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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 33(7)

Cryptochrome 2 is involved in betacyanin decomposition induced by blue light in Suaeda salsa

Wang Chang-Quan A B, Liu Tao A

A College of Life Science, Shandong University of Technology, Zibo City, Shandong 255049, China.
B Corresponding author. Email: whitewater7006@163.com
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Seeds of the halophyte Suaeda salsa (L.) Pall. were cultured in 24 h dark and 14 h blue light / 10 h dark to examine the role of blue light and the blue-light-absorbing photoreceptor cryptochrome 2 (CRY2) in betacyanin accumulation, hypocotyl elongation and cotyledon opening in S. salsa seedlings. Darkness significantly promoted betacyanin accumulation and hypocotyl elongation but inhibited cotyledon opening. Blue light suppressed betacyanin accumulation and hypocotyl elongation but stimulated cotyledon opening. Betacyanin in S. salsa seedlings decomposed with time in blue light. Western blot analysis showed that CRY2 protein accumulated both in hypocotyls and cotyledons of S. salsa seedlings grown in dark, but degraded with time in blue light, which was paralleled by a decrease of tyrosine hydroxylation activity of tyrosinase, a key enzyme involved in the betalain biosynthesis pathway. These results suggest that CRY2 protein mediates betacyanin decomposition via inactivation of tyrosinase in S. salsa seedlings, and the blue-light-dependent degradation of CRY2 protein is crucial to its function.

Keywords: betacyanin, blue light, cryptochrome 2 (CRY2), dark, Suaeda salsa, western blotting.

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