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Protocols in ecological and environmental plant physiology

 

Article << Previous     |     Next >>   Contents Vol 39(8)

Serpentine tolerance in Mimulus guttatus does not rely on exclusion of magnesium

Emily Palm A C, Kristy Brady A and Elizabeth Van Volkenburgh B

A Department of Biology, University of Washington, Box 351800, Seattle, WA 98195, USA.
B Department of Biology, University of Washington, Box 351330, Seattle, WA 98195, USA.
C Corresponding author. Email: eniniane@uw.edu

Functional Plant Biology 39(8) 679-688 http://dx.doi.org/10.1071/FP12059
Submitted: 23 February 2012  Accepted: 13 June 2012   Published: 9 August 2012


 
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Abstract

The effect of serpentine soil-like low Ca : Mg ratios on growth was investigated in serpentine-adapted and nonadapted populations of Mimulus guttatus Fischer ex DC through soil and hydroponic reciprocal transplants. Adaptation to Ca : Mg ratios in M. guttatus was measured as differences in biomass accumulation, uptake of Ca and Mg, and photosynthetic rates. Serpentine-adapted plants persisted on both serpentine and nonserpentine soils, but nonadapted plants survived only on nonserpentine soil. When grown hydroponically, a low Ca : Mg ratio decreased the biomass of nonadapted plants but serpentine-adapted plants increased in biomass relative to their growth on high Ca : Mg. Internal concentrations of Ca and Mg mirrored those of the growth solution in both populations; however, serpentine-adapted M. guttatus had a higher shoot : root ratio of Mg when grown in low Ca : Mg solutions. Elevated Mg reduced photosynthetic rates in nonadapted plants without changes in chlorophyll concentration or photosystem efficiency. Hydroponic culture isolated the Ca : Mg ratio from other soil characteristics as the dominant factor affecting growth. Differences in the growth of plants from these populations in reciprocal transplant experiments indicate a genetic basis for a tolerance mechanism to low Ca : Mg, but one that is not based on the exclusion of Mg.

Additional keywords: abiotic factors, Ca : Mg ratio, calcium.


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