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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 41(9)

Root traits and cellular level tolerance hold the key in maintaining higher spikelet fertility of rice under water limited conditions

Bheemanahalli R. Raju A, Beerasandra R. Narayanaswamy A, Malagondanahalli V. Mohankumar A, Kambalimath K. Sumanth A, Mavinahalli P. Rajanna B, Basavaiah Mohanraju A, Makarla Udayakumar A and Madavalam S. Sheshshayee A C

A Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru-560 065, India.
B Zonal Agricultural Research Station, VC Farm, Mandya-571 405, India.
C Corresponding author. Email: msheshshayee@hotmail.com

Functional Plant Biology 41(9) 930-939 http://dx.doi.org/10.1071/FP13291
Submitted: 10 October 2013  Accepted: 8 March 2014   Published: 7 May 2014

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Reduced spikelet fertility appears to be one of the major factors responsible for the decreased rice grain yield when cultivated under semi irrigated aerobic condition. We demonstrate that genotypes with better root systems coupled with higher cellular level tolerance (CLT) can significantly improve spikelet fertility under semi-irrigated aerobic condition in the field. A set of 20 contrasting rice accessions differing in root traits and CLT with significant molecular diversity were subjected to specific soil moisture regimes during a period between five days before and 10 days after anthesis. Lowest spikelet fertility was observed among the plants grown under water limited (WL) conditions followed by the plants grown aerobically in field conditions (AF). Deep rooted genotypes generally maintained higher spikelet fertility under both WL and AF conditions. Furthermore, genotypes that had high roots biomass as well as high CLT recorded the lowest reduction in spikelet fertility under WL and AF compared with the low root and low CLT genotype. This study emphasised the relevance of combining water acquisition and CLT for improving field level tolerance of rice to water limitation. Such genotypes recorded significantly higher grain yield under stress as well as well watered conditions. The study led to the identification of promising trait donor genotypes which can be exploited in breeding to develop superior trait pyramided cultivars suitable for semi irrigated aerobic cultivation.

Additional keywords: CLT, rice, root traits, semi irrigated aerobic cultivation, spikelet fertility.


Abdullah Z, Khan MA, Flowers TJ (2001) Causes of sterility in seed set of rice under salinity stress. Journal Agronomy & Crop Science 187, 25–32.
CrossRef |

Araus JL, Slafer GA, Reynolds MP, Royo C (2002) Plant breeding and drought in C3 cereals: what should we breed for? Annals of Botany 89, 925–940.
CrossRef | PubMed |

Araus JL, Slafer GA, Royo C, Serret M (2008) Breeding for yield potential and stress adaptation in cereals. Critical Reviews in Plant Sciences 27, 377–412.
CrossRef |

Barker R, Dawe D, Tuong TP, Bhuiyan SI, Guerra LC (1998) The outlook for water resources in the year 2020: challenges for research on water management in rice production. In ‘Assessment and orientation towards the 21st century. Proceedings of 19th session of the International Rice Commission’. pp. 96–109. (FAO: Rome)

Blum A (2002) Drought tolerance – is it a complex trait. In ‘Field screening for drought tolerance in crop plants with emphasis of rice’. (Eds NP Saxena, JC O’Toole) pp. 17–22. (ICRISAT: Patancheru, India)

Blum A, Ebercon A (1981) Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Science 21, 43–47.
CrossRef |

Bouman BAM, Wang H, Yang X, Zhao J, Wang C (2002) Aerobic rice (Han Dao): a new way of growing rice in water-short areas. In ‘Proceedings of the 12th International Soil Conservation Organization Conference’. pp. 175–181. (Tsinghua University Press: Beijing)

Bouman BAM, Peng S, Castaneda AR, Visperas RM (2005) Yield and water use of irrigated tropical aerobic rice systems. Agricultural Water Management 74, 87–105.
CrossRef |

Charng Y, Liu H, Liu N, Hsu F, Ko S (2006) Arabidopsis Hsa32, novel heat shock protein, is essential for acquired thermo tolerance during long term recovery after acclimation. Plant Physiology 140, 1297–1305.
CrossRef | CAS | PubMed |

Clark R, Maccurdy R, Jung J, Shaff J, McCouch SR, Aneshansley D, Kochian L (2011) 3-Dimensional root phenotyping with a novel imaging and software platform. Plant Physiology 156, 455–465.
CrossRef | CAS | PubMed |

DaCosta M, Huang B (2007) Changes in antioxidant enzyme activities and lipid peroxidation for bentgrass species in response to drought. Journal of the American Society for Horticultural Science 132, 319–326.

Ekanayake IJ, Steponkus PL, DeDatta SK (1989) Spikelet sterility and flowering response of rice to water stress at anthesis. Annals of Botany 63, 257–264.

Ekanayake IJ, Steponkus PL, De Datta SK (1990) Sensitivity of pollination to water deficits at anthesis in upland rice. Crop Science 30, 310–315.
CrossRef |

Fokar M, Nguyen HT, Blum A (1998) Heat tolerance in spring wheat. I. Estimating cellular thermo tolerance and its heritability. Euphytica 104, 1–8.
CrossRef |

Gowda VRP, Henry A, Akira Y, Shashidhar HE, Serraj R (2011) Root biology and genetics improvement for drought avoidance in rice. Field Crops Research 122, 1–13.
CrossRef |

Gowda VRP, Henry A, Vadez V, Shashidhar HE, Serraj R (2012) Water uptake dynamics under progressive drought stress in diverse accessions of the OryzaSNP panel of rice (Oryza sativa). Functional Plant Biology 39, 402–411.
CrossRef |

Guilioni L, Wery J, Tardieu F (1997) Heat stress-induced abortion of buds and flowers in pea: is sensitivity linked to organ age or to relations between reproductive organs? Annals of Botany 80, 159–168.
CrossRef |

Guilioni L, Wery J, Lecoeur J (2003) High temperature and water deficit may reduce seed number in field pea purely by decreasing plant growth rate. Functional Plant Biology 30, 1151–1164.
CrossRef |

Henry A, Gowda VRP, Torres RO, McNally K, Serraj R (2011) Genetic variation in root architecture and drought response in Oryza sativa: rainfed lowland field studies of the OryzaSNP panel. Field Crops Research 120, 205–214.
CrossRef |

Impa SM, Nadaradjan S, Boominathan P, Shashidar GP, Bindumadhava H, Shushshayee MS (2005) Carbon isotope discrimination accurately reflects variability in WUE measured at a whole-plant level in rice. Crop Science 45, 2517–2522.
CrossRef | CAS |

IPCC (2013) ‘Climate change 2013: the physical science basis. Working Group I Contribution to the IPCC 5th Assessment Report.’ (IPCC: Geneva, Switzerland)

Jagadish SVK, Muthurajan R, Rang ZW, Malo R, Heuer S, Bennett J, Craufurd PQ (2011) Spikelet proteomic response to combined water deficit and heat stress in rice (Oryza sativa cv. N22). Rice 4, 1–11.
CrossRef |

Jung JK, McCouch SR (2013) Getting to the roots of it: genetic and hormonal control of root architecture. Frontiers in Plant Science 4, 1–32.
CrossRef |

Keuls M (1952) The use of the Studentized range in connection with an analysis of variance. Euphytica 1, 112–122.
CrossRef |

Kumar G, Krishnaprasad BT, Savitha M, Gopalakrishna R, Mukhopadhyay K, Ramamohan G, Udayakumar M (1999) Enhanced expression of heat shock proteins in thermotolerant lines of sunflower and their progenies selected on the basis of temperature induction response (TIR). Theoretical and Applied Genetics 99, 359–367.
CrossRef |

Larkindale J, Huang BR (2005) Effects of abscisic acid, salicylic acid, ethylene and hydrogen peroxide in thermotolerance and recovery for creeping bent grass. Plant Growth Regulation 47, 17–28.
CrossRef | CAS |

Larkindale J, Hall JD, Knight MR, Vierling E (2005) Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermo-tolerance. Plant Physiology 138, 882–897.
CrossRef | CAS | PubMed |

Leopold AC, Willing RP (1983) Evidence of toxicity effects of salt on membranes. In ‘Plant improvement for irrigated crop production under increasing saline conditions’. (Eds RC Staples, GH Toenniessen) pp. 678–685. (John Wiley & Sons: New York)

Li Z, Mu P, Li C, Zhang H, Li Z, Gao Y, Wang X (2005) QTL mapping of root traits in a doubled haploid population from a cross between upland and lowland japonica rice in three environments. Theoretical and Applied Genetics 110, 1244–1252.
CrossRef | CAS | PubMed |

Liu JX, Liao DQ, Oane R, Estenor L, Yang XE, Li ZC, Bennett J (2006) Genetic variation in the sensitivity of anther dehiscence to drought stress in rice. Field Crops Research 97, 87–100.
CrossRef |

Martineau JR, Specht JE, Williams JH, Sullivan CY (1979) Temperature tolerance in soybeans. I. Evaluation of a technique for assessing cellular membrane thermostability. Crop Science 19, 75–78.
CrossRef |

Matsui T, Omasa K, Horie T (2000) High temperature at flowering inhibits swelling of pollen grains, a driving force for anther dehiscence in rice (Oryza sativa L.). Plant Production Science 3, 430–434.
CrossRef |

Matsui T, Omasa K, Horie T (2001) The difference in sterility due to high temperatures during the flowering period among japonica rice varieties. Plant Production Science 4, 90–93.
CrossRef |

Mittler R (2006) Abiotic stress, the field environment and stress combination. Trends in Plant Science 11, 15–19.
CrossRef | CAS | PubMed |

Murty PSS, Murty KS (1982) Spikelet sterility in relation to nitrogen and carbohydrate contents in rice. Indian Journal of Plant Physiology 25, 40–48.

Newman D (1939) The distribution of range in sample from a normal population expressed in terms of an independent estimate of standard deviation. Biometrika 31, 20–30.

Nguyen HT, Babu RC, Blum A (1997) Breeding for drought resistance in rice: physiology and molecular genetics consideration. Crop Science 37, 1426–1434.
CrossRef |

O’Toole JC, Namuco OS (1983) Role of panicle exertion in water-stress induced sterility. Crop Science 23, 1093–1097.
CrossRef |

Pantuwan G, Fukai S, Cooper M, Rajatasereekul S, O’Toole JC (2002a) Yield response of rice (Oryza sativa L.) genotypes to drought under rainfed lowlands. 1. Grain yield and yield components. Field Crops Research 73, 153–168.
CrossRef |

Pantuwan G, Fukai S, Cooper M, Rajatasereekul S, O’Toole JC (2002b) Yield response of rice (Oryza sativa L.) genotypes to drought under rainfed lowlands 2. Selection of drought resistant genotypes. Field Crops Research 73, 169–180.
CrossRef |

Passioura JB (1986) Resistance to drought and salinity: avenues for improvement. Australian Journal of Plant Physiology 13, 191–201.
CrossRef |

Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, Centeno GS, Khush GS, Cassman KG (2004) Rice yields decline with higher night temperature from global warming. Proceedings of the National Academy of Sciences of the United States of America 101, 9971–9975.
CrossRef | CAS | PubMed |

Peng S, Bouman BAM, Visperas RM, Castaneda A, Nie L, Park HK (2006) Comparison between aerobic and flooded rice in the tropics: agronomic performance in an eight-season experiment. Field Crops Research 96, 252–259.
CrossRef |

Prasad PVV, Boote KJ, Allen JLH, Sheehy JE, Thomsa JMG (2006) Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress. Field Crops Research 95, 398–411.
CrossRef |

Price AH, Tomos AD (1997) Genetic dissection of root growth in rice (Oryza sativa L.): II: mapping quantitative trait loci using molecular markers. Theoretical and Applied Genetics 95, 143–152.
CrossRef | CAS |

Rang ZW, Jagadish SVK, Zhou QM, Craufurd PQ, Heuer H (2011) Effect of high temperature and water stress on pollen germination and spikelet fertility in rice. Environmental and Experimental Botany 70, 58–65.
CrossRef |

Reynolds M, Tuberosa R (2008) Translational research impacting on crop productivity in drought-prone environments. Current Opinion in Plant Biology 11, 171–179.
CrossRef | PubMed |

Reynolds MP, Balota M, Delgado MIB, Amani I, Fischer RA (1994) Physiological and morphological traits associated with spring wheat yield under hot, irrigated conditions. Australian Journal of Plant Physiology 21, 717–730.
CrossRef |

Saghai-Maroof M, Solima KM, Jorgenson RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proceedings of the National Academy of Sciences of the United States of America 81, 8014–8018.
CrossRef | CAS | PubMed |

Saini HS, Westgate ME (1999) Reproductive development in grain crops during drought. Advances in Agronomy 68, 59–96.
CrossRef |

Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002a) A proteomic approach to analyzing drought- and salt-responsiveness in rice. Field Crops Research 76, 199–219.
CrossRef |

Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002b) Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2, 1131–1145.
CrossRef | CAS | PubMed |

Sandhu N, Jain S, Kumar A, Mehla BS, Jain R (2013) Genetic variation, linkage mapping of QTL and correlation studies for yield, root, and agronomic traits for aerobic adaptation. BMC Genetics 14, 104
CrossRef | PubMed |

Senthil-Kumar M, Udayakumar M (2004) Development of thermotolerant tomato (Lycopersicon esculentum Mill.) lines: an approach based on mutagenesis. Journal Plant Biology (India) 31, 139–148.

Senthil-Kumar M, Srikanthbabu V, Mohanraju B, Kumar G, Shivaprakash N, Udayakumar M (2003) Screening of inbred lines to develop a thermotolerant sunflower hybrid using the temperature induction response (TIR) technique: a novel approach by exploiting residual variability. Journal of Experimental Botany 54, 2569–2578.
CrossRef | CAS | PubMed |

Senthil-Kumar M, Kumar G, Srikanthbabu V, Udayakumar M (2007) Assessment of variability in acquired thermo tolerance: potential option to study genotypic response and the relevance of stress genes. Journal of Plant Physiology 164, 111–125.
CrossRef | CAS | PubMed |

Shannon CE, Weaver W (1949) ‘The mathematical theory of communication.’ (University of Illinois Press: Urbana, IL, USA)

Sheshshayee MS, Abou-Kheir E, Sreevathsa R, Srivastava N, Mohanraju B, Nataraja KN, Prasad TG, Udayakumar M (2011a) Phenotyping for root traits and their improvement through biotechnological approaches to sustaining crop productivity. In ‘Root genomics’. (Eds A C de Oliveira, RK Varshney) pp. 205–232. (Springer-Verlag: Berlin)

Sheshshayee MS, Shashidhar GP, Madhura JN, Beena R, Prasad TG, Udayakumar M (2011b). Phenotyping groundnuts for adaptation to drought. In ‘Phenotyping document II.2 Legumes’. pp. 371–391. (Eds P. Mannevaux, J.-M. Ribau). (CIMMYT: Apdo, Mexico)

Sheshshayee MS, Mohankumar MV, Raju BR, Pratibha MD, Rajanna MP, Mohanraju B, Udayakumar M (2013) Enhancing water use efficiency besides effective use of water is a potential strategy in developing rice cultivars suitable for semi-irrigated aerobic cultivation. In ‘International dialogue on perception and prospects of designer rice’. (Eds K Muralidharan, EA Siddiq) pp. 261–272. (Society for Advancement of Rice Research, Directorate of Rice Research: Hyderabad, India)

Sikder HP, Gupta DKD (1976) Physiology of grain in rice. Indian Agriculture 20, 133–141.

Songsri P, Joglo S, Vorasoot N, Akkasaeng C, Patanothai A. Holbrook CC (2008) Root distribution of drought resistant peanut genotypes in response to drought. Journal Agronomy & Crop Science 194, 92–103.
CrossRef |

Songsri P, Jogloy S, Holbrook CC, Kesmala T, Vorasoot N, Akkasaeng C, Patanothai A (2009) Association of root, specific leaf area and SPAD chlorophyll meter reading to water use efficiency of peanut under different available soil water. Agricultural Water Management 96, 790–798.
CrossRef |

Srikanthbabu V, Kumar G, Krishnaprasad BT, Gopalakrishna R, Savitha M, Udayakumar M (2002) Identification of pea genotypes with enhanced thermotolerance using temperature induction response (TIR) technique. Journal of Plant Physiology 159, 535–545.
CrossRef | CAS |

Sullivan CY (1972) Mechanisms of heat and drought resistance in grain sorghum and method of measurements. In ‘Sorghum in the seventies’. (Eds NGP Rao, LR House) pp. 247–264. (Oxford and IBH, New Delhi, India)

Tripathy JN, Zhang J, Robin S, Nguyen TT (2000) QTLs for cell- membrane stability mapped in rice (Oryza sativa L.) under drought stress. Theoretical and Applied Genetics 100, 1197–1202.
CrossRef | CAS |

Tuberosa R (2012) Phenotyping for drought tolerance of crops in the genomics era. Frontiers in Physiology 3, 1–26.

Udayakumar M, Devendra R, Ramaswamy GS, Nageswara Rao RC, Roy SA, Gangadhar GC, Aftab Hussain IS, Wright GC (1998) Measurement of transpiration efficiency in field condition. Indian Journal of Plant Physiology and Biochemistry 1, 69–75.

Uga Y, Sugimoto K, Ogawa S, Rane J, Ishitani M, Hara N, Kitomi Y, Inukai Y, Ono K, Kanno N, Inoue H, Takehisa H, Motoyama R, Nagamura Y, Wu J, Matsumoto T, Takai T, Okuno K, Yano M (2013) Control of root system architecture by DEEPER ROOTING 1 increases rice yield under drought conditions. Nature Genetics 45, 1097–1102.
CrossRef | CAS | PubMed |

Wang K, Jiang Y (2007) Antioxidant responses of creeping bentgrass roots to water logging. Crop Science 47, 232–238.
CrossRef | CAS |

Wang W, Vinocur B, Shoseyov O, Altman A (2004) Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in Plant Science 9, 244–252.
CrossRef | CAS | PubMed |

Wright GC, Nageswara Rao RC (1994) Peanut water relations. In ‘The groundnut crop. (Ed. J Smartt) pp. 281–325. (Chapman & Hall: London)

Young TE, Ling J, Geisler-lee CJ, Tanguay RL, Caldwell C, Gallie DR (2001) Developmental and thermal regulation of maize heat shock protein, HSP101. Plant Physiology 127, 777–791.
CrossRef | CAS | PubMed |

Zhu Q, Cao X, Luo Y (1988) Growth analysis in the process of grain filling in rice. Acta Agronomica Sinica [in Chinese with English abstract] 14, 182–192.

Zhu X, Gong H, Chen G, Wang S, Zhang C (2005) Different solute levels in two spring wheat cultivars induced by progressive field water stress at different developmental stages. Journal of Arid Environments 62, 1–14.
CrossRef |

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