Rémi Chalmandrier A D and Sergey Shabala A E

A School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart, Tas. 7001, Australia.

B Institute of Grassland Science, Department of Life Science, Northeast Normal University, ChangChun City, Jilin Province 130024, P.R. China.

C Present address: Department of Agriculture, Fisheries and Forestry, 18 Marcus Clarke Street, Canberra City, ACT 2601, Australia.

D Present address: Institut Polytechnique, LaSalle Beauvais, BP 30313, 60026 Beauvais, France.

E Corresponding author. Email: sergey.shabala@utas.edu.au

Functional Plant Biology 36(12) 1110-1119 http://dx.doi.org/10.1071/FP09051
Submitted: 4 March 2009  Accepted: 10 September 2009   Published: 3 December 2009

Abstract

Wheat breeding for salinity tolerance has traditionally focussed on Na+ exclusion from the shoot, but its association with salinity tolerance remains tenuous. Accordingly, the physiological significance of shoot Na+ exclusion and maintenance of an optimal K+ : Na+ ratio was re-evaluated by studying NaCl-induced responses in 50 genotypes of bread wheat (Triticum aestivum L.) and durum wheat (Triticum turgidum L. ssp. durum) treated with 150 mM NaCl. Overall, Na+ exclusion from the shoot correlated with salinity tolerance in both species and this exclusion was more efficient in bread compared with durum wheat. Interestingly, shoot sap K+ increased significantly in nearly all durum and bread wheat genotypes. Conversely, the total shoot K+ content declined. We argue that this increase in shoot sap K+ is needed to provide efficient osmotic adjustment under saline conditions. Durum wheat was able to completely adjust shoot sap osmolality using K+, Na+ and Cl; it had intrinsically higher levels of these solutes. In bread wheat, organic osmolytes must contribute ~13% of the total shoot osmolality. In contrast to barley (Hordeum vulgare L.), NaCl-induced K+ efflux from seedling roots did not predict salinity tolerance in wheat, implying that shoot, not root K+ retention is important in this species.

Additional keywords: salinity tolerance, screening, shoot sap ion content, Triticum aestivum, Triticum turgidum ssp. durum.


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