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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 41(12)

Post–flood nitrogen and basal phosphorus management affects survival, metabolic changes and anti-oxidant enzyme activities of submerged rice (Oryza sativa)

Priyanka Gautam A C D, Banwari Lal A C, Rajagounder Raja A, Mirza Jaynul Baig B, Deepika Haldar A, Liza Rath A, Mohammad Shahid A, Rahul Tripathi A, Sangita Mohanty A, Pratap Bhattacharyya A and Amaresh Kumar Nayak A

A Division of Crop Production, Central Rice Research Institute, Cuttack, Odisha 753 006, India.
B Division of Biochemistry, Physiology and Environmental Sciences, Central Rice Research Institute, Cuttack, Odisha 753 006, India.
C These authors contributed equally to this work.
D Corresponding author. Email: priyanakagautam@gmail.com

Functional Plant Biology 41(12) 1284-1294 http://dx.doi.org/10.1071/FP14093
Submitted: 24 March 2014  Accepted: 3 June 2014   Published: 5 August 2014

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Flooding is one of the major harmful abiotic stresses in the low lying areas of Asia and crop losses due to submergence are considerably high. Along with plant breeding techniques, agronomic management options in general and nutrient management in particular should be taken into consideration. Response of Sub 1 and non-Sub1 cultivars of rice to post-flood nitrogen (N) management under variable flood water was compared at maximum tillering stage. Submergence tolerance on survival, leaf senescence, metabolic changes, and anti-oxidant enzymatic activities were evaluated. Sub1 cultivars proved their superiority over IR-20 in terms of significantly higher survival, anti-oxidant enzymes and lower metabolic changes. Turbid water resulted in lower survival because of poor light transmission, chlorophyll retention and silt deposition. Basal phosphorus reduced the elongation, senescence and ethylene accumulation. Post-flood foliar spray of urea substantially increased the chlorophyll, soluble sugars and extenuated ethylene accumulation resulting in significantly higher survival. These nutrient management options can provide opportunities for better survival and productivity even under turbid water, helping farmers to cope with the existing problems in flood-prone areas.

Additional keywords: anti-oxidant enzymes, chlorophyll, elongation, turbidity.


Ahmed S, Nawata E, Hosokawa Y, Domae Y, Sakuratani T (2002) Alteration in photosynthesis and some antioxidant enzymatic activities of mung bean subjected to waterlogging. Plant Science 163, 117–123.
CrossRef | CAS |

Alamgir H, Uddin SN (2011) Mechanisms of waterlogging tolerance in wheat: morphological and metabolic adaptations under hypoxia or anoxia. Australian Journal of Crop Science 5, 1094–1110.

Bailey-Serres J, Voesenek LACJ (2008) Flooding stress: acclimations and genetic diversity. Annual Review of Plant Biology 59, 313–339.
CrossRef | CAS | PubMed |

Bailey-Serres J, Fukao T, Ronald P, Ismail AM, Heuer S, Mackill D (2010) Submergence tolerant rice: SUB1’s journey from landrace to modern cultivar. Rice 3, 138–147.
CrossRef |

Cakmak I, Marschner H (1992) Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiology 98, 1222–1227.
CrossRef | CAS | PubMed |

Chance B, Maehly AC (1955) Assay of catalases and peroxidases. In ‘Methods in enzymology. Vol. 2’. (Eds SP Colowick, NO Kaplan) pp. 764–775. (Academic Press: New York)

Choudhury SR, Choudhury MA (1985) Hydrogen peroxide metabolism as an index of water stress tolerance in jute. Physiologia Plantarum 65, 503–507.

Crawford RMM (2003) Seasonal differences in plant responses to flooding and anoxia. Canadian Journal of Botany 81, 1224–1246.
CrossRef | CAS |

Das KK, Panda D, Sarkar RK, Reddy JN, Ismail AM (2009) Submergence tolerance in relation to variable floodwater conditions in rice. Environmental and Experimental Botany 66, 425–434.
CrossRef | CAS |

Ella ES, Ismail AM (2006) Seedling nutrient status before submergence affects survival after submergence in rice. Crop Science 46, 1673–1681.
CrossRef | CAS |

Ella E, Kawano N, Yamauchi Y, Tanaka K, Ismail AM (2003) Blocking ethylene perception enhances flooding tolerance in rice seedlings. Functional Plant Biology 30, 813–819.
CrossRef | CAS |

Fukao T, Bailey-Serres J (2008) Submergence tolerance conferred by SUB 1A is mediated by SLR 1 and SLRL1 restriction of gibberellin responses in rice. Proceedings of the National Academy of Sciences of the United States of America 105, 16814–16819.
CrossRef | CAS | PubMed |

Fukao T, Yeung E, Bailey-Serres J (2012) The submergence tolerance gene SUB1A delays leaf senescence under prolonged darkness through hormonal regulation in rice. Plant Physiology 160, 1795–1807.
CrossRef | CAS | PubMed |

Gautam P, Nayak AK, Lal B, Bhattacharyya P, Tripathi R, Shahid M, Mohanty S, Raja R, Panda BB (2014) Submergence tolerance in relation to application time of nitrogen and phosphorus in rice (Oryza sativa L.). Environmental and Experimental Botany 99, 159–166.
CrossRef | CAS |

Giannopolitis CN, Ries SK (1977) Superoxide dismutases. I: Occurrence in higher plants. Plant Physiology 115, 159–169.

Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I: Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125, 189–198.
CrossRef | CAS | PubMed |

Jackson MB (2008) Ethylene-promoted elongation: an adaptation to submergence stress. Annals of Botany 101, 229–248.
CrossRef | CAS | PubMed |

Jackson MB, Ram PC (2003) Physiological and molecular basis of susceptibility and tolerance of rice plants to complete submergence. Annals of Botany 91, 227–241.
CrossRef | CAS | PubMed |

Jackson MB, Waters I, Setter TL, Greenway H (1987) Injury to rice plants caused by complete submergence: a contribution by ethylene (ethene). Journal of Experimental Botany 38, 1826–1838.
CrossRef | CAS |

Kawano N, Ella E, Ito O, Yamauchi Y, Tanaka K (2002) Metabolic changes in rice seedlings with different submergence tolerance after desubmergence. Environmental and Experimental Botany 47, 195–203.
CrossRef | CAS |

Kende H, Hanson AD (1976) Relationship between ethylene evolution and senescence in morning-glory flower tissue. Plant Physiology 57, 523–527.
CrossRef | CAS | PubMed |

Larson RA (1988) The antioxidants of higher plants. Phytochemistry 27, 969–978.
CrossRef | CAS |

Ling J, Ming-Yu H, Chun-Ming W, Jian-Min W (2004) Quantitative trait loci and epistatic analysis of seed anoxia germinability in rice (Oryza sativa). Rice Science 11, 238–244.

Mackill DJ, Ismail AM, Singh US, Labios RV, Paris TR (2012) Development and rapid adoption of submergence-tolerant (Sub1) rice varieties. Advances in Agronomy 115, 299–352.
CrossRef |

Panda D, Sarkar RK (2012) Leaf photosynthetic activity and antioxidant defense associated with sub1 QTL in rice subjected to submergence and subsequent re-aeration. Rice Science 19, 108–116.
CrossRef |

Panda D, Sarkar RK (2013) Characterization of leaf gas exchange and anti-oxidant defense of rice (Oryza sativa l.) cultivars differing in submergence tolerance owing to complete submergence and consequent re-aeration. Agricultural Research 2, 301–308.
CrossRef | CAS |

Panda D, Rao DN, Sharma SG, Strasser RJ, Sarkar RK (2006) Submergence effect on rice genotypes during seedling stage: probing of submergence driven changes of photosystem 2 by chlorophyll a fluorescence induction O-J-I-P transients. Photosynthetica 44, 69–75.
CrossRef | CAS |

Porra RJ (2002) The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research 73, 149–156.
CrossRef | CAS | PubMed |

Ram PC, Singh AK, Singh BB, Singh VK, Singh HP, Setter TL, Singh VP, Singh RK (1999) Environmental characterization of floodwater in eastern India: relevance to submergence tolerance of lowland rice. Experimental Agriculture 35, 141–152.
CrossRef |

Ram PC, Singh BB, Singh AK, Ram P, Singh PN, Singh HP, Boamfa I, Harren F, Santosa E, Jackson MB, Setter TL, Reuss J, Wade LJ, Singh VP, Singh RK (2002) Submergence tolerance in rainfed lowland rice: physiological basis and prospects for cultivar improvement through marker-aided breeding. Field Crops Research 76, 131–152.
CrossRef |

Rama Krishnayya G, Setter TL, Sarkar RK, Krishnan P, Ravi I (1999) Influence of P application to floodwater on oxygen concentrations and survival of rice during complete submergence. Experimental Agriculture 35, 167–180.
CrossRef |

Sarkar RK (1997) Saccharide content and growth parameters in relation with flooding tolerance in rice. Biologia Plantarum 40, 597–603.
CrossRef | CAS |

Sarkar RK, Das S, Ravi I (2001) Changes in certain antioxidative enzymes and growth parameters as a result of complete submergence and subsequent re-aeration of rice cultivars differing in submergence tolerance. Journal Agronomy & Crop Science 187, 69–74.
CrossRef | CAS |

Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiology 101, 7–12.

Setter TL, Kupkanchanakul K, Bhekasut B, Kupkanchanapul T, Weingweera A, Greenway A (1987) Concentrations of CO2 and O2 in floodwater and in internodal lacunae of floating rice growing at 1–2 metre water depths. Plant, Cell & Environment 10, 767–776.

Sone C, Ito O, Sakagami JI (2012) Characterizing submergence survival strategy in rice via chlorophyll fluorescence. Journal Agronomy & Crop Science 198, 152–160.
CrossRef | CAS |

Waters IW, Armstrong TCJ, Setter TL (1989) Diurnal changes in radial oxygen loss and ethanol metabolism in roots of submerged and non-submerged rice seedlings. New Phytologist 113, 439–451.
CrossRef | CAS |

Xu K, Xu X, Fukao T, Canlas P, Maghirang-Rodriguez R, Heuer S, Ismail AM, Bailey-Serres J, Ronald PC, Mackill DJ (2006) Sub1A is an ethylene responsive-factor like gene that confers submergence tolerance to rice. Nature 442, 705–708.
CrossRef | CAS | PubMed |

Yamada N (1959) Physiological basis of resistance of rice plant against overhead flooding. Bulletin of the National Institute of Agricultural Sciences, Series D (Plant physiology, Genetics and Crops in General) 8, 1–112.

Yoshida S, Forno D, Cock J, Gomez KA (1976) ‘Laboratory manual for physiological studies of rice.’ (International Rice Research Institute: Los Banos, Philippines)

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