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Protocols in ecological and environmental plant physiology


Article << Previous     |         Contents Vol 40(12)

Variation in carbon isotope discrimination and its relationship with harvest index in the reference collection of chickpea germplasm

Lakshmanan Krishnamurthy A F, Junichi Kashiwagi B, Satoshi Tobita C, Osamu Ito D, Hari D. Upadhyaya A, Cholenahalli L. L. Gowda A, Pooran M. Gaur A, Madavalam S. Sheshshayee E, Sube Singh A, Vincent Vadez A and Rajeev K. Varshney A

A International Crops Research Institute for the Semiarid Tropics, Patancheru 502 324, Andhra Pradesh, India.
B Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
C Japan International Research Center for Agricultural Sciences, Ohwashi, Tsukuba 305-8686, Japan.
D United Nations University, 1-1-1 Minato Mirai, Nishi-ku, Yokohama 220-8502, Japan.
E University of Agricultural Sciences, GKVK Campus, Bangalore 560 065, India.
F Corresponding author. Email: l.krishnamurthy@cgiar.org
This paper originates from a presentation at theVI International Conference on Legume Genetics and Genomics (ICLGG)Hyderabad, India, 27 October 2012.

Functional Plant Biology 40(12) 1350-1361 http://dx.doi.org/10.1071/FP13088
Submitted: 8 April 2013  Accepted: 24 May 2013   Published: 2 July 2013

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Terminal drought is a major constraint to chickpea productivity. Carbon isotope discrimination (Δ13C), an integrator of plant behaviour influencing transpiration efficiency (TE), is an important component of yield under drought. The variation in Δ13C and its association with yield was assessed in the reference collection of chickpea germplasm. Drought stress reduced shoot biomass by 36–39% and grain yield by 23%. Mean Δ13C was low and the range of genetic variation was high under drought stress. Largely, high Δ13C accessions were early in flowering (40–50 days), moderate in shoot biomass, high in seed yields and high in harvest index (HI). Δ13C was positively correlated with seed yield in both the years under drought stress, only in 2008–09 under optimal irrigation. This positive association was very close with HI. Among the yield components, Δ13C was closely associated with pod numbers per unit area and seed size under drought stress. Path coefficients showed no direct association of Δ13C with grain yield but an indirect negative association through shoot biomass at maturity and a close positive association through HI. The closest association of HI or shoot biomass was seen in the maturity group of accessions that experienced the optimum terminal drought stress.

Additional keywords: genetic variability, heritability, stomatal conductance.


Bartlett MS (1937) Properties of sufficiency and statistical tests. Proceedings of the Royal Society of London. Series A, Mathematical and Physical Sciences 160, 268–282.
CrossRef |

Behboudian MH, Ma Q, Turner NC, Palta JA (2000) Discrimination against 13CO2 in leaves, podwalls, and seeds of water-stressed chickpea. Photosynthetica 38, 155–157.
CrossRef | CAS |

Blum A (2005) Drought resistance, water-use efficiency, and yield potential – are they compatible, dissonant, or mutually exclusive? Australian Journal of Agricultural Research 56, 1159–1168.
CrossRef |

Chen J, Chang SX, Anyia AO (2012) Quantitative trait loci for water-use efficiency in barley (Hordeum vulgare L.) measured by carbon isotope discrimination under rain-fed condition on the Canadian prairies. Theoretical and Applied Genetics 125, 71–90.
CrossRef | PubMed |

Condon AG, Richards RA, Rebetzke GJ, Farquhar GD (2002) Improving intrinsic water-use efficiency and crop yield. Crop Science 42, 122–131.
CrossRef | PubMed |

Condon AG, Richards RA, Rebetzke GJ, Farquhar GD (2004) Breeding for high water-use efficiency. Journal of Experimental Botany 55, 2447–2460.
CrossRef | CAS | PubMed |

Ehdaie B, Hall AE, Farquhar GD, Nguyen HT, Waines JG (1991) Water-use efficiency and carbon isotope discrimination in wheat. Crop Science 31, 1282–1288.
CrossRef |

Farquhar GD, Richards RA (1984) Isotopic composition of plant carbon correlates with water-use efficiency of wheat genotypes. Australian Journal of Plant Physiology 11, 539–552.
CrossRef | CAS |

Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annual Review of Plant Physiology and Plant Molecular Biology 40, 503–537.
CrossRef | CAS |

Febrero A, Fernandez S, Molino-Cano JL, Araus JS (1998) Yield, carbon isotope discrimination, canopy reflectance and cuticular conductance of barley isolines of differing glaucousness. Journal of Experimental Botany 49, 1575–1581.

Ismail AM, Hall AE, Bray EA (1994) Drought and pot size effects on transpiration efficiency and carbon isotope discrimination of cowpea accessions and hybrids. Australian Journal of Plant Physiology 21, 23–35.
CrossRef |

Kashiwagi J, Krishnamurthy L, Upadhyaya HD, Krishna H, Chandra S, Vadez V, Serraj R (2005) Genetic variability of drought- avoidance root traits in the mini-core germplasm collection of chickpea (Cicer arietinum L.). Euphytica 146, 213–222.
CrossRef |

Kashiwagi J, Krishnamurthy L, Singh S, Gaur PM, Upadhyaya HD, Panwar JDS, Basu PS, Ito O, Tobita S (2006) Relationships between transpiration efficiency and carbon isotope discrimination in chickpea (Cicer arietinum L.). International Chickpea and Pigeonpea Newsletter No. 13. pp. 19–21. ICRISAT, Patancheru, Andhra Pradesh, India.

Khan HR, McDonald GK, Rengel Z (2004) Zinc fertilization and water stress affects plant water relations, stomatal conductance and osmotic adjustment in chickpea (Cicer arietinum L.). Plant and Soil 267, 271–284.
CrossRef | CAS |

Khazaie H, Mohammady S, Monneveux P, Stoddard F (2011) The determination of direct and indirect effects of carbon isotope discrimination (Δ), stomatal characteristics and water use efficiency on grain yield in wheat using sequential path analysis. Australian Journal of Crop Science 5, 466–472.

Krishnamurthy L, Kashiwagi J, Gaur PM, Upadhyaya HD, Vadez V (2010) Sources of tolerance to terminal drought in the chickpea (Cicer arietinum L.) minicore germplasm. Field Crops Research 119, 322–330.
CrossRef |

Kumar J, Abbo S (2001) Genetics of flowering time in chickpea and its bearing on productivity in semiarid environments. Advances in Agronomy 72, 107–138.
CrossRef | CAS |

Ludlow MM, Muchow RC (1990) A critical evaluation of traits for improving crop yields in water-limited environments. Advances in Agronomy 43, 107–153.
CrossRef |

Mckay JK, Richards JH, Mitchell-Olds T (2003) Genetics of drought adaptation in Arabidopsis thaliana: I. Pleiotropy contributes to genetic correlations among ecological traits. Molecular Ecology 12, 1137–1151.
CrossRef | CAS | PubMed |

Mohankumar MV, Sheshshayee MS, Rajanna MP, Udayakumar M (2011) Correlation and path analysis of drought tolerance traits on grain yield in rice germplasm accessions. ARPN Journal of Agricultural and Biological Science 6, 70–77.

Passioura JB (1977) Grain yield, harvest index and water use of wheat. Journal of the Australian Institute of Agricultural Science 43, 117–120.

Rebetzke GJ, Condon AG, Richards RA, Farquhar GD (2002) Selection for reduced carbon-isotope discrimination increases aerial biomass and grain yield of rainfed bread wheat. Crop Science 42, 739–745.
CrossRef |

Robinson D, Handley LL, Scrimgeour CM, Gordon DC, Forster BP, Ellis RP (2000) Using stable isotope natural abundances (δ15N and δ13C) to integrate the stress responses of wild barley (Hordeum spontaneum C. Koch.) genotypes. Journal of Experimental Botany 51, 41–50.
CrossRef | CAS | PubMed |

Rosegrant MW, Msangi S, Ringler C, Sulser TB, Zhiu T, Cline SA (2008) ‘International model for policy analysis of agricultural commodities and trade: model description.’ (International Food Policy Research Institute: Washington DC)

Rowland DL, Lamb MC (2005) The effect of irrigation and genotype on carbon and nitrogen isotope composition in peanut (Arachis hypogaea L.) leaf tissue. Peanut Science 32, 48–56.
CrossRef |

Royo C, Martos V, Ramdani A, Villegas D, Rharrabti Y, Garcia del Moral LF (2008) Changes in yield and carbon isotope discrimination of Italian and Spanish durum wheat during the 20th century. Agronomy Journal 100, 352–360.
CrossRef | CAS |

Sheshshayee MS, Bindumadhava H, Shankar AG, Prasad TG, Udayakumar M (2003) Breeding strategies to exploit water use efficiency for crop improvement. Journal of Plant Biology 30, 253–268.

Sinclair TR (2012) Is transpiration efficiency a viable plant trait in breeding for crop improvement? Functional Plant Biology 39, 359–365.
CrossRef |

Tardieu F (2012) Any trait or trait-related allele can confer drought tolerance: just design the right drought scenario. Journal of Experimental Botany 63, 25–31.
CrossRef | CAS | PubMed |

Tobita S, Yamanaka N, Nepomuceno AL, Farias JRB, Neumaier N, Senoo S, Nastasa V (2007) Physiological evaluation of drought tolerance in Brazilian soybean cultivars: water use efficiency and carbon isotope discrimination JIRCAS Working Report 51, 27–33.

Turner NC, Palta JA, Shrestha R, Ludwig C, Siddique KHM, Turner DW (2007) Carbon isotope discrimination is not correlated with transpiration efficiency in three cool-season grain legumes. Journal of Integrative Plant Biology 49, 1478–1483.
CrossRef | CAS |

Upadhyaya HD, Dwivedi SL, Baum M, Varshney RK, Udupa SM, Gowda CLL, Hoisington D, Singh S (2008) Genetic structure, diversity, and allelic richness in composite collection and reference set in chickpea (Cicer arietinum L.). BMC Plant Biology 8, 106
CrossRef | PubMed |

Vadez V, Krishnamurthy L, Hash CT, Upadhyaya HD, Borrell AK (2011) Yield, transpiration efficiency, and water use variations and their relationships in the sorghum reference collection. Crop and Pasture Science 62, 645–655.
CrossRef |

Varshney RK, Nayak SN, May GD, Jackson SA (2009) Next-generation sequencing technologies and their implications for crop genetics and breeding. Trends in Biotechnology 27, 522–530.
CrossRef | CAS | PubMed |

White DS, Wright GC, Bell MJ (1995) Discrimination of carbon isotopes in leaves correlates with variation in water-use efficiency among soybean genotypes grown in the field. ACIAR Food Legumes Newsletter 23, 1–3.

Wright GC, Redden RJ (1995) Variation in water-use efficiency in common bean (Phaseolus vulgaris) genotypes under field conditions. ACIAR Food Legumes Newsletter 23, 4–5.

Wright GC, Rao RCN, Farquhar GD (1994) Water-use efficiency and carbon isotope discrimination in peanut under water deficit conditions. Crop Science 34, 92–97.
CrossRef |

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