CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Functional Plant Biology   
Functional Plant Biology
Journal Banner
  Plant Function & Evolutionary Biology
blank image Search
blank image blank image
blank image
  Advanced Search

Journal Home
About the Journal
Editorial Structure
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Evolutionary Reviews
Sample Issue
Call for Papers
For Authors
General Information
Submit Article
Author Instructions
Open Access
Awards and Prizes
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter LinkedIn

red arrow PrometheusWiki
blank image
Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 41(7)

Distinction and characterisation of salinity tolerant and sensitive rice cultivars as probed by the chlorophyll fluorescence characteristics and growth parameters

Devendra Pratap Singh A B and Ramani Kumar Sarkar A

A Central Rice Research Institute, Cuttack-753 006, India.
B Corresponding author. Email: dpsingh_crri@yahoo.com

Functional Plant Biology 41(7) 727-736 http://dx.doi.org/10.1071/FP13229
Submitted: 1 August 2013  Accepted: 20 January 2014   Published: 14 February 2014

PDF (651 KB) $25
 Supplementary Material
 Export Citation

Soil salinity is a major abiotic stress that limits rice productivity worldwide. The problem is intense – particularly in areas with extremely dry and hot climatic conditions. Designing an effective phenotyping strategy requires thorough understanding of plant survival under stress. The investigation was conducted using 12 rice cultivars differing in salinity tolerance. Among these cultivars, seedling survival on day 10 of salt treatment (12 dS m–1) was above 85% during wet season and 75% during dry season in FL478, AC39416, Pokkali and Kamini. Highly salt-tolerant cultivars maintained greater proportion of green leaf and chlorophyll content under salt stress. Unlike sensitive cultivars, tolerant cultivars taken up less Na+ and more K+, resulting in lower Na+ : K+ ratio in leaf and sheath. Normalised chlorophyll a fluorescence data revealed that the Fv/Fm and PIABS values decreased on days 3 and 7, respectively, of salt stress in susceptible rice cultivar. Salinity factor index (SFI) calculated by giving different weights to relative PIABS values after variable days of salinity stress clearly distinguished the level of tolerance among rice cultivars. The SFI can be used for grouping of moderately to highly salt-tolerant cultivars based on their tolerance level. We conclude that maintenance of greater proportion of green leaf, and restricted transport of Na+ to sheath and leaf helps the plant to counteract adverse effects of salinity on rice growth.

Additional keywords: chlorophyll fluorescence, green-leaf proportion, Na+ : K+ ratio, rice cultivars, salinity factor index.


Amirjani MR (2011) Effects of salinity stress on growth, sugar content, pigments and enzyme activity of rice. International Journal of Botany 7, 73–81.
CrossRef | CAS |

Falqueto AR, dos Santos PN, Fontes RV, Silva DM (2012) Analysis of chlorophyll a fluorescence of two mangrove species of Vitória Bay (ES, Brazil) to natural variation of tide. Revista Biociências 18, 14–23.

Glaszmann JC, Kilian B, Upadhyaya HD, Varshney RK (2010) Accessing genetic diversity for crop improvement. Current Opinion in Plant Biology 13, 167–173.
CrossRef | CAS | PubMed |

Huther CM, Ramm A, Rombaldi CV, Bacarin MA (2013) Physiological response to heat stress of tomato ‘Micro-tom’ plants expressing high and low levels of mitochondrial sHSP23.6 protein. Plant Growth Regulation 70, 175–185.
CrossRef | CAS |

Lafitte HR, Ismail A, Bennett J (2004) Abiotic stress tolerance in rice for Asia: progress and the future. ‘New directions for a diverse planet. Proceedings of the 4th international crop science congress’. Available at www.cropscience.org.au [Accessed 10 November 2013]

Lazár D (2006) The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light. Functional Plant Biology 33, 9–30.
CrossRef |

Mitsuya S, Kawasaki M, Taniguchi M, Miyake H (2003) Relationship between salinity-induced damages and aging in rice leaf tissues. Plant Production Science 6, 213–218.
CrossRef |

Moradi F, Ismail AM (2007) Responses of photosynthesis, chlorophyll fluorescence and ROS scavenging system to salt stress during seedling and reproductive stages in rice. Annals of Botany 99, 1161–1173.
CrossRef | CAS | PubMed |

Munns R (1993) Physiological processes limiting plant growth in saline soil: some dogmas and hypotheses. Plant, Cell & Environment 16, 15–24.
CrossRef | CAS |

Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology 59, 651–681.
CrossRef | CAS | PubMed |

Nakhoda B, Leung H, Mendioro MS, Mohammadi-nejad G, Ismail AM (2012) Isolation, characterization, and field evaluation of rice (Oryza sativa L., Var. IR64) mutants with altered responses to salt stress. Field Crops Research 127, 191–202.
CrossRef |

Oukarroum A, Madidi SE, Schansker G, Strasser RJ (2007) Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. Environmental and Experimental Botany 60, 438–446.
CrossRef | CAS |

Panda D, Rao DN, Sharma SG, Strasser RJ, Sarkar RK (2006) Submergence effect on rice genotypes during seedling stage: probing of submergence driven changes of photosystem 2 by chlorophyll a fluorescence induction O-J-I-P transients. Photosynthetica 44, 69–75.
CrossRef | CAS |

Sarkar RK, Bhattacharjee B (2011) Rice genotypes with Sub1 QTL differ in submergence tolerance, elongation ability during submergence, and re-generation growth at re-emergence. Rice
CrossRef |

Sarkar RK, Panda D (2009) Distinction and characterisation of submergence tolerant and sensitive rice cultivars, probed by the fluorescence OJIP rise kinetics. Functional Plant Biology 36, 222–233.
CrossRef | CAS |

Sarkar RK, Panda D, Reddy JN, Patnaik SSC, Mackill DJ, Ismail AM (2009) Performance of submergence tolerant rice (Oryza sativa) genotypes carrying the Sub1 quantitative trait locus under stressed and non-stressed natural field conditions. Indian Journal of Agricultural Sciences 79, 876–883.

Sarkar RK, Mahata KR, Singh DP (2013) Differential responses of antioxidant system and photosynthetic characteristics in four rice cultivars differing in sensitivity to sodium chloride stress. Acta Physiologiae Plantarum 35, 2915–2926.
CrossRef | CAS |

Singh DP, Mahata KR, Saha S, Ismail AM (2010) Crop diversification for improving water productivity and rural livelihoods in coastal saline soils of the Mahanadi delta, India. In ‘Tropical deltas and coastal zones: food production, communities and environment at the land-water interface. Vol. 9’. (Eds CT Hoanh, BW Szuster, K Suan-peng, AM Ismail, AD Noble) pp. 249–263. (CABI: Oxfordshire, UK)

Strasser RJ, Tsimilli-Michael M (2001) Stress in plants, from daily rhythm to global changes, detected and quantified by the JIP test. Chimie Nouvelle [SRC] 75, 3321–3326.

Strasser RJ, Srivastava A, Tsimilli-Michael M (2000) The fluorescence transient as a tool to characterize and screen photosynthetic samples. In ‘Probing photosynthesis: mechanism, regulation and adaptation’. (Eds M Yunus, U Pathre, P Mohantys) pp. 443–480. (Taylor and Francis: London, UK)

Strauss AJ, Krüger GHJ, Strasser RJ, Van Heerden PDR (2006) Ranking of dark chilling tolerance in soybean genotypes probed by the chlorophyll a fluorescence transient O–J–I–P. Environmental and Experimental Botany 56, 147–157.
CrossRef | CAS |

Thomson MJ, de Ocampo M, Egdane J, Rahman MA, Sajise AG, Adorada DL, Tumimbang-Raiz E, Blumwald E, Seraj ZI, Singh RK, Gregorio GB, Ismail AM (2010) Characterizing the saltol quantitative trait locus for salinity tolerance in rice. Rice 3, 148–160.
CrossRef |

Ul Haq T, Gorham J, Akhtar J, Akhtar N, Steele KA (2010) Dynamic quantitative trait loci for salt stress components on chromosome 1 of rice. Functional Plant Biology 37, 634–645.
CrossRef |

Ul Haq T, Akhtar J, Steele KA, Munns R, Gorham J (2013) Reliability of ion accumulation and growth components for selecting salt tolerant lines in large populations of rice. Functional Plant Biology
CrossRef |

Wankhade SD, Cornejo MJ, Mateu-Andrés I, Sanz A (2013) Morpho-physiological variations in response to NaCl stress during vegetative and reproductive development of rice. Acta Physiologiae Plantarum 35, 323–333.
CrossRef | CAS |

Xing D, Wu Y (2012) Photosynthetic response of three climber plant species to osmotic stress induced by polyethylene glycol (PEG) 6000. Acta Physiologiae Plantarum 34, 1659–1668.
CrossRef | CAS |

Yoshida S, Forno D, Cock J, Gomez KA (1976) ‘Laboratory manual for physiological studies of rice.’ (International Rice Research Institute: Los Banós, Philippines)

Zeng L, Lesch SM, Catherine M, Grieve CM (2003) Rice growth and yield respond to changes in water depth and salinity stress. Agricultural Water Management 59, 67–75.
CrossRef |

Subscriber Login

Legal & Privacy | Contact Us | Help


© CSIRO 1996-2015