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Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
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Crop & Pasture Science

Crop & Pasture Science

Volume 75 Number 1 2024

CP23143Effects of infection with the viral complex alfalfa dwarf disease on productivity traits and coumestrol production in alfalfa plants

Mercedes P. Silva, Daniel H. Basigalup, Stella Maris Chiacchiera, Verónica Trucco, Fabián Giolitti, Eva Mamani, Carolina del Pilar Díaz and Milena E. Manzur 0000-0002-0224-0441

Viral infections generate yield losses in alfalfa due to the impact on growth but also stimulate production of coumestrol, which can exhibit oestrogenic activity in both livestock and human consumers. We investigated the responses of two alfalfa cultivars, focusing on changes in coumestrol content and their relationship with morphological and productivity plant traits. Coumestrol content was highly variable and increased in 3 of 13 genotypes in response to viral infections. High coumestrol content was correlated with low viral infection symptoms.

Iron deficiency is prevalent worldwide, causing anaemia in millions of people, but biofortification offers a potential solution. This greenhouse study evaluated the biofortification potential of broccoli, a highly nutritious crop, revealing that foliar application of iron, especially in combination with nitrogen, significantly increased floret weight and diameter while enhancing iron content, antioxidant activity and polyphenol levels. These findings pave the way for biofortification programs.

CP22335Utilisation of unmanned aerial vehicle imagery to assess growth parameters in mungbean (Vigna radiata (L.) Wilczek)

Yiyi Xiong 0000-0001-9238-6200, Lucas Mauro Rogerio Chiau 0009-0006-1398-8486, Kylie Wenham 0000-0003-2784-4513, Marisa Collins 0000-0001-6450-3078 and Scott C. Chapman 0000-0003-4732-8452

Application of drones with affordable red–green–blue cameras has been explored in multiple crops for different purposes. However, this study is the first to estimate mungbean biomass using drone imagery in multiple field seasons. Our results showed that drone-based red–green–blue cameras can replace destructive and time-consuming ground growth measurements in the paddock and provide high accuracy in predicting mungbean biomass at pre-flowering stages. This research helps to understand mungbean physiology and explore management practices in different seasonal conditions in the future.

CP23047Phenotypic variations in M2 generation by ethyl methanesulfonate mutagenesis in lucerne (Medicago sativa L.)

S. S. Jade, V. N. Gaikwad, S. P. Jadhav, P. S. Takawale and R. A. Bahulikar 0000-0002-0442-4607

Lucerne (Medicago sativa L.) is a highly nutritious fodder legume and its breeding requires a genetically divergent gene pool. As lucerne has been introduced in India, limited germplasm is available. A high biomass-producing line has been mutagenised with a chemical mutagen, ethyl methanesulfonate (EMS), to introduce variability. The resulting mutant population showed wide variations for most morphological characters which can be further utilised in breeding programs to improve the lucerne for various traits.

CP23109The growth habits of tropical legumes affect the nutritive herbage value more than harvesting frequency

Tafnes B. Sales-Silva 0000-0002-2883-8433, Mércia V. F. dos Santos, Osniel F. de Oliveira, Pedro H. F. da Silva 0000-0002-0794-4499, Evaristo J. O. de Souza, Djalma E. Simões Neto, Abdias J. da Silva Neto and Márcio V. da Cunha

This paper discusses the nutritive herbage value of tropical forage legumes with different growth habits subject to 60 and 90-day harvest regimes. Compared to the butterfly pea herb and the sub-shrub stylo cv. Campo Grande, the shrub hedge lucerne was characterised by higher contents of insoluble proteins and carbohydrates and enormous concentrations of secondary metabolites, even greater when harvested at 90 days. Prolonged harvest intervals must be avoided in shrub hedge lucerne management for animal feeding.

CP23177Identification of environment similarities using a crop model to assist the cultivation and breeding of a new crop in a new region

Yashvir S. Chauhan 0000-0002-0135-6362, Doug Sands, Steve Krosch, Peter Agius, Troy Frederiks, Karine Chenu 0000-0001-7273-2057 and Rex Williams

Crop yield variability due to weather patterns in subtropical and tropical rainfed environments is a significant concern. To address this issue, a framework using the APSIM model has been proposed to improve crop resilience. The framework was tested on pigeonpea (Cajanus cajan L. Millsp.) in Australia’s northern grains region and found that yield variability is linked to heat and drought frequencies. It provides valuable insights into environmental similarity, which can then be used to aid in crop improvement strategies.

White mould (Sclerotinia sclerotiorum) causes major yield losses on common bean (Phaseolus vulgaris) and no commercial cultivar with high physiological resistance along with high yield and market-adapted grain is currently available. Studies with 34 common bean genotypes with a wide range of agronomic traits and grain types highlighted new host stem, leaf, and cotyledon resistances highly applicable for use in common bean breeding programs and/or even direct deployment into field situations where white mould is problematic on common bean.

CP23149Plant species, nitrogen status and endophytes are drivers of soil microbial communities in grasslands

Susanne Rasmussen, Anthony J. Parsons, Julia Russell, Daniel A. Bastías 0000-0002-0522-5538 and Qianhe Liu 0000-0002-2791-2187

Plants, environmental nutrients and endophytes can affect soil microbiota. Effects of these factors on grassland soil microbial communities were studied and results showed that plant species, nitrogen status and Epichloë endophytes alter the abundance of phytopathogens, beneficial plant microbes and microorganisms associated with the nitrogen cycle.

CP23250Humic and fulvic acid influence the morphophysiological and biochemical properties of cowpea (Vigna unguiculata) under water deficit

Angela Lucena Nascimento de Jesus 0000-0003-0101-0924, Lucas Vinícius Pierre de Andrada, Luiz Filipe dos Santos Silva, Natália Alves Santos, Fabiana Barbosa Cruz, Nerissa Carolina Amosse Cumbana, Rafael Anchieta de Oliveira 0009-0002-9456-0377 and Evaristo Jorge Oliveira de Souza 0000-0002-2206-414X

The use of products natural origin has gained great prominence to produce agricultural species. Considering the importance of cowpea (Vigna unguiculata), and that it is a crop sensitive to water deficit, we show that the combined use of humic and fulvic acid provides good agronomic performance in common bean plants under severe water stress. The results obtained showed a good performance of the legume, these results can help in decision making in agricultural production in dry regions.

In this pot study undertaken at two locations, with two populations each of B. diandrus and L. rigidum, water stress was applied applied from GS31 or GS60 stage until maturity. There was a well-watered treatment (Control). The stress vs well-watered treatments were assessed for seed dormancy and the gene expression of ABA1 and GA20ox using quantitative polymerase chain reaction. The seeds of GS31 were most dormant and water stress also altered the expression of GA20ox, which is associated with level of seed dormancy in these weed species.

Wheat is vital to meeting human dietary needs and enhancing worldwide food security. This research focused on two crucial traits, plant height and spike length, which directly impact wheat yield potential. By identifying specific genomic regions and candidate genes associated with these traits, this study paves the way for future targeted breeding efforts and the development of molecular marker-assisted techniques, offering hope for improved wheat varieties to enhance global food security.

Unlocking the full potential of soybean for improved nutrition is a critical issue in agriculture. Addressing the challenge of inherent antinutritional factors and to aiming to boost nutrient content in soybean, our research focuses on leveraging both traditional plant breeding and cutting-edge biotechnological interventions. Our findings showcase a significant breakthrough in enhancing the nutritional profile of soybean, offering a promising path towards addressing global food security and nutrition challenges.

CP22287Moister soils at elevated CO2 stimulate root biomass but suppress aboveground biomass production in Lolium perenne

Mark J. Hovenden 0000-0001-7208-9700, Amanda L. Sinclair, Rose E. Brinkhoff 0000-0001-9089-6661, Kate Stevenson, Zachary A. Brown, Meagan Porter, Anna Flittner, Marion Nyberg and Richard P. Rawnsley 0000-0001-5381-0208

Elevated carbon dioxide (CO2) concentrations can stimulate plant growth, partly by reducing plant water use, so there are reasons to believe that the stimulating effects of CO2 might vary strongly with seasonal water supply. We manipulated CO2 concentration and water supply in a ryegrass pasture and found that water limitation in any season interfered with the growth stimulation caused by elevated CO2, all of which occurred in the roots. Therefore, rising carbon dioxide concentrations might not stimulate aboveground pasture production.

CP23091Remote detection of Fusarium crown rot in broadacre bread wheat and durum wheat through use of aerial imagery

M. Buster 0000-0001-9760-8765, S. Simpfendorfer, C. Guppy 0000-0001-7274-607X, M. Sissons 0000-0003-3396-2911, M. K. Tighe and R. J. Flavel 0000-0001-7867-2104

This paper addresses the wheat disease Fusarium crown rot (FCR), caused by the fungal pathogen Fusarium pseudograminearum. The study investigates multiple methods (multispectral and thermal) for remote detection of FCR. The paper outlines strengths and limitations of particular remote technologies in their ability to distinguish between infected and uninfected crops.

Landraces are a potential source of genetic diversity and provide useful genetic resources to cope with current and future challenges in crop breeding. A worldwide diversity panel of 200 durum wheat accessions were evaluated under drought and irrigated conditions. Ideal accessions with good levels of agronomic traits, and high mean yield and stability were identified. The findings are useful for selective breeding for specific traits as well as for enhancing the genetic basis of durum wheat breeding programs.

CP23247A uniform gene and chromosome nomenclature system for oat (Avena spp.)

Eric N. Jellen 0000-0002-7906-4845, Charlene P. Wight 0000-0003-1410-5631, Manuel Spannagl, Victoria C. Blake, James Chong, Matthias H. Herrmann, Catherine J. Howarth, Yung-Fen Huang, Jia Juqing, Andreas Katsiotis, Tim Langdon, Chengdao Li 0000-0002-9653-2700, Robert Park, Nicholas A. Tinker and Taner Z. Sen 0000-0002-5553-6190

This article provides a standard framework for naming oat chromosomes, genomes, genes, gene models and markers. It represents the work of the International Oat Nomenclature Committee (IONC) and anticipates the impending publication of multiple whole-genome oat sequence assemblies.

CP22344Water use dynamics of dryland wheat grown under elevated CO2 with supplemental nitrogen

Shihab Uddin 0000-0002-1257-4269, Shahnaj Parvin, Roger Armstrong 0000-0002-4728-9935, Glenn J. Fitzgerald, Markus Löw, Alireza Houshmandfar, Ehsan Tavakkoli, Sabine Tausz-Posch, Garry J. O’Leary and Michael Tausz

We investigated elevated [CO2] and supplemental N interactions on growth, yield and water use dynamics of two dryland wheat cultivars in a free air CO2 enrichment facility. Increased leaf-level water use efficiency under elevated [CO2] was initially offset by increasing biomass; however, by end of season, cumulative water use similar to that under ambient [CO2] indicated the dominating effect of prevailing seasonal conditions. Cultivar yield responses to supplemental N were associated with ability to maintain greater post-anthesis leaf N, chlorophyll content and canopy cover, which together with higher net assimilation rates improved assimilate supply to developing grains.

CP23034Environmental consequences of a consumer shift from dairy- to soy-based products

Aaron T. Simmons 0000-0002-3638-4945, Miguel Brandão, Zita Ritchie and Guy Roth

Dairy production is considered unsustainable due to the carbon and water footprints of current production. We assessed the consequences of replacing dairy products and co-products with plant-based alternatives and found that water savings would be negligible and greenhouse gas reductions are not reflected in the carbon footprint of current production. These results illustrate that policies developed to improve the sustainability of agricultural production need to be based on the consequences of a change, not the impacts of current production.

CP23147Genome-wide identification and expression profiling of 4-coumarate:coenzyme A ligase genes influencing soybean isoflavones at the seedling stage

Zhenhong Yang, Xu Wu, Jinglin Ma, Ming Yuan, Yuhang Zhan, Yonguang Li, Haiyan Li, Weili Teng 0000-0001-8776-6049, Xue Zhao 0000-0003-3362-1471 and Yingpeng Han 0000-0002-9829-6588

Soybean isoflavone is an important quality component of soybean. We examined the genes of enzymes having influence on the synthesis and accumulation of soybean isoflavone. The results of this study confirm the effectiveness of enzymes related to the soybean isoflavone synthesis pathway and provide a basis for further exploration of new synthetic pathways.

CP23171Transcriptome analysis showed the metabolic pathway of differentially expressed genes (DEGs) in resistant and susceptible soybean (Glycine max) to sclerotinia stem rot (SSR) and candidate gene mining

Dongming Sun, Ruiqiong Li, Jinglin Ma, Shuo Qu, Ming Yuan, Zhenhong Yang, Changjun Zhou, Junrong Xu, Yuhang Zhan, Xue Zhao 0000-0003-3362-1471, Yingpeng Han 0000-0002-9829-6588 and Weili Teng 0000-0001-8776-6049

Sclerotinia stem rot (SSR) is one of the diseases that seriously affect soybean yield, leading to heavy losses all over the world. In this study, transcriptome sequencing analysis of resistant variety ‘Maple Arrow’ and susceptible variety ‘Hefeng25’ was conducted to understand the resistance mechanism of resistant and susceptible soybean varieties to SSR and to look for candidate genes. In total, 31 haplotypes of 12 genes were screened. The result provides new ideas for future research on SSR resistance of soybean.

CP23110Should crop sequences in Western Australia include more lupins?

C. d’Abbadie, S. Kharel, R. Kingwell 0000-0003-0324-9488 and A. Abadi Ghadim

Replacing canola with lupins as the break crop in rotations at a range of locations in Western Australia’s grainbelt is found to be environmentally attractive, due to lower emissions of greenhouse gases in crop sequences that include lupins. However, the higher gross margins from canola production cause lupins to not be a preferred break crop option in a majority of locations. Even in the face of various reasonable and plausible incentives to favour lupin production, lupins still remain economically unattractive at most locations.

Australian grain producers may need to account for greenhouse gas emissions associated with their use of nitrogenous fertilisers. Accordingly, nitrogen fertiliser application strategies should encompass economic and environmental impacts. This study uses simulation modelling to examine gross margins and emissions associated with four different nitrogen strategies at 14 locations in the Western Australian grainbelt for different cropping intensities. Profitable strategies that generate only moderate emissions often focus on maximising the gross margin of crop production rather than crop yield.

Changes in light intensity and nitrogen availability influenced the growth of C4 forage species in a silvopastoral system, which is a diverse and sustainable land use alternative that aggregates value to end-products. The five species compared in the current study adopted different mechanisms involving shoot traits, when resources, light and nitrogen all varied, with different impacts on yield. Agroforestry practitioners may want to choose forages that are more likely to maintain biomass yield as trees grow.

CP23201Is animal saliva a prominent factor in pasture regrowth?

Danica Parnell 0000-0002-0861-6497, Andrew Merchant and Lachlan Ingram

Research has occurred over 100 years on the effects that grazing livestock have on pasture systems globally, however, the influences that it has on regrowth rates above and belowground remains relatively unclear due to contradictory results often evident. Prior studies have highlighted that the answers may lie in the effect of livestock saliva, and the authors of this review discuss the current knowledge such as the impact saliva has on plants, and the greater applications of this knowledge for producers.

CP23040Genotype-dependent responses of Andean and Coastal quinoa to plant population density for yield and its physiological determinants in Northwest Argentina

Juan José Agüero 0000-0003-1164-3055, Martín Moisés Acreche 0000-0002-3963-8883, Silvia Susana Sühring, Héctor Daniel Bertero and Ramiro Néstor Curti 0000-0001-8353-8858

Can we reduce yield gaps in quinoa by adjusting the plant population density for the most adapted genotype for a given environment? An optimal plant population density is hard to find in quinoa due to its phenotypic plasticity. In Northwest Argentina, Andean genotypes achieved the highest yields in the Highlands at conventional plant population density, whereas Coastal genotypes showed higher yields than Andean genotypes in the Dry Valleys, even when maintained at half the conventional plant population density in both locations.

CP23157Seed row placement relative to the previous crop stubble row can harness systems benefits on water repellent sands

Therese McBeath 0000-0001-6423-367X, Vadakattu Gupta, Jack Desbiolles, Bill Davoren and Rick Llewellyn

Successful crop establishment is a key constraint to productive agricultural landscapes in the semi-arid cropping zone of southern Australia. Seeder-based innovations that modify the placement of the crop row relative to the previous row can improve crop establishment, weed competition and yield in a limited set of scenarios. Further innovation is required to generate predictable landscape level benefits accruing from improved crop establishment, in soils that often suffer low and uneven crop establishment numbers.

CP23214Field screening for heat-stress tolerance of floret fertility in wheat (Triticum aestivum and T. durum)

Livinus Emebiri 0000-0002-5261-4552, Million F. Erena, Kerry Taylor, Shane Hildebrand, Marco Maccaferri and Nicholas C. Collins

Heat waves are projected to become more frequent during reproductive development of wheat, reducing grain yield. In experiments involving sowing late and using auricle distance to identify tillers exposed to heat events, we found substantial genetic variation for tolerance of grain set to natural heat waves. A validation study with durum wheat lines showed good agreement in ranking at the extremes of tolerance. The protocol could provide a low-cost method for evaluating wheat heat tolerance under commercial field production conditions.

CP23061Performance of young endophyte-free and endophyte-infected tall fescue (Festuca arundinacea) plants under partial and total submergence

Lucas R. Petigrosso 0000-0002-5865-3181, Osvaldo R. Vignolio 0000-0003-4862-9273, Pedro E. Gundel 0000-0003-3246-0282, María G. Monterubbianesi 0000-0002-3048-8313 and Silvia G. Assuero 0000-0001-7817-819X

Tall fescue is a valuable temperate forage grass, commonly associated with a beneficial fungus, and can be found in flood-prone areas. We found no evidence that the presence of the fungus improves the performance of young plants under flooding. However, given that the frequency of infected plants tends to increase with time, further research should explore whether beneficial effects are found with longer flooding, different plant developmental status, or co-occurrence of flooding with other stress factors.

CP23032Use of interpretive machine learning and a crop model to investigate the impact of environment and management on soybean yield gap

Alireza Nehbandani 0000-0001-5324-260X, Patrick Filippi, Parisa Alizadeh-Dehkordi, Amir Dadrasi 0000-0002-4809-657X and Afshin Soltani

We used a combination of machine learning approaches and the SSM-iCrop2 simulation model to identify critical climate and management factors affecting soybean yield. The XGBoost models adequately described the observed changes in soybean yield, with 15 climatic and management variables identified as affecting soybean yield. The results indicate that water limitation is the most important factor causing yield gap in the study area.

CP22360Phenotypic variation of Kikuyu grass (Cenchrus clandestinus) across livestock production farms in Colombian highlands is explained by management and environment rather than genetic diversity

Javier Castillo Sierra 0000-0003-0797-3908, Ivania Cerón-Souza, Yesid Avellaneda Avellaneda, Edgar Augusto Mancipe Muñoz and Juan de Jesús Vargas Martínez

Kikuyu grass is an introduced species in Colombia that supports dairy and beef production feeding systems at high altitudes. It has shown broad phenotypic variation, and to enable proper management, we sought to determine the importance of genetic diversity and/or environmental variables in explaining this variation. The effect of genetic structure was negligible; rather, location, altitude and 13 covariables of pasture management and soil traits explained 40% of the variation.

CP23183Role of RNA interference in drought stress management: physiological, biochemical and molecular approach

Naveen Sihag 0000-0001-9113-7333, Tushadri Singh 0009-0001-0582-7096, Sonia Sheoran, Omvir Singh, Rekha Malik, Lokendra Kumar and Jogendra Singh

Plants respond to various abiotic stress (drought) tolerance through physiological, biochemical and molecular mechanisms. RNA interference (RNAi) plays a crucial role in regulating molecular response to stress. This review discusses, in addition to RNAi, the fine tuning of miRNAs using CRISPR/Cas9 to enhance the precision of gene expression regulation and increase plant tolerance to drought stress.

This article belongs to the collection Abiotic Stress Tolerance vs Climate Change.

CP23172Yield and nutritional quality of intercropped forages for organic production in a hot-summer Mediterranean oak grassland ecosystem

Javier Plaza 0000-0002-8908-1606, Marco Criado 0000-0001-5509-7573, M. Remedios Morales-Corts 0000-0001-6736-5854, Rodrigo Pérez-Sánchez 0000-0001-5170-4698, M. Ángeles Gómez-Sánchez 0000-0002-9127-495X, Beatriz Vázquez-de-Aldana 0000-0001-6549-3545, Iñigo Zabalgogeazcoa 0000-0002-9524-7799 and Carlos Palacios 0000-0001-5876-6226

Forage cultivation is essential for feeding ruminants, especially in resource-limited ecosystems, and these forages should be grown within environmentally sustainable systems. Organic forage mixtures meet this requirement and offer the possibility of simultaneously reaching higher forage yields and better nutritional quality. By assessing the viability of six organic forage mixtures in an oak grassland environment, we provide farmers with information to choose the best option to fit their specific agroclimatic conditions.

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