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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 42(2)

Higher flower and seed number leads to higher yield under water stress conditions imposed during reproduction in chickpea

Raju Pushpavalli A B, Mainassara Zaman-Allah C, Neil C. Turner D, Rekha Baddam A, Mandali V. Rao B and Vincent Vadez A E

A International Crops Research Institute for the Semiarid Tropics, Crop Physiology Laboratory, Patancheru 502 324, Telangana, India.
B Department of Plant Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
C CIMMYT, PO Box MP 163, Mount Pleasant Harare, Zimbabwe.
D The UWA Institute of Agriculture and Centre for Plant Genetics and Breeding, M080, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
E Corresponding author. Email: v.vadez@cgiar.org

Functional Plant Biology 42(2) 162-174 http://dx.doi.org/10.1071/FP14135
Submitted: 6 May 2014  Accepted: 28 July 2014   Published: 4 September 2014

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The reproductive phase of chickpea (Cicer arietinum L.) is more sensitive to water deficits than the vegetative phase. The characteristics that confer drought tolerance to genotypes at the reproductive stage are not well understood; especially which characteristics are responsible for differences in seed yield under water stress. In two consecutive years, 10 genotypes with contrasting yields under terminal drought stress in the field were exposed to a gradual, but similar, water stress in the glasshouse. Flower number, flower + pod + seed abortion percentage, pod number, pod weight, seed number, seed yield, 100-seed weight (seed size), stem + leaf weight and harvest index (HI) were recorded in well watered plants (WW) and in water-stressed plants (WS) when the level of deficit was mild (phase I), and when the stress was severe (phase II). The WS treatment reduced seed yield, seed and pod number, but not flower + pod + seed abortion percentage or 100-seed weight. Although there were significant differences in total seed yield among the genotypes, the ranking of the seed yield in the glasshouse differed from the ranking in the field, indicating large genotype × environment interaction. Genetic variation for seed yield and seed yield components was observed in the WW treatment, which also showed differences across years, as well as in the WS treatment in both the years, so that the relative seed yield and relative yield components (ratio of values under WS to those under WW) were used as measures of drought tolerance. Relative total seed yield was positively associated with relative total flower number (R2 = 0.23 in year 2) and relative total seed number (R2 = 0.83, R2 = 0.79 in years 1 and 2 respectively). In phase I (mild stress), relative yield of seed produced in that phase was found to be associated with the flower number in both the years (R2 = 0.69, R2 = 0.76 respectively). Therefore, the controlled drought imposition that was used, where daily water loss from the soil was made equal for all plants, revealed genotypic differences in the sensitivity of the reproductive process to drought. Under these conditions, the seed yield differences in chickpea were largely related to the capacity to produce a large number of flowers and to set seeds, especially in the early phase of drought stress when the degree of water deficit was mild.

Additional keywords: flower number, fraction of transpirable soil water, normalised transpiration ratio, pod number, seed number.


Berger JD, Turner NC, Siddique KHM, Knights EJ, Brinsmead RB, Mock I, Edmondson C, Khan TN (2004) Genotype by environment studies across Australia reveal the importance of phenology for chickpea (Cicer arietinum L.) improvement. Australian Journal of Agricultural Research 55, 1071–1084.
CrossRef |

Clarke HJ, Siddique KHM (1998) Growth and development. In ‘The Chickpea book: a technical guide to chickpea production. Bulletin 1326’. (Eds SP Loss, N Brandon, KHM Siddique) pp. 3–10. (Department of Agriculture of Western Australia: South Perth)

Croser JS, Clarke HJ, Siddique KHM, Khan TN (2003) Low temperature stress: implications for chickpea (Cicer arietinum L.) improvement. Critical Reviews in Plant Sciences 22, 185–219.
CrossRef |

Davies SL, Turner NC, Siddique KHM, Plummer JA, Leport L (1999) Seed growth of desi and kabuli chickpea (Cicer arietinum L.) in a short-season Mediterranean-type environment. Australian Journal of Experimental Agriculture 39, 181–188.
CrossRef |

Davies SL, Turner NC, Palta JA, Siddique KHM, Plummer JA (2000) Remobilization of carbon and nitrogen supports seed filling in chickpea subjected to water deficit. Australian Journal of Agricultural Research 51, 855–866.
CrossRef |

Fang X, Turner NC, Yan G, Li F, Siddique KHM (2010) Flower numbers, pod production, pollen viability, and pistil function are reduced and flower and pod abortion increased in chickpea (Cicer arietinum L.) under terminal drought. Journal of Experimental Botany 61, 335–345.
CrossRef | CAS | PubMed |

Fang XW, Turner NC, Li FM, Siddique KHM (2011) An early transient water deficit reduces flower number and pod production but increases seed size in chickpea (Cicer arietinum L.) Crop and Pasture Science 62, 481–487.
CrossRef | CAS |

FAOSTAT (2013) FAOSTAT database. Available at http://faostat3.fao.org/faostat-gateway/go/to/download/Q/QC/E [Verified 12 August 2014]

Gowda CLL, Parthasarathy Rao P, Tripathy S, Gaur PM, Deshmukh RB (2009) Regional shift in chickpea production in India. In ‘Milestones in food legumes research’. (Eds A Masood, S Kumar) pp. 21–35. (Indian Institute of Pulses Research: Kanpur, India)

Harb A, Krishnan A, Ambavaram MMR, Pereira A (2010) Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth. Plant Physiology 154, 1254–1271.
CrossRef | CAS | PubMed |

Krishnamurthy L, Kashiwagi J, Gaur PM, Upadhyaya HD, Vadez V (2010) Sources of tolerance to terminal drought in the chickpea (Cicer arietinum L.) minicore germplasm. Field Crops Research 119, 322–330.
CrossRef |

Leport L, Turner NC, French RJ, Tennant D, Thomson BD, Siddique KHM (1998) Water relations, gas exchange and growth of cool-season grain legumes in a Mediterranean-type environment. European Journal of Agronomy 9, 295–303.
CrossRef |

Leport L, Turner NC, French RJ, Barr MD, Duda R, Davies SL, Tennant D, Siddique KHM (1999) Physiological responses of chickpea genotypes to terminal drought in a Mediterranean-type environment. European Journal of Agronomy 11, 279–291.
CrossRef |

Leport L, Turner NC, Davies SL, Siddique KHM (2006) Variation in pod production and abortion among chickpea cultivars under terminal drought. European Journal of Agronomy 24, 236–246.
CrossRef |

Ray JD, Sinclair TR (1998) The effect of pot size on growth and transpiration of maize and soybean during water deficit stress. Journal of Experimental Botany 49, 1381–1386.
CrossRef | CAS |

Siddique KHM, Brinsmead RB, Knight R, Knights E, Paull JG, Rose IA (2000) Adaptation of chickpea (Cicer arietinum L.) and faba bean (Viciafaba L.) to Australia. In ‘Linking research and marketing opportunities for pulses in the 21st century’. (Ed. R Knight) pp. 289–303. (Kluwer Academic Publishers: Dordrecht, The Netherlands)

Sinclair TR, Ludlow MM (1985) Who taught plants thermodynamics? The unfulfilled potential of plant water potential. Australian Journal of Plant Physiology 12, 213–217.
CrossRef |

Turner NC (2003) Adaptation to drought: lessons from studies with chickpea. Indian Journal of Plant Physiology 11–17.

Turner NC, Davies SL, Plummer JA, Siddique KHM (2005) Seed filling in grain legumes under water deficits, with emphasis on chickpeas. Advances in Agronomy 87, 211–250.
CrossRef | CAS |

Upadhyaya HD, Ortiz R (2001) A mini-core subset for capturing diversity and promoting utilization of chickpea genetic resources in crop improvement. Theoretical and Applied Genetics 102, 1292–1298.
CrossRef |

Upadhyaya HD, Dwivedi SL, Baum M, Varshney RK, Udupa SM, Gowda CLL, Hoisington D, Singh S (2008) Genetic structure, diversity, and allelic richness in composite collection and reference set in chickpea (Cicer arietinum L.). BMC Plant Biology 8, 106
CrossRef | PubMed |

Vadez V, Sinclair TR (2001) Leaf ureide degradation and N2 fixation tolerance to water deficit in soybean. Journal of Experimental Botany 52, 153–159.
CrossRef | CAS | PubMed |

Vadez V, Rashmi M, Sindhu K, Muralidharan M, Pushpavalli R, Turner NC, Krishnamurthy L, Gaur PM, Colmer TD (2012) Large number of flowers and tertiary branches, and higher reproductive success increase yields under salt stress in chickpea. European Journal of Agronomy 41, 42–51.
CrossRef |

Zaman-Allah M, Jenkinson DM, Vadez V (2011a) A conservative pattern of water use, rather than deep or profuse rooting, is critical for the terminal drought tolerance of chickpea. Journal of Experimental Botany 62, 4239–4252.
CrossRef | CAS | PubMed |

Zaman-Allah M, Jenkinson DM, Vadez V (2011b) Chickpea genotypes contrasting for seed yield under terminal drought stress in the field differ for traits related to the control of water use. Functional Plant Biology 38, 270–281.
CrossRef |

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