Riboflavin (Vitamin B2) mediated defence induction against bacterial leaf blight: probing through chlorophyll a fluorescence induction O–J–I–P transients
Shasmita A C , Harekrushna Swain A C , Anuprita Ray B , Pradipta K. Mohapatra C , Ramani K. Sarkar B and Arup K. Mukherjee A D
+ Author Affiliations
- Author Affiliations
A Molecular Plant Pathology Laboratory, Division of Crop Protection, Indian Council of Agricultural Research (ICAR) – National Rice Research Institute, Cuttack-753006, Odisha, India.
B Division of Crop Physiology and Biochemistry, Indian Council of Agricultural Research (ICAR) – National Rice Research Institute, Cuttack-753006, Odisha, India.
C Department of Botany, Ravenshaw University, Cuttack-753003, Odisha, India.
D Corresponding authors. Email: arupmukherjee@yahoo.com; titirtua@gmail.com
Functional Plant Biology 45(12) 1251-1261 https://doi.org/10.1071/FP18117
Submitted: 5 May 2018 Accepted: 15 August 2018 Published: 26 September 2018
Abstract
Bacterial leaf blight (BLB) is a serious threat for rice (Oryza sativa L.) cultivation caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae. The pathogen mainly damages the leaf chlorophyllous tissue, resulting in poor photosynthesis and causing up to 50% reductions in grain yield. In the present work, we have compared the structural and functional ability of the chloroplast of three varieties of rice with different degrees of susceptibility (TN1, highly susceptible; IR-20, moderately resistant; DV-85, resistant to BLB) treated with riboflavin (1 and 2 mM) and infected with BLB, with chlorophyll fluorescence as a tool. As indicated by the chlorophyll fluorescence technique, the disease progress curve and yield data, riboflavin acted as an effective vitamin for inducing resistance against BLB. Plants treated with riboflavin showed improved PSII activity, more chlorophyll content and higher yield than the diseased plants.
Additional keywords: chlorophyll fluorescence, PSII, variable fluorescence, Xanthomonas oryzae pv. oryzae.
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