Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology

Effect of Brassinolide and other Growth Regulators on the Germination and Growth of Pollen Tubes of Prunus avium using a Multiple Hanging-drop Assay

FR Hewitt, T Hough, P O'Neill, JM Sasse, EG Williams and KS Rowan

Australian Journal of Plant Physiology 12(2) 201 - 211
Published: 1985


A new multiple hanging-drop assay has been tested with a growth inhibitor, cycloheximide, and a growth-promoter, fusicoccin. This assay has been used to examine the growth-response of pollen-tubes of Prunus avium to brassinolide, a naturally occurring promoter of plant growth. This response is compared with that of indol-3-ylacetic acid, gibberellic acid, and kinetin. Pollen tubes responded to brassinolide and fusicoccin at 1 nM and above, a concentration one order of magnitude lower than that for indol-3-ylacetic acid and gibberellic acid. Kinetin did not stimulate growth of the pollen tubes.

© CSIRO 1985

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