Register      Login
Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
Shopping Cart: ( empty )
You are here: Home > Journals > FP > FP09182
RESEARCH ARTICLE
Contents Vol 36(11)

A new screening method for osmotic component of salinity tolerance in cereals using infrared thermography

Xavier R. R. Sirault A B C , Richard A. James A and Robert T. Furbank A B
+ Author Affiliations
- Author Affiliations

A CSIRO Plant Industry, Black Mountain, Corner Clunies Ross Street and Barry Drive, Canberra, ACT 2601, Australia.

B Australian Plant Phenomics Facility – The High Resolution Plant Phenomics Centre, Corner Clunies Ross Street and Barry Drive, Canberra, ACT 2601, Australia.

C Corresponding author. Email: xavier.sirault@csiro.au

This paper originates from a presentation at the 1st International Plant Phenomics Symposium, Canberra, Australia, April 2009.

Functional Plant Biology 36(11) 970-977 https://doi.org/10.1071/FP09182
Submitted: 20 July 2009  Accepted: 15 September 2009   Published: 5 November 2009

Abstract

A high-throughput, automated image analysis protocol for the capture, identification and analysis of thermal images acquired with a long-wave infrared (IR) camera was developed to quantify the osmotic stress response of wheat and barley to salinity. There was a strong curvilinear relationship between direct measurements of stomatal conductance and leaf temperature of barley grown in a range of salt concentrations. This indicated that thermography accurately reflected the physiological status of salt-stressed barley seedlings. Leaf temperature differences between barley grown at 200 mM NaCl and 0 mM NaCl reached 1.6°C – the sensitivity of the IR signal increasing at higher salt concentrations. Seventeen durum wheat genotypes and one barley genotype, known to vary for osmotic stress tolerance, were grown in control (no salt) and 150 mM NaCl treatments to validate the newly-developed automated thermal imaging protocol. The ranking of the 18 genotypes based on both a growth study and the IR measurements was consistent with previous reports in the literature for these genotypes. This study shows the potential of IR thermal imaging for the screening of large numbers of genotypes varying for stomatal traits, specifically those related to salt tolerance.

Additional keywords: Hordeum vulgare, IR, transpiration, Triticum turgidum.


Acknowledgements

The authors wish to thank Dr Rana Munns for helpful comments on the manuscript and the High Resolution Plant Phenomics Centre (Canberra node of the Australian Plant Phenomics Facility) where the research was conducted.


References


Alchanatis V , Cohen Y , Cohen S , Moller M , Meron M , Tsipris J , Orlov V , Naor A , Charit Z (2006) Fusion of IR and multispectral images in the visible range for empirical and model based mapping of crop water status. In ‘2006 ASAE Annual Meeting. Paper #061171’. (American Society of Agricultural and Biological Engineers: St Joseph, MI)

Bernstein L, Hayward HE (1958) Physiology of salt tolerance. Annual Review of Plant Physiology 51, 875–878. open url image1

Epstein E (1980) Responses of plant to saline environments. In ‘Genetic engineering of osmoregulation’. (Eds DW Rains, RC Valentine, A Hollaender) pp. 7–21. (Plenum Press: New York)

Flowers TJ, Troke PF, Yeo AR (1977) The mechanism of salt tolerance in halophytes. Annual Review of Plant Physiology 28, 89–121.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Flowers TJ, Garcia A, Koyama M, Yeo AR (1997) Reading for salt tolerance in crop plants – the role of molecular biology. Acta Physiologiae Plantarum 19, 427–433.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Fricke W, Akhiyarova G, Veselev D, Kudoyarova G (2004) Rapid and tissue specific changes in ABA and in growth rate response to salinity in barley leaves. Journal of Experimental Botany 55, 1115–1123.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Fuchs M (1990) Infrared measurement of canopy temperature and detection of plant water stress. Theoretical and Applied Climatology 42, 253–261.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Horie T, Matsuura S, Takai T, Kuwasaki K, Ohsumi A, Shiraiwa T (2006) Genotypic differences in canopy diffusive conductance measured by a new remote-sensing method and its association with the difference in rice yield potential. Plant, Cell & Environment 29, 653–660.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

James RA, Davenport RJ, Munns R (2006) Physiological characterisation of two genes for Na+ exclusion in durum wheat, Nax1 and Nax2. Plant Physiology 142, 1537–1547.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

James RA, von Caemmerer S, Condon AG, Zwart AB, Munns R (2008) Genetic variation in tolerance to the osmotic stress component of salinity stress in durum wheat. Functional Plant Biology 35, 111–123.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Jones HJ (1999) Use of thermography for quantitative studies of spatial and temporal variation of stomatal conductance over leaf surfaces. Plant, Cell & Environment 22, 1043–1055.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Kaukoranta T, Murto J, Takala J, Tahvonen R (2005) Detection of water deficit in greenhouse cucumber by infrared thermography and reference surfaces. Scientia Horticulturae 106, 447–463.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Kummerlen B , Dauwe S , Schmundt D , Schurr U (1999) Thermography to measure water relations of plant leaves. In ‘Handbook of computer vision and applications’. (Eds B Jahne, H Haubecker, P Geibler) (Academic Press: Boston)

Leinonen I, Jones HJ (2004) Combining thermal and visible imagery for estimating canopy temperature and identifying plant stress. Journal of Experimental Botany 55, 1423–1431.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Liang YK, Dubos C, Dodd IC, Holroyd GH, Hetherington AM, Campbell MM (2005) atMYB61, an R2R3-MYB transcription factor controlling stomatal aperture in Arabidopsis thaliana. Current Biology 15, 1201–1206.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Mather P (1999) ‘Computer processing of remotely-sensed images: an introduction.’ (John Wiley & Sons: New York)

Merlot S, Mustilli AC, Genty B, Notrth H, Lefebvre V, Sotta B, Vavasseur A, Giraudat J (2002) Use of infrared thermal imaging to isolate mutants defective in stomatal regulation. The Plant Journal 30, 601–609.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Munns R, James RA (2003) Screening methods for salinity tolerance: a case study with tetraploid wheat. Plant and Soil 253, 201–218.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology 59, 651–681.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Munns R, James RA, Lauchli A (2006) Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany 57, 1025–1043.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Otsu N (1979) A threshold selection method from gray-level histograms. IEEE Transactions on Systems, Man, and Cybernetics 9, 62–66.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Pearce RS, Fuller MP (2001) Freezing of barley studied by infrared video thermography. Plant Physiology 125, 227–240.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Raskin I, Ladyman JAR (1988) Isolation and characterisation of a barley mutant with abscissic-acid-insensitive stomata. Planta 173, 73–78.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Rebetzke GJ, Condon AG, Richards RA, Read JJ (2001) Phenotypic variation and sampling for leaf conductance in wheat (Triticum aestivum L.) breeding populations. Euphytica 121, 335–341.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Riera M, Valon C, Fenzi F, Giraudat J, Leung J (2005) The genetics of adaptive responses to drought stress: abscisic acid-dependent and abscisic acid-independent signalling components. Physiologia Plantarum 123, 111–119.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Singh KN , Chatrath R (2001) Salinity tolerance. In ‘Application of physiology in wheat breeding’. (Eds MP Reynolds, JJ Ortiz-Monasterio, A McNab) pp. 101–110. (CIMMYT: Mexico)

Tanner CB (1963) Plant temperatures. Agronomy Journal 55, 210–211. open url image1

Weyers JDB, Lawson T (1997) Heterogeneity in stomatal characteristics. Advances in Botanical Research 26, 317–352.
| Crossref | GoogleScholarGoogle Scholar | open url image1